초록

본 연구에서 1단계 연구개발결과 1차년도에 노화기인 근 감소 transcriptome DB를 구축 완료하였음. 그리고 2차년도에 수행한 각종 분석결과 노화 기인 sarcopenia 기전 분석 및 마커 발굴과 기술적 검증이 완료되었음. 3차년도에는 식이제한에 의한 노화제어 transcriptome DB를 구축완료하였고 이에 대한 비교분석 및 마커발굴과 기술적 검증이 완료되었음. 최종적으로 노화기인, 노화제어 splicing variant 마커 발굴을 완료함.

Abstract ▼

Discovery of markers for loss of muscle mass and muscular degeneration by aging is essential to develop methods for prevention and cure of aging-associated muscle problems. The purpose of this research proposal is to find these markers and to develop a screen

Discovery of markers for loss of muscle mass and muscular degeneration by aging is essential to develop methods for prevention and cure of aging-associated muscle problems. The purpose of this research proposal is to find these markers and to develop a screening system for natural products to slow down aging-associated muscular dysfunctions.
In this study, we will establish transcriptome DB and information of chromatin modification related to the aging process in mouse gastrocnemius muscle during natural aging process. In addition, to screen potential natural products showing a preventive effects on aging-associated sacopenia including muscle loss, muscle degeneration, and muscular dysfunction, we will build a cell-based screening system.
1. Measurements of transcriptome DB from mouse skeletal muscle tissues during natural aging process, and from caloric restriction (CR)-treated muscle tissues:
The transcriptome DB will be analyzed for finding major signaling pathways related to muscle degeneration, loss of myocytes, and decline of functions to discover markes for age-associated sacopenia.
2. Measurements of global modification of major histone proteins in chromatin:
Major chromatin factors determining aging-associated sacopenia will be found with association studies with global gene expression data using a linear regression model and a discovery model. Establishment gene expression regulation network of muscle degeneration.
3. Developments of a cell-based screening system for natural products showing preventive effects against aging-associated sacopenia:
We will use the markers developed from 1 and 2 to efficiently screen natural products showing preventive effects on aging-associated muscular dysfunction.
Research subjects:
(1) Measurement and establishment of transcriptome DB and chromatin modification in mouse skeletal muscle under natural aging process
(2) Isolation and analysis of markers for sacopenia
(3) Measurement of global histone modification altered by CR
(4) Development of a cell-based screening system for natural products showing anti-muscular dysfunction associated with the aging process including myocyte loss and muscular degeneration
Based on the results of our studies, we will suggest markers for prevention of age-related sacopenia including muscle degeneration and loss which could be applicable in the human body. In addition, we will build a cell-based screening system to accelerate new discoveries for natural products showing preventive effects againg aging-associated sacopenia.