초록

Ⅳ. 연구개발결과
● 나노 전달체를 이용한 다양한 리프로그래밍 방법을 개발
● 그 과정에서 중간단계의 역분화세포를 수립
● 수립된 다양한 단계의 만능줄기세포의 유전자 프로파일을 비교하여, 완전한 리프로그래밍을 위해서는 줄기세포의 유지에 관련된 유전자의 메카니즘을 밝힘.
● episomal 벡터를 이용한 안전한 리프로그래밍 기술 개발하여 외래유전자 삽입 없는 인간 유도만능줄기세포를 수립하였음.

Abstract ▼

In this study, we have generated many different kinds of induced pluripotent stem (iPS) cells using a new reprogramming method (protein and plasmid vector system using nano-carrier), which provide exogene-free pluripotent stem cells. Finally, we have generated several exogene-free human iPS cells, w

In this study, we have generated many different kinds of induced pluripotent stem (iPS) cells using a new reprogramming method (protein and plasmid vector system using nano-carrier), which provide exogene-free pluripotent stem cells. Finally, we have generated several exogene-free human iPS cells, which were also could be induced to differentiation into neural stem cells. These exogene-free human iPS cells will further differentiated into many different lineage. This system will provide the possibility of the technology to be used in the therapeutic application for treatment of patient with incurable disease. Our results also provide the information of molecular and biochemical mechanism of reprogramming of somatic cells and differentiation of iPS cells and also provide the possibility of the technology to be used in the therapeutic application for treatment of patient with incurable disease.