초록 ▼

1차년도: 마이크로중력 (μG) 환경 모사지상실험 시스템확립
1) μG환경 PCG 벤치마킹 및 국제협력추진
- μG활용 단백질결정성장(PCG) 선행연구 벤치마킹
- 국제우주정거장 (ISS) 실험모듈 공동활용을 위한 국제 협력 추진
2) 모사 μG 실험을 위한 세포배양 시스템확립
- 회전세포배양 (Rotatory Cell Culture System, RCCS) 시스템 구축
- 3D-Clinostat 제작 및 운영 시스템 구축
2차년도: 국제우주정거장(ISS) μG 환경 미션 수행 및 지상 모사 μ

1차년도: 마이크로중력 (μG) 환경 모사지상실험 시스템확립
1) μG환경 PCG 벤치마킹 및 국제협력추진
- μG활용 단백질결정성장(PCG) 선행연구 벤치마킹
- 국제우주정거장 (ISS) 실험모듈 공동활용을 위한 국제 협력 추진
2) 모사 μG 실험을 위한 세포배양 시스템확립
- 회전세포배양 (Rotatory Cell Culture System, RCCS) 시스템 구축
- 3D-Clinostat 제작 및 운영 시스템 구축
2차년도: 국제우주정거장(ISS) μG 환경 미션 수행 및 지상 모사 μG 실험
1) μG 모사/비교환경실험 및 PTP단백질 구조연구
- 국제협력을 통한 JAXA-PCG#6 미션 수행
- 방사광을 이용한 고해상도 단백질 구조분석연구
2) 모사 μG 실험 장치 비교분석 및 성능 검증
- 3-D Clinostat 개량 및 모사 μG 성능검증
- RCCS 시스템과 3-D Clinostat 비교 검증
3차년도: 우주바이오 융합연구 및 미래기술연구기획
1) 표적단백질 구조 후속연구
2) RCCS 시스템 개량 및 국산화
3) 첨단큐브위성기술기반 융합연구기획

Abstract ▼

Ⅳ. Results
1) Space-PCG mission utilizing μG environments of JAXA Kibo module at ISS
- A collaborative PCG mission was established between Prof. Kunio MIKI of Kyoto University (JAPAN) and Dr. Dae Gwin Jeong of KRIBB. Prof. MIKI was awarded to access JAXA Kibo module at ISS through JAXA-PCG#6

Ⅳ. Results
1) Space-PCG mission utilizing μG environments of JAXA Kibo module at ISS
- A collaborative PCG mission was established between Prof. Kunio MIKI of Kyoto University (JAPAN) and Dr. Dae Gwin Jeong of KRIBB. Prof. MIKI was awarded to access JAXA Kibo module at ISS through JAXA-PCG#6 mission. Two protein solution samples (PTP-MT1 & wCypA) were sent to JAXA space-PCG lab. Mission started with protein sample evaluation through the year of 2012 and the sample loading in Dec. 2012 (Heisei 24). Sample delivery to ISS was done with Russian ‘Progress’ rocket launch on March 30th 2013. PCG experiments had been done during the 1.5 month stay till 14th May 2013. Space-grown protein crystal samples were delivered to Dr. Jeong at JAXA space-PCG lab on 21st May 2013.
- X-ray synchrotron beamline 5C at Pohang had been used for the diffraction experiments for all space-grown crystals, of which diffraction quality was compromised compared to the best-grown ground crystals, of which wCypA had shown as high resolution as 1.25 Å. We could not obtain any higher resolution data from those space-grown crystals than the best-grown ground crystals. These results were explained and shared with other participant of the same mission JAXA-PCG#6 through the ‘invited’ lecture by Dr. Tae-Sung Yoon at 10th Asian Microgravity Symposium (AMS2014). Hence, the structural analysis of those proteins were done with the best-grown ground crystals (not the space-grown crystals). Anyway, further research involving structure-based drug screen was done to go through the whole value chain of space-PCG, of which results were published in two separate issues of Acta Crystallographica Section D (Biological Science) together with one patent registration related with virtual drug screen.
2) Building Rotatory Cell Culture System (RCCS) for 3-D Cell Culture System
- Originally, RCCS was invented by NASA as cell culture system in simulated μG environments. Comparative studies were done between 3D-clinostat (fabricated by Yonsei University) and RCCS (manufactured by Synthecon, Inc.). 3D-clinostat and RCCS were comparable in their performance as simulated μG devices as confirmed by checking HSP70 protein over-expression in the μG condition. Furthermore, RCCS has demonstrated more application in 3-D cell culture system. In this regard, locally adapted and modified parts were fabricated for 3-D clinostat as well as locally fabricated RCCS. RCCS have been tested not only as a simulated μG device but also as a 3-D cell culture system.
3) Collaborative Framework After 2020, the stop of ISS operation
- Collaborative framework has been established between Korea Aerospace Research Institute (KARI) and Korea Research Insititue of Bioscience & Biotechnology (KRIBB). Especially, the interests on CubeSat-based platform technology has been shared between the members of both institutes, seeking further collaboration in this regard with the aim to participate in Korean Lunar Exploration Project, of which missions were scheduled to happen in 2017 and 2020. In this period, the operation of ISS will be stopped as agreed between its participating nations. Bio-Science mission using CubeSat-based platform technology will be a viable option to replace Bio-Science mission using ISS modules, not depending on unreliable option of Chinese Space Station, Tiangong-2, of which operation is scheduled to happen in 2020.

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서(보고서 초록) ... 3
• 요 약 문 ... 4
• SUMMARY ... 6
• CONTENTS ... 9
• 목 차 ... 10
• 제1장 연구개발과제의 개요 ... 11
• 제1절. 연구개발의 필요성 : Microgravity (μG)-‘Enabled’ Space-Biotechnology ... 11
• 제2장 국내외 기술개발 현황 ... 12
• 제1절. μG 환경활용 바이오기술현황 ... 12
• 제2절. 국제우주정거장(ISS) 현황 및 전망 ... 13
• 제3장 연구개발수행 내용 및 결과 ... 14
• 제1절. 구조생물학연구를 위한 ISS μG-환경 단백질결정성장 ... 14
• 제2절. μG-모사장치 활용 3-D 세포배양 ... 18
• 제4장 목표달성도 및 관련분야에의 기여도 ... 24
• 제1절. 연구개발목표의 달성도 ... 24
• 제2절. 관련분야의 기술반전에의 기여도 ... 24
• 제5장 연구개발결과의 활용계획 ... 25
• 제6장 연구개발과정에서 수집한 우주 미션 정보 : 큐브위성 첨단기술 ... 26
• 제7장 연구시설‧장비 현황 : 구조생물학 연구를 위한 바이오 빔라인 ... 27
• 제8장 참고문헌 ... 28
• 끝페이지 ... 29