초록 ▼

◦ 자외선 흡수 물질을 분리하기 위하여 UV/Vis-spectrophotometer 및 HPLC를 이용한 자외선 흡수 MAAs 존재 미세조류를 확인하고 온도, 광량, 광주기, pH 및 엽록소 형광분석에 따른 표준배양조건 확립
◦ 미세조류 대량배양을 위한 5L, 20L 생물반응기 배양공정 및 고주파 처리에 의한 MAAs 함량을 높이는 배양 공정 확립
◦ 미세조류에서 MAAs 생합성 관련 유전자(NRPS, DHQS) 발현 확인 및 바이오 마커로서의 검증
◦ 미세조류 유래 자외선 흡수물질 추출·분리정제 방법확립
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◦ 자외선 흡수 물질을 분리하기 위하여 UV/Vis-spectrophotometer 및 HPLC를 이용한 자외선 흡수 MAAs 존재 미세조류를 확인하고 온도, 광량, 광주기, pH 및 엽록소 형광분석에 따른 표준배양조건 확립
◦ 미세조류 대량배양을 위한 5L, 20L 생물반응기 배양공정 및 고주파 처리에 의한 MAAs 함량을 높이는 배양 공정 확립
◦ 미세조류에서 MAAs 생합성 관련 유전자(NRPS, DHQS) 발현 확인 및 바이오 마커로서의 검증
◦ 미세조류 유래 자외선 흡수물질 추출·분리정제 방법확립
◦ MAAs 중 Porphyra, Shinorine 분리정제 후 소재 산업화 ◦ MAA 융합 펩타이드 신소재 개발 및 국제 CTFA 인허가
◦ 미세조류 형질전환체 Biomass 분석 및 MAAs 생합성 유전자 검증
◦ 미세조류 형질전환체 25톤 규모 대량배양 공정 확립 후 대량 생산
◦ 미세조류 유래 기능성 화장품 소재의 임상 평가를 통한 제품화 및 사업화

Abstract ▼

Ⅳ. R & D results
◦ Separation of UV-absorbing material
- 20 types of microalgae culture conditions established abroad
- 19 types of UV-absorbing MAAs present in the microalgae were confirmed using a UV / Vis-spectrophotometer and HPLC
- Standard culture conditions were established accord

Ⅳ. R & D results
◦ Separation of UV-absorbing material
- 20 types of microalgae culture conditions established abroad
- 19 types of UV-absorbing MAAs present in the microalgae were confirmed using a UV / Vis-spectrophotometer and HPLC
- Standard culture conditions were established according to the temperature, light intensity, photoperiod, pH, and chlorophyll fluorescence with respect to the outer Chlamydomonas and sp. 13 species confirmed as the UV-absorbing material
◦ Microalgae cultivation process established
- 5L, 20L bioreactor for the production of microalgae mass culture
- Process to establish to increase the content of MAAs in culture at 200 ~ 250 kHz high frequency process using microalgae bioreactor
◦ Explore utilization of microalgae as MAAs biosynthesis-related genes and biomarkers
- by high-frequency process the physical content of MAAs increased and the utilization of MAAs biosynthesis related to biomarker by NRPS, a 1.15-fold and 1.43-fold increased expression of genes DHQS attended
◦ UV-absorbing material extraction and purification method established
- Extraction, separation and purification of cultured species in 20L- Higher content of MAAs Chlamydomonas sp. of 249 mg Shinorine, secure Asterine Mycosporine glycine, in the 15 mg Palythine, 58 mg 59 mg of the 740 mg Porphyra 334.
◦ Development MAA purified fusion peptide separation Materials
- MAAs a type of Porphyra, Shinorine, Mycosporine-glycine, Palythine and Asterine as a cosmetic material safety cell, antioxidant, and anti-wrinkle effects identified
- Development of 12 types of MAAs-Peptide Synthesis derivatives
- As a cosmetic ingredient, an ultraviolet protection factor (SPF) 9 A secure and UV index (PFA) + clinical trials applied to the human body
- Completed 04 Articles for Cosmetics International Association license on MAA fusion peptide material
- Prototype production of 3000 sets of Gel mask pack
◦ Development MAA biosynthesis using genetic engineering techniques to promote bird
- Complete cloned into plant expression vector of the Glucose transporter Glut-1 gene
- Performing Glut-1-Agrobacterium mediated gene transformation using the transformation
- The Glut-1 gene transgenic microalgae Chlamydomonas hedleyi completed the selection element
◦ Transgenic microalgae Biomass analysis and verification MAAs biosynthesis genes
- Securing of Glut-1 transgenic microalgae Biomass and MAAs content analysis
- Securing, the Glut-1 (+) Growth curves and Biomass measurement results for the cancer condition state Glut-1 (-)
- Securing of Glut-1 (+) Glucose concentration and growth curve in accordance with the measurement result Biomass
◦ Transgenic microalgae cultivation process established for body mass
- Two types of strains for the Glut-1 gene transfection and mass culture microalgae
- 25000 Liters of mass culture produced by the selected strains
◦ Commercialization through the clinical evaluation of functional cosmetic materials derived from microalgae
- Standardization of building materials through the mass production system
- Completed 07 licensing of microalgae-derived material like MAAs to Cosmetics International Association
- Two prototypes (production of 5000 sets) (Newtone Daily Defence Sun Cream, Newtone Daily Defence Complete Control Essence) were developed using materials derived from microalgae
- As cosmetics trials, Human primary skin irritation trials completed
- Completion of clinical trials as a sunscreen cosmetics