초록 ▼

Ⅳ. 연구개발결과
○ 오이 흰가루병에서 에스필과 수산화동의 혼용 처리는 4 : 1 혼용 처리구에서 발병엽률 18.9%와 방제효과 69.9%, 8 : 1 혼용 처리구에서 발병엽률 18.1%와 방제효과 71.1%로 가장 효과적이었다. 토마토 흰가루병에서 에스필과 수산화동의 혼용 처리는 4 : 1 혼용 처리구에서 발병엽률 12.4%와 방제효과 85.3%, 8 : 1 혼용 처리구에서 발병엽률 14.3% 와 방제효과 83.0%로 가장 효과적이었다.
○ 에스필과 수산화동 혼용처리에 의한 오이와 토마토 흰가루병에 대한 방제 효과는 4

Ⅳ. 연구개발결과
○ 오이 흰가루병에서 에스필과 수산화동의 혼용 처리는 4 : 1 혼용 처리구에서 발병엽률 18.9%와 방제효과 69.9%, 8 : 1 혼용 처리구에서 발병엽률 18.1%와 방제효과 71.1%로 가장 효과적이었다. 토마토 흰가루병에서 에스필과 수산화동의 혼용 처리는 4 : 1 혼용 처리구에서 발병엽률 12.4%와 방제효과 85.3%, 8 : 1 혼용 처리구에서 발병엽률 14.3% 와 방제효과 83.0%로 가장 효과적이었다.
○ 에스필과 수산화동 혼용처리에 의한 오이와 토마토 흰가루병에 대한 방제 효과는 4 : 1 혹은 8 : 1 혼용 처리가 가장 효과적이었다.
○ 나방류 해충에 효과적인 곤충병원성 선충 Steinernema 속과 Heterorhabditis 속 선충, 곤충병원성 곰팡이 Metarhizium 속, Beauveria 속 미생물을 탐색 및 선발하였다.
○ 곤충병원성 선충을 이용하여 토마토 주요 해충인 왕담배나방에 대한 살충력과 포장 이용 기술을 개발하였다.
○ 시설원예작물에 피해를 주는 미소해충인 목화진딧물, 복숭아혹진딧물, 꽃노랑총채벌레, 점박이응애에 살충력이 우수한 식물추출 조성물과 에센셜오일을 선발하고 이용기술을 개발하였다.

Abstract ▼

This research was performed to examine the effects of mixed Espil and Copper hydroxide for on powdery mildew prevention on cucumber, tomato and pepper. On prevention effect for powdery mildew on cucumber, results revealed that mixed Espil and Copper hydroxide with a ratio of 4 : 1, disease incidence

This research was performed to examine the effects of mixed Espil and Copper hydroxide for on powdery mildew prevention on cucumber, tomato and pepper. On prevention effect for powdery mildew on cucumber, results revealed that mixed Espil and Copper hydroxide with a ratio of 4 : 1, disease incidence rate was 18.9% and prevention effect was 69.9%. Another treatment with a ratio of 8 : 1 showed a disease incidence rate of 18.1% and prevention effect of 71.1%, thus, showed great effectiveness. For powdery mildew on tomato, Espil and Copper hydroxide were mixed using the ratio 4 : 1, results showed a disease incidence rate of 12.4% and prevention effect of 85.3%. Treatment using the ratio of 8 : 1, results showed a disease incidence rate of 14.3% and prevention effect of 83.0%, thus, showed great effectiveness. For powdery mildew on red-pepper, Espil and Copper hydroxide were mixed using the ratio of 4 : 1 with results showed disease incidence rate of 17.7% and prevention effect of 83.0%. From the results, this treatment is the most effective with the lowest attack rate and highest prevention effect. Deducing from the study, it was found out that using mixed Espil and Copper hydroxide using the ratios 4 : 1 or 8 : 1 are the most effective method for powdery mildew prevention. Mixed ratio of 4 : 1 or 8 : 1 was most effective for preventing powdery mildew on cucumber and tomato, while the Espil and Copper hydroxide ratio of 4 : 1 was the most effective method for powdery mildew prevention on pepper. This research also was performed to examine the effects of use of mixed Espil and Copper hydroxide for pepper spotting disease and anthracnose prevention. On prevention effect of bacterial spotting disease, espil when used separately registered an attack rate of 59.6% and prevention effect of 28.9%. Mixed espil and copper hydroxide with a ratio of 4 : 1 showed the most effective treatment with a disease incidence rate on leaves of 20.0% and prevention effect of 76.1%. On the prevention effect for anthracnose, espil when used separately registered a disease incidence rate on leaves of 79.1% and prevention effect of 7.4%. Mixed espil and copper hydroxide with ratio of 4 : 1 showed the most effective treatment with disease incidence rate on leaves of 49.9% and prevention effect of 41.7%. Concluding from these results, it was found out that using mixed espil and copper hydroxide with ratio of 4 : 1 was more effective than using espil alone as method for pepper bacterial spotting disease and anthracnose prevention.
We isolated the entomopathogenic fungus, Beauveria spp. and Metarhizium spp. from Pieris rapae, Spodoptera litura and Plutella xylostella cavader collected in cultivation of Chinese cabbage and soybean in Suwon, Asan, Cheonan, Hyamyang and Jinju. Virulence assays against 2nd∼3rd larvae of P . xylostella, S. litura and P . rapae were conducted with various condidial suspensions from 105 to 107 conidia/ml of Beauveria spp. in laboratory conditions. Mortalities of P . xylostella, S. litura, and P . rapae were 83.3%, 80.0% and 93.3% in treatment density of 105 conidia/ml, 90.0%, 90.0% and 100% in treatment density of 106 conidia/ml, and 100%, 100% and 100% in treatment density of 107 conidia/ml, respectively. Entomopathogenic nematodes were surveyed from soil samples collected throughout the 147 locations using larvae of great wax moth, Galleria mellonella as food. The 25.9% of the total samples were positive for entomopathogenic nematodes with 21.8% containing Steinernema monticolum and 4.1% containing Heterorhabditis megidis.
Pathogenicities against young larvae of P. xylostella and P. rapae were over 90% with the dose of 10 Ijs per larva. With 0.1% addition of surfactants, i.e., PEG 200, PEG 400, Tween 20, and Tween 80, to vegetable oil, nematode productivity increased, showing final yields of 1.02×105, 1.10×105, 1.32×105, and 1.56×105 IJs/ml, respectively. Nematode productivity showed positive correlation with concentration of Tween 80. When 0.5% of Tween 80 was added to vegetable oil, nematode yield reached 1.98×105 IJs/ml. The highest yield of 2.28×105 IJs/ml was obtained at 240 h with an aeration rate of 3 vvm. Control test against Helicoverpa armigera with various concentrations of entomopathogenic nematode, Steinernema carpocapsae, was conducted in cherry tomato cultivation in Cheonan. Pathogenicity of S. carpocapsae gradually increased with increasing inoculation concentrations from 605,000,000 to 1,210,000,000 infective juveniles (Ijs) per 330㎡, and the control effect was similar to that of insecticide treatment with flubendiamide SP. Control effects of nematicidal composition comprising culture fluids of Myrothecium P10-008, 009, 057 and 218 were investigated on infective juveniles (Ijs) of Meloidogyne incognita in laboratory, showing that nematicidal effect of culture fluid Myrothecium P10-008 was over 80%. In addition, the densities of M. incognita reduced 88.5% at 100v/v solutions, 33.7% at 500v/v solutions, and 0% at 1,000v/v solutions of culture fluid of Myrothecium P10-008 in 96 days after treatments in cucumber greenhouses. Extracts of pyrethrin, pyrethrin+matrine, and nicotine showed insecticidal activities of 90.5%, 90.4% and 87.1% against Aphis gossypii at 1,000 times concentration in cucumber greenhouses. Moreover, extracts of pyrethrin, pyrethrin+matrine, and nicotine showed insecticidal activities of 57.5%, 91.0% and 86.4% against Tetranychus urticae at 1,000 times concentration in cucumber greenhouses. Insecticidal activities of the saponin extracts to Myzus persicae, A. gossypii , and T. urticae were 12.7∼82.5%, 15.0∼86.4% and 21.3∼80.7% at treatments of one, two and three times, respectively. We tested control effects of 100 essential oils against A. gossypii , M. persicae, Aulacorthum solani , T. urticae, Frankliniella occidentalis, and P. xylostella, showing that lavender oil, rosemary oil, and eucalyptus oil were effective in controlling for these pests.

목차 Contents

• 완결과제 최종보고서 ... 1
• 제 출 문 ... 2
• 요약문 ... 3
• SUMMARY ... 4
• 목 차 ... 6
• 제 1 장 서 론 ... 7
• 제 2 장 국내외 기술개발 현황 ... 8
• 제 3 장 연구개발수행 내용 및 결과 ... 9
• 제 4 장 연구개발목표 달성도 및 대외기여도 ... 32
• 1절 : 목표대비 달성도 ... 32
• 2절 : 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 33
• 제 5 장 연구개발결과의 활용계획 ... 33
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 33
• 제 7 장 기타 중요 변동사항 ... 33
• 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비 현황 ... 33
• 제 9 장 참고문헌 ... 34
• 끝페이지 ... 37