초록 ▼

Ⅳ. 연구개발결과
1. 한반도 유래 야생콩 및 우량 재배콩 16건의 유전체 재분석 결과를 생산하였다.
2. High-quality의 3.8 백만점의 SNP 및 0.5 백만점의 indel을 동정하여 이들 변이를 이용하여 다양한 집단유전적 분석을 수행하여 206개의 candidate domestication regions을 예측하였다.
3. 본 연구 결과는 2014년 4월 DNA Research (IF 5.00)의 게재하였다.
4. 2014년 추가적인 17건을 첨가하여 분석을 재수행하였다.
5. 지중해 원산인

Ⅳ. 연구개발결과
1. 한반도 유래 야생콩 및 우량 재배콩 16건의 유전체 재분석 결과를 생산하였다.
2. High-quality의 3.8 백만점의 SNP 및 0.5 백만점의 indel을 동정하여 이들 변이를 이용하여 다양한 집단유전적 분석을 수행하여 206개의 candidate domestication regions을 예측하였다.
3. 본 연구 결과는 2014년 4월 DNA Research (IF 5.00)의 게재하였다.
4. 2014년 추가적인 17건을 첨가하여 분석을 재수행하였다.
5. 지중해 원산인 M. truncatula를 UC-Davis의 Cook 교수팀과 국제협력을 통하여 고염토양에 적응/자생하는 40개의 유전형을 획득하였으며 평균 28X coverage로 NGS 기술을 이용한 재분석을 실시하였다.
6. 이를 바탕으로 집단유전학적인 분석을 수행하였으며 phylogeny 분석, 집단구조 분석, 교차이식 생리 분석, LD 분석, Tajimas’ D 분석, ROD 분석 등을 완료하였으며, 이를 통하여 염해적응과 유의하다고 판단되는 다수의 유전체 지역을 동정하였으며 내재해성에 관련된 중심 유전자 발굴 작업을 수행하였다.
7. 환경스트레스 반응/내재해성 유전자를 비교유전체적 접근 방식으로 발굴하고 이를 분자마커로 개발하였다.

Abstract ▼

Despite the intensive soybean [Glycine max (L.) Merrill] genome studies, the high chromosome number (20) of the soybean plant relative to many other major crops has hindered the development of a high-resolution genomewide genetic map derived from a single population. In this project, we constructed

Despite the intensive soybean [Glycine max (L.) Merrill] genome studies, the high chromosome number (20) of the soybean plant relative to many other major crops has hindered the development of a high-resolution genomewide genetic map derived from a single population. In this project, we constructed such a map, which was constructed in an F15 population derived from a cross between G. max and G. soja lines using indel polymorphisms detected via a G. soja genome resequencing. By targeting novel indel markers to marker-poor regions, all marker intervals were reduced to under 6 cM on a genome scale. Comparison of the Williams 82 soybean reference genome sequence and our genetic map indicated that marker orders of 26 regions were discrepant with each other. Then, we showed that these observed discordant regions are mostly errors in the Williams 82 assembly. Distributions of the recombination rates along the chromosomes were similar to those of other organisms. Given the even and dense distribution of markers, our genetic map can serve as a bridge between genomics research and breeding programs.
Despite the importance of soybean as a major crop, genome-wide variation and evolution of cultivated soybeans are largely unknown. In this project, we catalogued genome variation in an annual soybean population by high-depth resequencing of 10 cultivated and 6 wild accessions and obtained 3.87 million high-quality single-nucleotide polymorphisms (SNPs) after excluding the sites with missing data in any accession. Nuclear genome phylogeny supported a single origin for the cultivated soybeans. We identified 10-fold longer linkage disequilibrium (LD) in thewild soybean relative towild maizeandrice. Despite the small population size, the long LD and large SNP data allowed us to identify 206 candidate domestication regions with significantly lower diversity in the cultivated, but not in the wild, soybeans. Some of the genes in these candidate regions were associated with soybean homologues of canonical domestication genes. However, several examples, which are likely specific to soybean or eudicot crop plants, were also observed. Consequently, the variation data identified in this study should be valuable for breeding and for identifying agronomically important genes in soybeans. However, the long LD of wild soybeans may hinder pinpointing causal gene(s) in the candidate regions. In 2014, we updated this resequencing study by analyzing 19 cultivated and 19 wild soybeans that likely represent worldwide soybean populations. The results show that the number (2049) of candidata domestication genes were reduced to two thirds of that from the analysis of the previous small population.
Resequencing analysis is important and effective way of discovering trait-associated genomic regions that are linked to agronomically useful crop properties. To identify such genomic regions, a total of 40 Medicago truncatula genotypes, which is one of important legume model systems and physiologically well defined characters for salt tolerance, was used to establish the translational genomics approach for the purpose of applying its genomic information to the most important crop legume, soybean. The 40 M. truncatula accessions were samples in two Portugese geographical areas, Gilberto and Castro Marim where are its native habitats. Resequenced genomic data were mapped on Mt A17 reference genome and SNPs were called. Based on the SNP data, a diverse array of analyses were conducted including phylogeny/structure analysis, significant statistical analyses such as Fst, Tajima’s D, reduction of diversity (ROD) and association analaysis. As a result of those analyses, it was shown that eight genomic regions were predicted to be associated with genetic property of salt tolerance. Further detailed gene level analysis is currently under way. On the other hand, abiotic stress-responsive genes were screened and collected based on information using DB information and literatures, resulting in 1377 ABS-responsive gene set. Of these genes, 120 genes were used to develop the cross-species gene-based molecular markers.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요 약 문 ... 3
• SUMMARY ... 5
• 목차 ... 7
• 제 1 장 서 론 ... 8
• 제 2 장 국내외 기술개발 현황 ... 11
• 제 1 절 국내 연구 현황 ... 11
• 제 2 절 국외 연구 현황 ... 12
• 제 3 절 국내외 연구현황 비교 및 필요 연구 분야 ... 16
• 제 4 절 본 과제 착수 후 국내외 연구동향 변화 ... 17
• 제 3 장 연구개발수행 내용 및 결과 ... 20
• 제 1 절 연구내용 ... 20
• 제 2 절 연구결과 ... 25
• 제 4 장 연구개발목표 달성도 및 대외기여도 ... 63
• 제 1절 목표대비 대외 달성도 ... 63
• 제 2절 정량적 성과 ... 64
• 제 5 장 연구개발결과의 활용계획 ... 65
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 66
• 제 7 장 기타 중요 변동사항 ... 67
• 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비현황 ... 67
• 제 9 장 참고문헌 ... 68
• 끝페이지 ... 71