초록 ▼

Ⅳ. 연구개발결과
벼 화분 특이적 promoter에 결합된 GA생합성 저해용 유전자를 함유한 vector 28종으로부터 유래된 820개의 T0 식물체를 육성하여 조사하였다. T2세대에서 선발된 47계통들의 T3 progeny 280종 약 2800개체를 경북대학교 LMO 격리포장에 전개하였으며, 농업적 특성조사 수행하였다. 또한 12종의 Pollen-specific rice promoter와 표식유전자와 결합한 벡터를 제작하여 약 400개의 벼 형질전환 식물체 및 150개의 애기장대 형질전환체를 육성하여 화분 특이적 발현양상을

Ⅳ. 연구개발결과
벼 화분 특이적 promoter에 결합된 GA생합성 저해용 유전자를 함유한 vector 28종으로부터 유래된 820개의 T0 식물체를 육성하여 조사하였다. T2세대에서 선발된 47계통들의 T3 progeny 280종 약 2800개체를 경북대학교 LMO 격리포장에 전개하였으며, 농업적 특성조사 수행하였다. 또한 12종의 Pollen-specific rice promoter와 표식유전자와 결합한 벡터를 제작하여 약 400개의 벼 형질전환 식물체 및 150개의 애기장대 형질전환체를 육성하여 화분 특이적 발현양상을 규명하였으며, 9종 산업재산권 출원하여 현재 7건이 국내특허로 등록하였다. Activation tagging에 의해 co-segregation 확인된 돌연변이체 12종 이상을 확보하여 삽입부위 유전자를 동정하였으며, 화분발달관련 돌연변이체 7종로부터 변이유발 유전자들을 동전하고, 기본적인 특성분석 완료하였으며, 이들 중 돌연변이체 2종에 관하여 유전자 특성 및 기능분석 실험을 완료하여 6편의 SCI 국제학술지에 연구논문으로 게재하였다.

Abstract ▼

F₁ hybrid Male sterility is an important trait for crop breeding program based on heterosis. Recent advances in molecular researches have led to the identification of genes involved in plant reproductive development and understanding the molecular functions of rice male gametophyte includ

F₁ hybrid Male sterility is an important trait for crop breeding program based on heterosis. Recent advances in molecular researches have led to the identification of genes involved in plant reproductive development and understanding the molecular functions of rice male gametophyte including roles of phytohormones in reproduction process. F₁ hybrid production system which based on heterosis is a widely used method in crop breeding field. But in the rice breeding field, it is difficult to apply because of their strong self-pollination trait. It is strongly required a controllable male-sterility for F₁ hybrid seed production in rice.GA20ox (GA20ox1 and GA20ox3) and GA3ox (GA3ox1 and GA3ox2) are the important gene in GA biosynthesis pathway. For the control of GA biosynthesis at the floral organ, six anther specific promoter (Osc4, Osg6b, OsSCP1, OsSCP2, OsIPA) and three constitutive promoter (Maize ubiquitin, CaMV35S, OsActin1) were isolated and introduced RNAi destination vector. And also 4 GA-related gene RNAi fragment were inserted into RNAi destination vector by LR cloning. Also promoter-GUS:GFP fused control vector were constructed to verify promoter activity and used as a transformant control. As a result, constitutive promoter controlling GA20ox1 and GA3ox2 RNAi transformant showed 20% plant length decrease and also active GA content was decreased. And every RNAi transformant showed lower fertility than transformant control, promoter-GUS:GFP plant. OsGA3ox1 and OsGA20ox1 showed lowest fertility among RNAi transformants. And OsSCP1 and OsSCP2 promoter containing RNAi transformant showed lower fertility than any others. These results suggest that possibility of biotechnically induced MS rice. In this report, we provided various information on the genes required for key aspects of anther/pollen development and conventional methods for the production of hybrid seeds in rice. Finally, we presented the molecular approaches for the generation of male-sterile lines through the regulation of phytohormonal biosynthesis in reproductive organs.