초록 ▼

Ⅳ. 연구개발결과
(필요에 따라 제목을 달리할 수 있음)
○ Syngenta Crop Protection Ag (Basel, Switzerland) 와 2012년 5월 2일자로 3년간 research licencing과 non-exclusive commercial licencing 계약함.
○ 2013년, 2014년 4월 Syngenta (SBC(Syngenta Biotechnology China) ‘Agronomical Trait Team’ 연구팀와의 공동연구 협의
○ MPT1에 대한 해외 출원 및 등록

Ⅳ. 연구개발결과
(필요에 따라 제목을 달리할 수 있음)
○ Syngenta Crop Protection Ag (Basel, Switzerland) 와 2012년 5월 2일자로 3년간 research licencing과 non-exclusive commercial licencing 계약함.
○ 2013년, 2014년 4월 Syngenta (SBC(Syngenta Biotechnology China) ‘Agronomical Trait Team’ 연구팀와의 공동연구 협의
○ MPT1에 대한 해외 출원 및 등록
- 미국, 중국, 인도, 브라질에 특허 출원
- 2014년 7월 21일 MPT1 유전자에 대한 중국특허출원 no. 201080062158.1의 등록통지서
○ MPT1의 기능 연구
- MPT1은 직립형을 유도하는 positive transcription factor
- 벼 엽각 결정에 관여하는 BR과 옥신에 대한 MPT1의 역할
- MPT1 target 유전자 동정
- MPT1과의 결합 유전자 동정
○ Ubiquitinpromoter 와 POR (protochlorophyllide oxidoreductase)를 이용하여 구축된 MPT1 계통에 대한 생산력 포장 성적을 산출하여 활용성 검증
○ 3개 NAC의 ‘등숙 촉진’ 특성 분석

Abstract ▼

The objectives of the project is to isolate globally unique genes determining plant type and seed filling and to apply them to develop GM crops.
To isolate genes determining plant type and to evalulate agronomical utility of these genes, the following specific aims were established and experiment

The objectives of the project is to isolate globally unique genes determining plant type and seed filling and to apply them to develop GM crops.
To isolate genes determining plant type and to evalulate agronomical utility of these genes, the following specific aims were established and experiments were performed;
(1) isolation of new genes related to plant types and functional analysis
- microarray analysis of pulvinal tissues using mpt1 mutants
- isolation of putative MPT1 target genes carrying MPT1 binding motives
- analysis of MPT1 target genes using mutants and overexpressors of MPT1
- interaction study of MPT1 homologues, IDD12 and IDD13
- isolation of MPT1 interacting genes by yeast 2 hybrid assay
- BiFC (Bimolecular Fluorescence Complementation) assay with MPT1 interacting genes.
- analysis of plant types and graviresponse of MPT1 target or interacting genes.
(2) development of GM crops and analysis of field performance
- MPT1 trangenic lines using Ubiquitin promoter and POR (protochlorophyllide oxidoreductase) promoter
- evaluation of yield performance
To study grain filling, the following specific aims were established and experiments were performed;
- analysis of grain filling characters of three NAC transgenic lines
- analysis of transgenic lines harboring xylem-specific promoter BC6 expressing XNDL1 and 2 genes.
- evaluation of utility of transgenic lines by crossing to high yield rice varieties of Korea.