초록 ▼

Ⅳ. 연구개발결과
특수미인 눈큰흑찰 지용성분으로부터 황반변성 예방 효능이 있는 루테인을 분리 동정하였고, 최초로 유효성분 이상의 루테인이 함유되어 있음을 확인하였다. 카로티노이드 등 지용성 건강기능 성분을 한번에 분석할 수 있는 일괄 분석법을 정립하여 그 결과로 HPLC를 이용한 카로티노이드, 루테인, 비타민 E 등을 동시에 분석이 가능하게 되었다. 눈큰흑찰에 함유되어 있는 루테인은 244ug/100g로 이 함량은 일반미에 비해 50배 이상 많은 양이다. 베타카로틴의 경우는 눈큰흑찰이 25ug/g 함유하고 있었으나 일반미에는

Ⅳ. 연구개발결과
특수미인 눈큰흑찰 지용성분으로부터 황반변성 예방 효능이 있는 루테인을 분리 동정하였고, 최초로 유효성분 이상의 루테인이 함유되어 있음을 확인하였다. 카로티노이드 등 지용성 건강기능 성분을 한번에 분석할 수 있는 일괄 분석법을 정립하여 그 결과로 HPLC를 이용한 카로티노이드, 루테인, 비타민 E 등을 동시에 분석이 가능하게 되었다. 눈큰흑찰에 함유되어 있는 루테인은 244ug/100g로 이 함량은 일반미에 비해 50배 이상 많은 양이다. 베타카로틴의 경우는 눈큰흑찰이 25ug/g 함유하고 있었으나 일반미에는 동정되지 않았다. 또 다른 지용 성분으로 감마오리자놀이 동정되었는데 눈큰흑찰은 일반미에서 3개의 주요성분이 동정되는데 반해 8종 이상의 감마오리자놀로 구성되어 있음을 확인하였다.
미국인을 대상으로 한 임상시험에서는 눈큰흑찰 라이스칩(rice-chip) 복용에 의한 대사증후군 관련 마커 비교에서 통계적으로 유의한 차이는 관찰되지 않았으나 8주 복용 후 인슐린 반응 검정에서 눈큰흑찰이 유의하게 혈중 인슐린 함량을 감소시키는 효과가 있음을 확인하였다.

Abstract ▼

By becoming one of the hottest trends in consumers, LOHAS (Lifestyles of health and sustainability) gives us new world of ethical consumerism. Korean consumers require health rice cultivars and make purchase decisions with high criteria of healthy functions in rice foods. To select for these traits

By becoming one of the hottest trends in consumers, LOHAS (Lifestyles of health and sustainability) gives us new world of ethical consumerism. Korean consumers require health rice cultivars and make purchase decisions with high criteria of healthy functions in rice foods. To select for these traits rice breeders face other aspects of good grain quality and functional substances. We were considered some biological activities of lowering body weight, lowering plasma sugar contents and anti-oxidative activities. In addition, increase of nutritional and functional substances content were important breeding objectives.
‘Nunkeunhukchal’ is a black sticky rice cultivar with a giant embryo that was originally developed by anther culture and artificial crossing. Giant embryo rice was specialized in the component and content of free amino acid in the kernel of rice and the most significant of these was an increase of γ-aminobutyric acid (GABA) content.
Bioactive components in rice vary depending on the variety and growing condition.
Fat-soluble components such as γ-oryzanol, tocopherols, tocotrienols, carotenoids, and fatty acids were analyzed in brown, sugary brown, red, and black rice varieties using established high-performance liquid chromatography (HPLC) and GC methodologies. In addition, these colored rice varieties were further analyzed using a high-resolution liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) (LTQ-Orbitrap XL) to identify the [M-H]- ions of γ-oryzanol. The highest content of tocopherols (a-, 1.5; c-, 0.5 mg/100 g) and carotenoids (lutein 244; trans-b carotene 25 LG/100 g) were observed in black rice; tocotrienols (a-, 0.07; c-, 0.14 mg/100 g) in red rice, and γ-oryzanol (115 mg/100 g) in sugary brown rice. In all colored rice varieties, the major fatty acids were palmitic (16:0), oleic (18:1n-9), and linoleic (18:2n-6) acids. When the γ-oryzanol components were further analyzed by LC-MS/MS, 3, 10, 8, and 8 triterpene alcohols or sterol ferulates were identified in brown, sugary brown, red, and black rice varieties, respectively. Such structural identification can lead to the elucidation of biological function of each component at the molecular level. Consumption of colored rice rich in beneficial bioactive compounds may be a useful dietary strategy for achieving optimal health.
To determine the beneficial effect of brown rice and its bioactive constituents on metabolic risk factors in overweight/obese people with central adiposity. A randomized, placebo controlled, 2-way crossover pilot feasibility study was conducted to determine the effect of brown rice on metabolic risk factors in 9 subjects with MetS. Qualified subjects with metabolic syndrome (≥50 y, post-menopausal women and men) were randomized to receive either brown rice cakes or white rice cakes (100 g/d) for 5 wk with a 2-wk washout between the crossover. Nine eligible subjects were enrolled in the study. The effect of brown rice on blood pressure, glucoregulation and lipid profile was null. For one subject (1198-024) plasma insulin levels dropped drastically after the brown rice phase (21.1 U/mL) compared to baseline (58.9 U/mL). . These improvements suggest that brown rice may have the potential to modestly reduce several important risk factors for diabetes and cardiovascular disease. A follow-up study with increased sample size is necessary to provide more conclusive results. For future crossover studies, a baseline and washout period of 4 weeks is recommended to accurately measure changes in blood lipids.