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Kafe 바로가기주관연구기관 | 서울대학교 Seoul National University |
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보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 | 한국어 |
발행년월 | 2015-02 |
주관부처 | 농촌진흥청 Rural Development Administration(RDA) |
등록번호 | TRKO201500010557 |
DB 구축일자 | 2015-07-11 |
Ⅳ. 연구개발결과
- 고온 스트레스 신호전달 반응을 관장하는 주요 유전자들(OsMADS3 과 Invertase)의 11개의 품종 간 특이적 발현차이를 확인하였다. 세포질 특이적인 Novel Soybean BCH(β-Carotene Hydroxylase) 유전자의 기능 구명을 통해 광저해 및 스트레스(고온) 저항성 벼에 활용 가능성을 제시하였다. EMS mutagenesis에 의한 고온저항성 상실 돌연변이 벼 27계통을 선발 및 고온 저항성을 평가하였다. 또한 벼의 화분에서 내서성 및 내냉성에 관여하여 특이하게 발현하는 137개
Ⅳ. 연구개발결과
- 고온 스트레스 신호전달 반응을 관장하는 주요 유전자들(OsMADS3 과 Invertase)의 11개의 품종 간 특이적 발현차이를 확인하였다. 세포질 특이적인 Novel Soybean BCH(β-Carotene Hydroxylase) 유전자의 기능 구명을 통해 광저해 및 스트레스(고온) 저항성 벼에 활용 가능성을 제시하였다. EMS mutagenesis에 의한 고온저항성 상실 돌연변이 벼 27계통을 선발 및 고온 저항성을 평가하였다. 또한 벼의 화분에서 내서성 및 내냉성에 관여하여 특이하게 발현하는 137개의 단백질체를 동정 하였다.
- 배추의 276종 AP2/EREBP 유전자 및 10종 CBF/DREB1 유전자를 발굴하였고, 인삼의 314종 AP2/EREBP 유전자 및 22종 CBF/DREB 유전자를 발굴하였다. 다양한 환경스트레스(저온, 건조, 염, 고온)에 대한 강한 반응성 및 생육기간동안의 다양한 발현패턴을 확인하였다. 배추 및 인삼 CBF/DREB 유전자와 애기장대 상동유전자간의 micro-synteny를 확인하였다. 인삼 AP2/EREBP 및 CBF/DREB 유전자의 프로모터상에 존재하는 환경스트레스 반응성 cis-acting element를 확인하였다. 인삼 6종 CBF/DREB 유전자와co-expression하는 7종의 HSF 유전자를 확인하였다.
- 열대지방에서 특이적으로 발현하는 4개의 이삭길이 관련 QTL을 발굴하였는데, 특히 1번과 11번의 QTL의 경우, 물과 인산 부족에 특이적이며, 자포니카인 TR22183에서유래하였다. 11번은 건기와 우기에 관계없이 발현하는 QTL로서 활용가치가 높을 것으로 사료된다. 또, 각 QTL에 연관된 고효율 분자육종을 위한 시스템에 적합한 분자마커들을 개발하였다. 이에 대한 세밀지도를 작성하고 관련 후보유전자를 동정하기 위해,양친인 다산벼와 TR22183의 전장게놈분석을 하여 맵핑하고 QTL 부위를 검토하여 후보유전자를 다수 동정하였다. 발굴된 분자마커들을 활용하여 QTL이 함유된 다산벼형TR22183함유 계통 근동질 집단들을 개발하였다. 특히, 기발표된 우수 QTL인 Pup1을 함유한 다산벼와 TR22183 유래 계통을 개발하였다. 이는 초기 건조 조건에서의 뿌리활착에도 도움을 줄 것으로 보인다.
III. Results summary of the research
Allele mining for genes conferring dual abiotic stresses (heat and cold) tolerance using transcriptome analyses in rice
In this study, we conducted global gene expression profiling under heat stress condition. The result showed that the expression pattern o
III. Results summary of the research
Allele mining for genes conferring dual abiotic stresses (heat and cold) tolerance using transcriptome analyses in rice
In this study, we conducted global gene expression profiling under heat stress condition. The result showed that the expression pattern of some genes such as OsMADS3 and invertase which are known as regulating ROS metabolism were significantly different with control and indicated the function of these genes and the metabolism of ROS pathway are closely related to heat stress tolerance. In addition, β-carotene hydroxylase (BCH) is known as photo-inhibition and drought stress tolerance related factors as well as catalytic enzyme to synthase β-carotene in plants. BCH and its isomers might increase the tolerant level by catalyzing the synthesis of ABA hormone under stress conditions in plant.
Selection of the heat insensitive mutant in rice
To investigate the candidate genes conferring heat stress tolerance, seeds of Giza 178 (heat stress tolerant variety) were subjected to ethyl-methane-sulfonate (EMS) mutagenesis and several mutant lines showing heat insensitive phenotype were selected in M2 generation; a total of 27 mutant lines were selected and evaluated for physiological and morphological responses to heat stress.
Proteomic approaches to address dual abiotic stresses (heat and cold) tolerance in rice
Pollen is generally known as an important factor for seed setting ratio during pollination and fertilization, therefore, we analyzed the changes of pollen proteins in response to dual abiotic stresses (heat and cold) in protein level using shotgun proteomic approach. We found a total of 137 proteins that showing induced expression by heat stress, and the expression of several proteins were specifically regulated depends on the temperature (such as LOC_Os05g44340.1 protein for low temperature and LOC_Os02g52150.2 protein for high temperature).
Characterization of CBF/DREB family genes using genomic and transcriptomic databases for B. rapa and P . ginseng.
A total of 286 AP2/EREBP family genes were identified using genomic database for B. rapa. Of these, expression profiling of ten CBF/DREB1 family genes were further analyzed in response to abiotic stresses. Additionally, a total of 314 AP2/EREBP family genes (including 11 CBF/DREB1 family genes and 11 DREB2 family genes) were identified using genomic database for P. ginseng. Expression profiling for these identified P. ginseng genes also analyzed in response to abiotic stresses. Of the identified CBF/DREB family genes in P. ginseng, we found several cis-acting elements that are known to play an important role in response to abiotic stresses. In addition, six CBF/DREB family genes showed similar gene regulation patters with that of seven genes encoding heat stress transcription factors (HSF) in P. ginseng. The Comparative analysis revealed the micro-synteny of CBF/DREB1 loci between B. rapa and P. ginseng based on the Arabidopsis homologous genes.
QTLs analysis for panicle length under dual abiotic stresses (heat stress and P deficiency) conditions in rice
A total of 137 F15 recombinant inbred lines(RILs) that derived from across between Dasanbyeo(tongil- or temperate indica-type) and TR22183 (temperate japonica-type) were evaluated for 16 agronomic traits together with parent varieties. This evaluation was carried out at IRRI under both different P-levels and irrigated conditions during dry and wet seasons. The QTL analysis was conducted using composite interval mapping. Of the 16 targeted agronomic traits, panicle length showed significant correlation to enhanced yield under low P and water deficit conditions.
The effects of low P and water deficit were found to be significant in both wet and dry seasons. In terms of panicle length, both parent varieties and RILs showed different levels of sensitivities to low P and water deficit. The identified QTLs for panicle length in response to low P and rainfed conditions were found in chromosomes 1 (pl01), 2(pl02), 9 (pl09), and 11 (pl11). In addition, we found a total of 20 QTLs that related to the yield. Interestingly, we found that these QTLs are collocated with the identified four QTLs for panicle length. A significant correlation between yield components and panicle length was also observed, especially in the RILs grown under low P and rainfed conditions. The RILs contain a combination of Dasanbyeo allele at pl02 and TR22183 allele at pl11 showed relatively longer panicle length under low P and rainfed conditions in both dry and wet seasons.
QTL mapping and identification of candidate genes for longer panicle length under multifactorial abiotic stresses in rice
For the identified QTLs, we used fine-mapping strategy. To design the molecular markers for the fine-mapping strategy and the identification of candidate genes, we compared the whole-genome sequences of two different varieties. Several candidate genes that located in the identified QTLs and previously identified under low P and water deficit conditions were used for designing molecular markers, gene expression profiling, and fine-mapping. Of these, some of candidate genes located in the identified four QTLs were also reported in other studies as well under low P and water deficit conditions. Now, transcriptome sequencing is ongoing to identify the best candidate genes on each identified QTLs. A major QTL for P-deficiency tolerance, which located on chromosome 12 and originated from Kasalath (aus-type) has been successfully introgressed into two parent varieties – Dasan and TR22183.
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