초록

Ⅳ. 연구개발결과
정제봉독 유효성분의 안정성 확인 및 Optical coherence tomography(OCT)을 이용하여 피부침투력 정도를 확인하고 활막세포에서의 항염증작용, 파골세포 형성의 억제,연골세포의 연골 분해효소 억제능 확인정제봉독의 섬유화 억제능을 구멍하고자 한다.

Abstract ▼

Bee venom is a natural ingredient produced by the honey bee (Apis mellifera), and has been widely used in China, Korea and Japan as a traditional medicine for various diseases such as arthritis, rheumatism, and skin diseases However, the regulation of the underlying molecular mechanisms of the anti-

Bee venom is a natural ingredient produced by the honey bee (Apis mellifera), and has been widely used in China, Korea and Japan as a traditional medicine for various diseases such as arthritis, rheumatism, and skin diseases However, the regulation of the underlying molecular mechanisms of the anti-arthritis by bee venom and its major peptides is largely unknown. In this study, we investigated 1) effects of melittin on the migration of RA synoviocyte cells.
Also, we investigated the potential roles of a melittin, in the activation of osteoclast expression through the RANKL-induced MAPK signaling pathway in RAW 264.7 cells. 2) the potential molecular mechanisms underlying the anti-arthritis effect of bee venom and its major peptides, melittin and apamin, in tumor necrosis factor-α (TNF-α) responsive C57BL/6 mice chondrocyte cells. 3) skin permeation of bee venom experiment using Franz diffusion cell. 4) Optical Coherence Tomography (OCT) technology was used for this study to visualize the PBV delivery inbiological stillborn piglet skin tissues.
These data suggest that Honey bee (Apis mellifera) venom and its major polypeptide, melittin, have been shown to exert anti-inflammatory effects through suppression of the cell migration and reduction of osteoclastogenesis.
Also, melittin suppresses TNF-α-stimulated decrease of type II collagen expression by the inhibiting MMP-1 and MMP-8 through regulation of the NF-kB and AP-1 pathway and provision of a novel role for melittin in anti-arthritis action. These results suggested that melittin can be used as a clinical drug for the treatment of inflammation associated with osteoarthritis diseases.