보고서 정보
주관연구기관 |
국립농업과학원 National Institute of Agricultural Sciences |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2016-02 |
과제시작연도 |
2015 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
과제관리전문기관 |
국립농업과학원 National Institute of Agricultural Sciences |
등록번호 |
TRKO201600003079 |
과제고유번호 |
1395039853 |
사업명 |
농업기초기반연구 |
DB 구축일자 |
2016-06-25
|
DOI |
https://doi.org/10.23000/TRKO201600003079 |
초록
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Ⅳ. 연구개발결과
○ 주요 해충분류군 채집 및 유전자원 확보
○ 새로운 해충 종 동정기술 개발(고들빼기총채벌레 등 4종)
○ 분자마커를 이용한 해충 동정기술 개발(미국선녀벌레 등 43종)
○ 지역별 멸구류, 나방류 유전자원 확보
○ PCR을 통한 벼멸구 동정기법 확립
○ 이화명나방, 멸강나방 초위성체 마커 탐색, 선발
○ 초위성체 마커를 이용한 나방류 아개체군 유전 구조 분석
○ 대형노린재류 유전자원 채집 및 GIS layer 수집
○ 페로몬 트랩을 이용한 대형 노린재류 발생 밀도 변
Ⅳ. 연구개발결과
○ 주요 해충분류군 채집 및 유전자원 확보
○ 새로운 해충 종 동정기술 개발(고들빼기총채벌레 등 4종)
○ 분자마커를 이용한 해충 동정기술 개발(미국선녀벌레 등 43종)
○ 지역별 멸구류, 나방류 유전자원 확보
○ PCR을 통한 벼멸구 동정기법 확립
○ 이화명나방, 멸강나방 초위성체 마커 탐색, 선발
○ 초위성체 마커를 이용한 나방류 아개체군 유전 구조 분석
○ 대형노린재류 유전자원 채집 및 GIS layer 수집
○ 페로몬 트랩을 이용한 대형 노린재류 발생 밀도 변동 조사
○ 썩덩나무노린재 집합페로몬 루어 효과 검정
○ 톱다리개미허리노린재 공간 분포 분석
○ 썩덩나무노린재 세대수 조사 분석 및 기주에 따른 발육 조사
○ Harmonic radar를 이용한 썩덩나무노린재 탐색
○ 대형 노린재류 초위성체 마커 탐색, 선발 및 집단유전 연구
Abstract
▼
This study was conducted to develop the biological techniques for identification and management of insect pests with molecular markers, composing of three projects.
1) The recent increase of invasive or sporadic insect pests demands an alternative tools for fast and accurate identification. Along
This study was conducted to develop the biological techniques for identification and management of insect pests with molecular markers, composing of three projects.
1) The recent increase of invasive or sporadic insect pests demands an alternative tools for fast and accurate identification. Along with morphological methods, we try to investigate the possibility of molecular identification with mtDNA COI gene as molecular marker. Major taxa dealt in this study was Flatidae, Tingidae, Aleyrodidae, and Thripidae for invasive pests, and Ricaniidae and Acrididae for sporadic pests. Except of Thripidae in Thysanoptera and Acrididae in Orthoptera, all taxa belong to Hemiptera. The results shows that it is possible to identify 43 species with COI maker, consisting with morphological results.Species number of each family determined with nucleotide sequences was three in Flatidae with Metcalfa pruinosa (invasive), five in Tingidae with Corythucha marmorata (invasive),four in Aleyrodidae with Mediterranean genetic group of Bemisia tabaci (invasive), eleven in Thripidae with Frankliniella occidentalis and Thrips palmi (invasive), five in Ricaniidae with Ricania sp. (sporadic), and ten in Acrididae with Locusta migratoria (sporadic). During the study, we found that Caliothrips tongi in Thripidae as an unrecorded thrips severely occurred on leaves of Lactuca indica (Compositae) in Korea, and that Bemisia tabaci and Trialeurodes vaporariorum in Aleyrodidae was easily distinguished by lateral margins of ventral abdominal wax plates.
2) This study was conducted to increase the knowledge of the genetic diversity of several insect pest (planthoppers and moth) populations using genetic markers. We developed molecular-based identification technique of major planthppers using species specific PCR primer. In order to clarify there relationships among different populations of Chilo suppressalis in South Korea, 118 COI sequences from 118 samples collected from 26 location at South Korea. A total of 19 haplotypes was detected from the 118 COI sequences, and two haplotypes, hap-2 and 16 were major in South Korea. We investigated the population genetic structure of Chilo suppressalis among eighteen locations across South Korea using eight microsatellite markers developed by Liu et al. (2009). The number of alleles per locus ranged from 5 to 48 with an average of 21.38 alleles per locus. The mean observed heterozygosity of the eighteen population ranged from 0.034 to 0.960 and expected heterozygosity ranged from 0.063 to 0.908. Also, we investigated the population genetic structure of Psuedaletia separata among seven population caught in South Korea using six microsatellite markers. The number of alleles per locus ranged from 17 to 25 with an average of 21.67 alleles per locus. The mean observed heterozygosity of the seven population ranged from 0.148 to 0.969 and expected heterozygosity ranged from 0.203 to 0.911. By using developed primer sets of microsatellite, population genetic studies could be better understand the genetic structure of major insect pest populations.
3) This study was conducted to develop a system for predicting spatial distribution of stink bugs using geospatial analysis, sensing technology and population genetics. We collected Riptortus pedestris specimen at thirty locations in Republic of Korea and Halyomorpha halys specimens at forty-five locations in Republic of Korea and United State of America. Several GIS layers were collected to analysis relationship between environment factors and habitats of stink bug. Seasonal occurrence was investigated R. pedestris and H. halys using aggregation pheromone trap in Republic of Korea and United State of America. We tried to assess field responses of newly made aggregation pheromone lure developed by West Virginia University team. We conducted to characterize the patterns of population dynamics and spatial distribution of R. pedestris to predict its seasonal movement in landscape by SADIE. We examined the host suitability and possible generation time of H. halys in West Virginia. We conducted to confirm and establish the efficacy of the harmonic radar in detecting adult H. halys in various agricultural landscape. There was no significant difference in the search time needed to locate tagged adults. In general, it took less than 2min to detect and recover H. halys. The success rates of locating radar-tagged H. halys were also compared among different locations within mature fruit trees. There was no significant difference in the success rates between the inner third (87%) and the outer third of the host tree canopy (100%). However, a significantly longer period of time was required to locate H. halys in the inner canopy (372 s ± 95 SE) compared with the outer canopy (148s ± 39 SE). R. pedestris (Fabricius) has caused severe agricultural damage in Asia regions including Korea, Japan, and China. To clarify genetic relationships among different populations of R. pedestris in Korea, 294 COI sequences were analyzed from 294 samples collected in 42 local regions. A total of 36 haplotypes were detected from the 294 COI sequences, and one haplotype, hap-2, was observed from 225 COI sequences. The genetic distances among the 42 local populations were ranged from 0.00% to 1.50%, and the MJ networks of the 36 COI haplotypes revealed that R. pedestris has spread in Korea. Especially, genetic variations were actively detected in four groups, III, IV, V, and VI, among nine geographically defied groups. In this study, we discussed the genetic variation, relationship, and expansion of R. pedestris in Korea. We studied to evaluate genetic diversities of H. halys and the spread of its COI haplotypes, 79 specimens of Korea and 10 specimens of United State of America were newly analyzed and compared with previous reported 735 COI sequences of H. halys. A total of 45 haplotypes were detected from 10 countries, of which 16 haplotypes (H34–H49) and two haplotypes (H50 and H51) were newly detected from Korea and USA, respectively. This study represents a comprehensive analysis of H. halys populations in Korea and provides useful information of the Asian populations in order to understand better the global distribution of H. halys haplotypes. We investigated the population genetic structures of R. pedestirs among sixteen populations in Republic of Korea using four microsatellite markers developed by NGS. The number of alleles per locus ranged from 7 to 26 with an average of 14.75 alleles per locus. Also, we investigated the population genetic structures of H. halys among eighteen populations in Republic of Korea and United State of America using four microsatellite markers developed by NGS. The number of alleles per locus ranged from 7 to 17 with an average of 12.25 alleles per locus. By using developed primer sets of microsatellite, population genetic studies could be better understand the migration routes and the pylogenetic relationships between the stink bug populations.
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