초록 ▼

Ⅳ. 연구개발결과
국립원예특작과학원 직무육성품종 및 민간육성 심비디움, 팔레놉시스 신품종 대량생산을 진행하였다. 심비디움은 경기지역, 충청지역, 제주지역 종묘 생산팀으로부터 1,413,300주를 생산하여 생산목표 1,230,000주 대비 114.9% 초과달성하였다. 팔레놉시스는 배양방법에 따라 PLB증식 방법과, 부정아증식 등 증식방법별로 1,563,825주를 생산하여 생산목표 1,190,000주 대비 131.4% 초과달성하였다. 총 심비디움 및 팔레놉시스 국산품종 2,977,125주를 생산하였다.
생산된 종묘를 이용한

Ⅳ. 연구개발결과
국립원예특작과학원 직무육성품종 및 민간육성 심비디움, 팔레놉시스 신품종 대량생산을 진행하였다. 심비디움은 경기지역, 충청지역, 제주지역 종묘 생산팀으로부터 1,413,300주를 생산하여 생산목표 1,230,000주 대비 114.9% 초과달성하였다. 팔레놉시스는 배양방법에 따라 PLB증식 방법과, 부정아증식 등 증식방법별로 1,563,825주를 생산하여 생산목표 1,190,000주 대비 131.4% 초과달성하였다. 총 심비디움 및 팔레놉시스 국산품종 2,977,125주를 생산하였다.
생산된 종묘를 이용한 주산지 시범재배는 심비디움, 팔레놉시스 재배농가가 집중되어있는 경기지역과 충청지역을 선정하였고, 심비디움 절화수출은 제주지역, 팔레놉시스 주산지 시범재배는 경남지역을 선정하였다. 팔레놉시스, 심비디움 경기지역 시범재배는 1,010,904주, 충청지역 1,009,080주를 시범재배 하였고, 경남지역은 팔레놉시스 128,000주, 제주지역은 심비디움 절화수출을 위한 품종선발을 목적으로 39,700주를 시범재배 하였다. 절화수출을 위한 시범재배 뿐 아니라, 유묘수출 시장 확보를 위하여 팔레놉시스 유묘 20,000주를 미국 Dash Dream Inc에 시범 수출 하였고, 지산 미국법인에 심비디움 640,300주를 수출하였으며, 동서양교잡 심비디움 신품종 82,400주를 대만에 수출하였다.
절화 및 분화의 수출시장 확대를 위한 수출국 다변화를 위한 해외시장 정보분석 및 시장반응조사를 실시하였다. 제주지역농가로부터 국내육성 신품종 수출사업을 진행하여 일본에 FOD 8,653달러를 수출하였으며, 심비디움 전체 수출량 197,459중 14,096주를 수출해 국산품종 비중이 7.1%를 점유할 수 있었다. 더 낳아가 수출확대를 위하여 베트남 수출 시장조사 및 시범수출을 진행하였다.
종묘생산의 현장애로 기술을 개발하기 위하여 심비디움, 팔레놉시스 고효율, 균일종묘 생산기술연구를 진행하였다. 팔레놉시스 클로묘 대량생산에 적합한 절편체(줄기) 및 배지내 첨가물(50 ml·L^-1 coconut water, 0.54 g·L^-1 activated charcoal, 생장조절물질(2.0 mg·L^-1 TDZ), LED 광원(PLB 유도: Red 광원, PLB 증식, 신초 분화: Green 광원) 선발하였다. 심비디움 PLB 증식 및 소식물체 형성에 효과적인 배지(2.54 g·L^-1 Hyponex), 탄소원(2% sucrose), 활성탄(0.27 g·L^-1), LED 광원(PLB 증식: Red 광원, 소식물체 생육: 형광등) 선발하였다.

Abstract ▼

National Institute of Horticultural and Herbal Science (NIHHS) and Private breeding team breeded new varieties including Cymbidium and Phalaenopsis made a mass production of as its job cultivation and domestic cultivation variety. Cultivar Production Team in Gyeonggi, Chunngcheon and Jeju produced 1

National Institute of Horticultural and Herbal Science (NIHHS) and Private breeding team breeded new varieties including Cymbidium and Phalaenopsis made a mass production of as its job cultivation and domestic cultivation variety. Cultivar Production Team in Gyeonggi, Chunngcheon and Jeju produced 1,413,300 Cymbidium seedlings, which is 114.9% of the target production volume of 1,230,000. In addition, 1,563,825 Phalaenopsis were produced using cultivation methods of PLB cultivation, and adventitious seedling, which is 131.4% of the target (1,190,000). In total, 2,977,125 domestic cymbidium and phalaenopsis seedlings were produced.
Gyeonggi and Chungcheon regions were selected as the places for cultivation of the cymbidium and phalaenopsis as there were many farmers for the varieties. Jeju was selected as place for the export of cut flower of cymbidium while Gyeongnam area was selected as the main cultivation place for phalaenopsis. For the demonstration cultivation of cymbidium and phalaenopsis, 1,010,904 seedlings were cultivated in Gyeonggi, 1,009,089 in Chungcheon with 128,000 seedlings of phalaenopsis cultivated in Gyeongnam. In addition, 39,700 seedlings of cymbidium were cultivated for the purpose of selection of varieties for the export of cut flower of cymbidium. In addition to the demonstration cultivation for export of cut flower, 20,000 seedlings of Phalaenopsis were exported to Dash Dream Inc in US and 640,300 seedlings of cymbidium were exported to US branch of Jisan Inc. for the enlargement of the market size for the young seedlings. Also, 82,400 seedlings of the new variety of cymbidium, which is the hybrid of Eastern and western varieties, were exported to Taiwan.
For the expansion of export market for cut flowers and its diversification, the research on the overseas markets and market responses was conducted. According to the export project of new variety produced in Jeju, the export was made by farmers in Jeju to Japan with the amount of 8,653 FOD dollars and the domestic variety could take up 7.1% with the export of 14,096 seedlings among the total export of cymbidium of 196,459 seedlings. Furthermore, for the expansion of the export, the research on the Vietnam market and the demonstration export was conducted.
In order to investigate the effect of explants and plant growth regulators on protocorm-like body(PLB) induction and plant regeneration for the mass production system of domestic line Phalaenopsis (KV600 x 07PN50, 11-16) through tissue culture. We were selected the optimal explant and tested the effects of coconut water, activated charcoal and cytokinins on PLBs multiplication and regenerated plantlets for the mass production system of Phalaenopsis. The stem segment was the optimal explants among the leaves, shoot tips and induced in vitro PLBs. The highest frequency of PLB induction was obtained when stem segments were cultured on 1/2MS (Murashige & Skoog, 1962) supplemented with 50 ml·L^-1 coconut water. The survival rate of PLB and regenerated plants was effective on Hyponex medium with 0.54 g·L^-1 activated charcoal. Shoot and multishoot formation derived PLBs growth on Hyponex medium with TDZ was promoted among added BA, kinetin and 2iP. Especially, all normal plants were regenerated on Hyponex mediun with 2.0 mg·L^-1 TDZ.
Effect of light emitting diode(LED) lightings on PLB induction, multiplication and mass production of plantlets, stem segments from in vitro seedlings of Phalaenopsis domestic line (KV600×07PN50, 11-16) were cultured on 1/2MS with 0.5 mg·L^-1 TDZ added 50 mg·L^-1 coconut water or Hyponex media in the growth chamber contained various LED lights. The rate of PLBs induction and multiplication were highest under the green light as 5.4, 17.0, respectively, and number of regenerated plantlets were greatest as 5.8 after sixty days of culture. All plants were grew under various lighting sources of LEDs (red, blue, red+blue, green and white) as well as fluorescent light. The rate of growth were differed according to the lightened kinds of LED and developmental stage of in vitro regenerated plantlets. After sixty days of culture, the number of leaves (blue, 3.0), the length of leaves (red, 0.8 cm)and the width of leaves (white, 0.6 cm) were effectively observed compare to the other lights in plantlets of shoot length 1.0-1.5 cm. However, there is no significantly differences between the red and white lights. The red and blue mixed light were better than other lights in root development and additional propagated multiplication of PLBs. Also the shoot length (4.4 cm) and the number of leaves (4.9) were obtained in white and blue light, respectively, from the plantlets of shoot length 1.6-2.0 cm. The green light was effective in multiple shoot differentiation (2.1) and the fluorescent light was heavy (4.6 g) in fresh weight. On the other hand, the SPAD values in the leaves were greatly different between red+blue mixed light (33.4) and green light (50.9). These plants acclimatized in the growth chamber or green house.
To establish the mass production system of PLBs and regenerated plantlets, PLBs derived from apical meristem of in vitro seedlings in domestic Cymbidium cv. Green Ball were cultured on Hyponex (6.5:6:19) media. We investigated the effect of optimal concentration of media, carbon sources, activated charcoal and LEDs on the PLB production and plantlet regeneration via organogenesis. We first treated 1.27, 2.54, 3.81 g·L^-1 of Hyponex and next added 10, 20, 30 g·L^-1 of sucrose, sorbitol, maltose and 0.27, 0.54, 0.81 g·L^-1 of activated charcoal to the Hyponex media during 50 days of culture. The greatest number of PLB (3.9) produced on 2.54 g·L^-1 of Hyponex medium. PLB and shoots were vigorously multiplied on 2.54 g·L^-1 of Hyponex with 2% concentration of sorbitol. Also 0.27 g·L^-1 was the optimal concentration of activated charcoal for the high efficiency survival rate and proliferation of PLB and plantlets. The other hand, in order to investigate the effect of light emitting diode (LED) lightings on PLB multiplication and mass production of plantlets, 0.2-0.3 cm of PLBs were cultured on 2.54 g·L^-1 Hyponex media with 2% sucrose and 0.27 g·L^-1 activated charcoal in the growth chamber contained various LED lights. The PLB multiplication was highest under the red light as 6.3 and number of differentiated shoots were greatest as 3.6 after fifty days of culture.
2 stage of regenerated plantlets (2.5 and 2.5-3 cm plant height) were grew under various lighting sources of LEDs (red, blue, red+blue, green and white) as well as fluorescent lamp. The rate of growth were differed according to the lightened kinds of LED and developmental stage of in vitro regenerated plantlets. After two hundred and ten days of culture, the plant height (12.7 cm), the number of leaves (10.1), the length of leaves (10.4 cm), the width of leaves (5.9 mm), the number of roots (6.1) and the number of multiple shoots (5.8) were the most effective under the fluorescent lamp. However, green light was more longer than other light sources of the length of roots (4.9 cm).