보고서 정보
주관연구기관 |
국립축산과학원 National Institute of Animal Science |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 |
한국어
|
발행년월 | 2016-02 |
과제시작연도 |
2015 |
주관부처 |
농촌진흥청 Rural Development Administration(RDA) |
등록번호 |
TRKO201600003185 |
과제고유번호 |
1395040854 |
사업명 |
축산시험연구 |
DB 구축일자 |
2016-06-25
|
DOI |
https://doi.org/10.23000/TRKO201600003185 |
초록
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Ⅳ. 연구개발결과
1. 육량개선 사료첨가물의 개발 및 근육조직에 대한 작용기작 구명
○ 알칼로이드 계열 천연물(가지, 감자, 토마토)을 이용한 물질을 탐색완료
○ 천연물을 이용하여 건조분말, 열수추출, 에탄올 추출물 제조완료
○ 첨가물 농도별 반추위내 소화율 및 흡수농도 분석 및 도체성적 비교완료
2. 거세한우의 육량개선을 위한 사료첨가제 급여효과 구명
○ 초음파 및 biopsy이용 처리별 성장률 및 작용유전자 분석 특징구명완료
○ 감자 첨가제이용 급여량과 급여기간에 따른 성장률과 도체율 분석완
Ⅳ. 연구개발결과
1. 육량개선 사료첨가물의 개발 및 근육조직에 대한 작용기작 구명
○ 알칼로이드 계열 천연물(가지, 감자, 토마토)을 이용한 물질을 탐색완료
○ 천연물을 이용하여 건조분말, 열수추출, 에탄올 추출물 제조완료
○ 첨가물 농도별 반추위내 소화율 및 흡수농도 분석 및 도체성적 비교완료
2. 거세한우의 육량개선을 위한 사료첨가제 급여효과 구명
○ 초음파 및 biopsy이용 처리별 성장률 및 작용유전자 분석 특징구명완료
○ 감자 첨가제이용 급여량과 급여기간에 따른 성장률과 도체율 분석완료
○ 도체율 및 이화학성적분석으로 첨가물 급여량에 따른 효과분석 완료
3. 육량개선 물질이 지방과 근육조직발달에 미치는 기작 구명
○ 첨가제내 알칼로이드물질의 농도별 작용효과 분석완료
○ 첨가제 처리농도에 따른 지방산 조성변화 측정 및 분석완료
○ 처리농도별 근육 및 지방 작용기작 분석완료
Abstract
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Development of natural alkaloid-feed additive for enhancing yield grade of Hanwoo steers
The aim of this project was to determine the effect of alkaloids contained potato byproduct to muscle development of Hanwoo steers. For development of feed additive to enhancing yield grade, we searched t
Development of natural alkaloid-feed additive for enhancing yield grade of Hanwoo steers
The aim of this project was to determine the effect of alkaloids contained potato byproduct to muscle development of Hanwoo steers. For development of feed additive to enhancing yield grade, we searched three candidate plant byproducts such as potato, tomato, eggplant and screened the alkaloid compounds in the byproducts. The selected byproducts collected and dried as feed additives were used as 4 feeding trials for Hanwoo steers. The qualified alkaloids such as α-solanine and α-chaconine were treated muscle cells isolated from Hanwoo longissimus dorci and semimembranosus tissues. This study was demonstrated that the potato byproduct enhanced carcass yield grade under development of muscle tissues.
Development of feed additive for improving yield grade and study of active mechanisms to muscle tissues
Natural alkaloid such as glycoalkaloid can be alternative solution to provide growth promoters, because it had lower potential poison residue than synthetic alkaloid. Glycoalkaloid is a natural compound for synthesis of steroid hormone in the plant (Schieber et al., 2009). There is no available information in the scientific literature about application glycoalkaloid to improve development of cattle. However, potential of glycoalkaloid for enhance cattle growth is not impossible. In cultured skeletal myotubes from human and rodent, derived tomato glycoalkaloid, tomatidine, stimulated anabolism in skeletal muscle cell (Dyle et al., 2014). Other study reported, derived potato glycoalkaloid, solanidine, activate cell proliferation in vitro (Friedman et al., 2002), but high dose of glycoalkaloid becomes toxic for animal or human (Friedman et al., 1997; Nelson. 2010). Therefore, effects of glycoalkaloid need to evaluate in beef cattle nutrition depends on dose titration. This study was indicated that both potato leaves and stems contained certain amoung of alkaloids compounds which digested in the rumen of Hanwoo steers. Three candidate plant byproducts such as potato, tomato, and eggplant were collected and dried for making feed additives. Among the candidate byproducts, potato extract was contained 2.05±0.27 mg/g of α-chaconine and 0.60±0.08mg/g of α-solanine. These glycoalkaloids was potentially activated as functional compounds to skeletal muscle in beef cattle. However, tomato and eggplant byproducts were not detected any alkaloids compounds. After potato byproducts collected and dried for 24 hours, dry matter of potato byproduct were used as feeding study for evaluating active dose titrations. For evaluating dietary rate of rumen microbes, potato dry matter were treated with in situ hybridization for 72 hours using annulated Hanwoo steers. The glycoalkaloids contained potato dry matter were digested until 12 hours after treatment. Blood concentration of glycoalkaloids were not detected after 24 hours treatment of potato dry matter. These data indicated that potato byproduct were digested with rumen microbes for 24 hours after treatments. To enhancing digestibility, potato byproduct were coated with saturated palm oil for 60:40 and treated to feeding trials of Hanwoo steers.
Development of feed additive-dietary program for enhancing yield grade of Hanwoo steers
This study was indicated that dietary potato leave extract (PLE) alter growth performance and carcass characteristic of Hanwoo steers. We hypothesized that alkaloid compound in PLE, α-solanine, induced a performance, blood parameters, and muscle development in Hanwoo steers. We analyzed the degradation of α-solanine in rumen using in situ method. The α-solanine was degraded faster in rumen during 8 hours. This experiment conducted in two trials. The experimental design used randomized complete block design with trials as block factor. Observations of this experiment involved gain, ADG, blood parameters, and carcass characteristic using ultrasound and real measurement. This experiment used four pens with 4-5 steers per pen. Each pen was a treatment. Twenty (n=20) steers weighting 701.20±38.29 kg and twenty two (n=22) steers weighting 617.35±46.05 kg were allotted for Trial 1 and Trial 2 successively. Four different diets of PLE were 1) without PLE (T0) as control, 2) 100 g PLE during 60 d of feed (T1), 3) 50 g PLE during 60 d of feed (T2), 4) 100 g PLE during 40 d of feed (T3). Different PLE diet had no significant effect in gain and ADG (P>0.05). However, dietary PLE produced the higher gain and ADG than control (P<0.05). In blood parameters, dietary PLE inhibited the concentration reduction of NEFA in blood (P<0.05) and numerically decreased concentration of IP (P<0.1). Dietary PLE increased ultrasound measurement of LMA for the feed of day compared to control (P<0.05). Real measurement showed dietary PLE did not significant affect (P>0.05) in carcass characteristic. Treatment used PLE has not been significantly effect because high degradation of α-solanine in rumen. Therefore, animal did not optimally to absorb this alkaloid. Collectively, these result indicated that dietary PLE tended to increase animal production and had potentially as growth promoters.
Study of active mechanisms for developing Fat and Muscle of Hanwoo steers under dietary feed additives
This study was indicated that potato byproduct contained alkaloid compounds altered bovine satellite cell differentiation isolated by Hanwoo longissimus dorci or semimembranosus muscle tissue. We hypothesized that alkaloid compound in potato byproduct, α-solanine or α-chaconine, induced muscle development but reduced adipose development in Hanwoo steers. Intramuscular adipose tissue was collected between the 10th and 13th rib from longissimus muscle and cultured in growth media consisting of DMEM, 10% fetal bovine serum, and antibiotics at 37℃ under a humidified atmosphere of 95% O2 and 5% CO2. For the isolation of bovine satellite cells, approximately 500 g of muscle tissue was dissected from the semimembranosus muscle and transported to the cell culture laboratory. Satellite cell isolation was conducted as previously described by Johnson et al. (1998). The cells were treated with differentiation media from 0.001μM to 10μM of α-solanine and α-chaconine. Real-Time RT-PCR was used to measure not only the quantity of C/EBPβ, PPARγ, SCD, and GLUT4 mRNA for intramuscular adipocyte but also the quantity of myogenine, β-adrenergic receptor, GLUT4, MHC1, and MHC2X mRNA for bovine satellite cells relative a ribosomal protein subunit 9 mRNA in total RNA. Concentration of 10μM of α-solanine and α-chaconine have toxic effect to both bovine stellite cells. However, dose titrated levels of α-solanine and α-chaconine increased MHC 2X in both bovine satellite cells. LD muscle cells have more sensitive effect with alkaloids compound than SM muscle cells. The level of solanine and chaconine treatment not affect adipogenic gene expressions. Only SCD levels decreased at subcutaneous adipocytes. These results demonstrated that the alkaloids compound in potato byproduct increased myogenic mRNA such as MHC2X but not much affect to intramuscular adipose tissue.
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