초록

Ⅳ. 연구개발결과
메타지노믹스를 이용하여 국내 복숭아(11종), 자두(5종), 살구(3종), 매실(3종) 등 바이러스 13종 및 바이로이드 2종 등 총 15종 알려진 핵과일 바이러스 및 바이로이드 동정하였으며, 동정된 바이러스 유전체 연구 및 바이로이드 변이 연구를 수행하였다. 또한 동정된 주요 바이러스 6종 및 2종 바이로이드 진단용 RT-PCR 프라이머 제작하였다.

Abstract ▼

Stone fruit frees such as peach, apricot, plum, and Japanese plum, which are members in the Prunus species were frequently infected by different viruses and viroids via grafting and clonal propagation. Therefore, it is necessary for stone fruit trees to generate virusfree seedlings. For that, the in

Stone fruit frees such as peach, apricot, plum, and Japanese plum, which are members in the Prunus species were frequently infected by different viruses and viroids via grafting and clonal propagation. Therefore, it is necessary for stone fruit trees to generate virusfree seedlings. For that, the information about viruses and viroids infecting Prunus species is required. In this study, we carried out metagenomics to establish viruses and viroids infecting Prunus species. We sampled virus-infected leaves from various Prunus species and generated libraries for next generation sequencing. After paired-end sequencing using HiSeq2000 system, we de novo assembled transcriptomes of various Prunus species using Trinity program. After blast search against known plant viral database, we identified a total of 13 known viruses and two viroids infecting various stone fruit trees. In addition, we analyzed quasispecies of identified viruses and viroids. Based on identified viral genomes, we designed RT-PCR primer-pairs to detect six viruses and two viroids. Taken together, our study provides a comprehensive overview of viruses and viroids infecting Prunus species in Korea and generated RT-PCR primer-pairs which can be usefully applied in virus detection as well as to produce virus-free stone fruit trees. The approaches in our study can be successfully applied to detect known and novel viruses infecting other economically important crops.