초록

Ⅳ. 연구개발결과
- 파골세포에서 한속단추출물의 TRAP활성 및 세포생존율 측정한 결과 농도의존적으로 TRAP활성을 저해
- 한속단의 국내외 유전자원을 수집 및 특성 조사
- 전사체 분석 완료 및 terpenoid 생합성에 관련된 유용 유전자 분리, 관련 유전자의 전사체발현량을 분석, 부위별 주요 성분 분석 완료
- 한속단 원료 표준 제조 공정을 확립
- 한속단 및 유사생약에 대한 확인시험법을 개발
- 한속단의 특성을 입증할 수 있는 지표성분 및 프로파일 분석법을 개발

Abstract ▼

Phlomis umbrosa (P. umbrosa) in herbal medicine has been used as a robustness, analgesic, anti-inflammatory, and plant origin is different from country to country. The root of Phlomis umbrosa Turcz.(Labiatae) is used in Korea, the root of Dipsacus asper Wall.(Dipsacaceae) in China, the root of Cirsi

Phlomis umbrosa (P. umbrosa) in herbal medicine has been used as a robustness, analgesic, anti-inflammatory, and plant origin is different from country to country. The root of Phlomis umbrosa Turcz.(Labiatae) is used in Korea, the root of Dipsacus asper Wall.(Dipsacaceae) in China, the root of Cirsium sp.(Compositae) in Japan. D.asper and P.umbrosa that differ in plant taxonomy has often been confused in Korea. Most of the studies for P.umbrosa has been done by collection. Therefore, it is necessary that the seed and root of P.umbrosa is developed standardization and optimization of cultivation technique.
First, we evaluated P. umbrosa extract and its derivatives effects on cell growth. RAW264.7 cells were treated with P. umbrosa extract and its compounds at different concentrations (3 μg/ml, 10 μg/ml, 30 μg/ml and 100 μg/ml) and the cell growth was measured by MTT assay. Next, we evaluated the inhibitory activity of P. umbrosa extract and its derivatives RANKL-induced osteoclastogenesis in RAW 264.7 cells. We found TRAP activity was decreased by P. umbrosa extract and acteoside, as determined by measuring the cellular TRAP activity. Their anti-osteoclastogenic effects were not attributed to cell toxicity.
Second, we aimed at development of mass production method of P. umbrosa. In order to achieve this purpose, we collected genetic resources of P. umbrosa from different locations in Korea and other countries. We collected seeds from 1,466 plants and propagative roots from 25 plants in various locations including Jecheon, Chungbuk.
Third, this study was carried out to have the major components of the subterranean part of Korean Phlomis umbrosa (P. umbrosa), Dipsacus asperoides (D. asperoides) as and of the one collected from the two regions of China. Through a transcriptome analysis, identify genes involved in the biosynthesis of major component, based on which a comparative analysis was done on gene division, component content in each part of the plant body, etc. This study has been conducted to obtain basic data for cultivation techniques with improving productivity and quality of P. umbrosa.
Next, Standard manufacturing process for P. umbrosa was established by conducting physicochemical analysis of the extract. In addition, identification method for P.umbrosa and other closely related species were developed, and marker compounds and profile analysis method that can demonstrate characteristics of P.umbrosa were also developed. Gathering diverse genetic resources of P.umbrosa to select and cultivate representative sample can contribute increased sales in domestic farm and extended range of food, health functional food, and drug development. Additionally, accumulated physicochemical profiling data of P.umbrosa cultivated in Korea possesses great value as technical data for international commercialization.