Abstract ▼

The research was conducted in 5 categories.
1. Development of doping test method for officials imposed physical fitness test
The analytical method for 39 drougs prohibited drugs - 7 analbolics, 3 diuretics,3 stimulants and 11 narcotics and 15 their metabolites- was developed and successfully a

The research was conducted in 5 categories.
1. Development of doping test method for officials imposed physical fitness test
The analytical method for 39 drougs prohibited drugs - 7 analbolics, 3 diuretics,3 stimulants and 11 narcotics and 15 their metabolites- was developed and successfully applied about 750 samples.
2. Development and validation of urine samples related with treatment and custody
The aim of the study was to develop and validate a simple, sensitive and specific method for the detection of 65 substances among psychoactive drugs and their metabolites in whole blood, and to apply the procedure to parolee cases. The method proved to be highly sensitive and specific. And all the validation parameters fulfilled the acceptable criteria.
3. Deveopment of nano-analytical method using Dried Blood Spot (DBS) to analyze NSAIDs
Dried Blood Spot (DBS) is a new sample preparation method that can be used for qualification and quantification with much smaller blood volume than solid-phase extraction (SPE) & liquid-liquid extraction (LLE). DBS and it can be applied in forensic toxicology and illicit drug analysis field. In this study, 19 NSAIDs(non-steroidal anti-inflammatory drugs) were extracted in blood sample with DBS and analyzed with LC-MSMS. After testing for for the kind of DBS card, extraction solvent and time, and the conditions of LC-MS/MS, the most effective method were developed and validated.
4. Development of analytical method to analyze antibiotics
Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death. Drugs, especially an antibiotic from the penicillin or cephalosporin group, a "sulfa" antibiotic are included. We develop method for the determination simultaneous 34 kinds of antibiotics in whole blood. by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) developed for the simultaneous determination of 34 antibiotics. Linearity in the calibration was obtained over a range of 0.1-100 ng/mL of antibiotics. It showed that limitation of detection and quantification of most drugs is 0.5-1 ug/L. Absolute recoveries and relative recoveries are over 75% for most antibiotics. The determination of related drug with anaphylaxis in postmortem blood should be useful indicator for identification of the cause of death.
5. Developemth of standard solution certification method using NMR ERETIC method
NMR ERETIC method was adopted in quantitative analysis of 30 drugs standard solutions. Stock solutions evaluated by the method will be supplied in regional institutes.