초록

□ 치조골내 발현 단백질 분석방법 개발
1. 골조직내 차등발현 발굴을 위한 골단백질의 순차적 추출방법 확립 및 적용
2. 치조골재생 및 골흡수제어용 골단백질 10종이상 동정
3. 합성골 처리에 의한 치조골재생용 단백질의 발현변화 검증
□ 단백질간 상호작용 분석을 통한 치조골 재생기전 규명
1. 발현변화 골단백질의 네트워크 분석을 통한 작용점 규명
2. 합성골 처리에 의한 세포내 환경변화 분석 및 작용점 예측
3. 발굴인자의 분자생물학적 특성 및 재생 가능성 평가

Abstract ▼

I. The purpose
- This study about alveolar bone regeneration would lead to prevent tooth and alveolar bone loss caused by periodontal disease and further more would contribute to improve the quality of public health care by delaying the implementation of the problems caused by alveolar bone loss.

I. The purpose
- This study about alveolar bone regeneration would lead to prevent tooth and alveolar bone loss caused by periodontal disease and further more would contribute to improve the quality of public health care by delaying the implementation of the problems caused by alveolar bone loss.
- There are needs for fundamental treatment to overcome the limitations of medical treatment for the diverse choices and patient-specific therapy using biological materials.
- This study seeks to find the novel target proteins for bone regeneration by the hard tissue proteomics analysis and to identify new regulation mechanism in bone regeneration and metabolism regulation.

II. The contents
[Research content 1]
▶ Establishment of protein extraction method from hard tissue using animal models (micro-pig)
[Research content 2]
▶ Establishment of analytical method for shotgun proteomics
▶ Investigation of the proteins associated with bone regeneration and resorption: Identification of biochemical
characterization
[Research content 3]
▶ Investigation of the point of application of the identified protein
- Validation of effect on bone regeneration and osteoblast differentiation
- Identification of cell signaling pathway with a focus on discovering protein

III. The result
▶ Development of analytical method for protein expression within alveolar bone tissue
1. Establishment and application of sequential extraction method to identify the proteins displaying differential expression within the bone tissue
2. Identification of more than 10 target proteins which associated with regulation of bone regeneration and resorption
3. Validation of expression changes of proteins in alveolar bone regeneration by treatment of synthetic bone
▶ Identification of bone regeneration mechanism by analyzing the protein-protein interaction
1. Identification of the point of application by analyzing network of bone-derived proteins
2. Prediction of cellular environment and point of application due to treatment of synthetic bone
3. Evaluation of molecular biological characteristics and reproducibility of the excavation factor

IV. Application plan
▶ Providing a new paradigm for the mechanism of bone regeneration research by analyzing the bone-derived protein interaction
▶ Applying the analytical target to the development of biomaterials and offering key sources of clinical applications
▶ Using as a research model for the various biological materials for bone regeneration