초록 ▼

생체연결핀 펩타이드 설계 및 제조와 세포내 분포 평가 및 세포간 결합 1차 효능 평가를 통해 두가지 형태의 생체 연결핀 제조 및 세포 모델 효능 평가 완료하였음. 이들의 생물학적 기능평가 및 생체연결핀 기능 개선으로 효능평가와 여러 ex vivo 모델에서 생체 연결핀 세포-조직 결합 평가 완료 하였음. 동시에 생체연결핀의 생물학적 기능 평가를 통해 tight junction protein의 발현 촉진 현상을 도출하였고, in vivo mouse 모델 (stereotaxic ventricle model)을 이용한 세포전달 효능, ra

생체연결핀 펩타이드 설계 및 제조와 세포내 분포 평가 및 세포간 결합 1차 효능 평가를 통해 두가지 형태의 생체 연결핀 제조 및 세포 모델 효능 평가 완료하였음. 이들의 생물학적 기능평가 및 생체연결핀 기능 개선으로 효능평가와 여러 ex vivo 모델에서 생체 연결핀 세포-조직 결합 평가 완료 하였음. 동시에 생체연결핀의 생물학적 기능 평가를 통해 tight junction protein의 발현 촉진 현상을 도출하였고, in vivo mouse 모델 (stereotaxic ventricle model)을 이용한 세포전달 효능, rat 심근경색 모델에서 줄기세포 전달 효능을 검증하였음

Abstract ▼

I. Purpose of project
To design and prepare penetrating- or attaching-cell connectors to improve transduction and function of stem cell therapy. And to select and evaluate the cell connector using cell-cell interaction test and ex vivo/in vivo models.

Ⅱ. Contents
- Design and preparatio

I. Purpose of project
To design and prepare penetrating- or attaching-cell connectors to improve transduction and function of stem cell therapy. And to select and evaluate the cell connector using cell-cell interaction test and ex vivo/in vivo models.

Ⅱ. Contents
- Design and preparation of penetrating- or attaching-cell connectors
- Evaluation of efficacy for cellular distribution and cell-cell interaction
- Evaluation of biological function and improvement of its function
- assessment of cell connectors using ex vivo models
- Efficacy test using in vivo mouse stereotaxic ventricle model
- Efficacy test using rat myocardial infarction model

Ⅲ. Results
- Desing and preparation of cell connector: Completion of design and preparation of two types of cell connectors by recombinant technology and peptide synthesis, and deduction of their efficacy using cellular distribution and cell-cell interaction.
- Evaluation of biological function and improvement of its function: Improvement of cell connector by in vitro cell-cell interaction models and ex vivo mouse tail and beef aitchbone models.
- In vivo efficacy test: Deduction of efficacy of cell connector using in vivo stereotaxic ventricle models, ex vivo Langendorff system and rat myocardial infarction model. And cell connector induces cellular tight junction proteins.

Ⅳ. Application plan
- Application of stem cells-related biomaterials, bio-tissue/organ system, cell therapies, and research tool in the filed of biotechnology and biomedicine.
- Deduction of follow-up research projects for stem cell application technology, cell/tissue transplantations, functional peptide materials, and practical use and clinical applications.