초록 ▼

Ⅳ. 연구개발결과
일년생 바이오매스작물 중 수수/수단그래스 교잡종의 건물수량이 가장 많고 BMR이 발현되는 Green Star와 대마 자웅동주 36개체를 선발하였다. 수수/수단그래스 교잡종의 Green star가 가장 발효율이 92.4%로 가장 우수하였으며 에탄올 생산량은 6200L/ha로 예측되었다. 쌀보리에 비해 겉보리의 보릿짚 수량 수량이 높았고 Cellulose 및 Hemicellulose 등 발효기질 수량이 1,000㎏/10a 이상으로 유망한 것은 광안보리 등 2 품종이었다. 고구마 최적 당화조건에 의해 생성된 환원당

Ⅳ. 연구개발결과
일년생 바이오매스작물 중 수수/수단그래스 교잡종의 건물수량이 가장 많고 BMR이 발현되는 Green Star와 대마 자웅동주 36개체를 선발하였다. 수수/수단그래스 교잡종의 Green star가 가장 발효율이 92.4%로 가장 우수하였으며 에탄올 생산량은 6200L/ha로 예측되었다. 쌀보리에 비해 겉보리의 보릿짚 수량 수량이 높았고 Cellulose 및 Hemicellulose 등 발효기질 수량이 1,000㎏/10a 이상으로 유망한 것은 광안보리 등 2 품종이었다. 고구마 최적 당화조건에 의해 생성된 환원당을 이용한 에탄올 발효 결과 24시간동안 65.8g-EtOH/L의 에탄올을 생산하였다. 식품원료용 대마 종실 및 기름의 THC 함량 허용기준을 10ppm으로 설정하였다.

Abstract ▼

Recently, abandoned land were increase because of control police for rice production by government. Growing alternative crop for rice including soybean is difficult without deforming the rice paddy because the land will be used for growing rice for emergencycase including food shortage. Annual biom

Recently, abandoned land were increase because of control police for rice production by government. Growing alternative crop for rice including soybean is difficult without deforming the rice paddy because the land will be used for growing rice for emergencycase including food shortage. Annual biomass crops are good choice for using the land because the crops can be grown without deforming the rice paddy. Hemp is anther good choice for using the abandoned land because it can be used for production bioenergy using the stem and food using the seed. But it is difficult to use the seed for food because seed yield is low and hemp seed food is illegal in Korea.

This study was carried out to select annual biomass crop for production of second generation bioethanol by evaluating yield and growing characteristics of amaranth(Amaranthus palmeri L.), pearlmillet(Pennisetum americanum L.), sorthum-sudangrass hybrid, corn(Zea maize L.), kenaf(Hibiscus cannabinus L.) and hemp(Cannabis sativa L.). And in order to increase hemp seed yield and to be used hemp seed for food material, selected dioecious lines of hemp and reviewed legislation and literature relation to hemp seed food.

Among the evaluated crops, sorthum-sudangrass hybrid was best annual biomass crop because of its good productivity of biomass. Green Star, the cultivar of sorthum-sudangrass hybrid, was selected for production of second generation bioethanol because of its biomass productivity of 46.9t/㏊ and strong expression of BMR(Brown Mid-Rib). We selected 36 lines of dioecious hemp to increase hemp seed yield. We recommended acceptable THC level of hemp seed to 10 ppm for food material.

In this study, the agronomic characteristics of covered and naked barley cultivars grown in Korea were studied. Neutral detergent fiber(NDF), Acid Detergent fiber(ADF), Acid Detergent Lignin (ADL) were analyzed as stem characteristics, and stem yield was investigated to evaluate the total cellulose yield. Total stem yield of covered barley was 395～591 kg/10a, Hemi had the highest yield. In the case of naked barley, total stem yield was 261～587 kg/10a, Nahanssal had the highest yield. Covered barley cultivars were higher than naked barley cultivars. Hight of barley cultivars was 71-94 cm in covered barley and 57-88 cm in naked barley. Hight was positively correlated with stem yield. Hemicellulose and cellulose content of covered barley was 21.1-26.8 % and 33.6-40.5 %, respectively. Keunalbory 1 had the highest hemicellulose content and Kwangan had the highest cellulose contemt. Lignin contemt was 4.7-8.6 % and Hemi had the lowest content.

Cellulose yield was 246.0-376.4 kg/10a and Saegang and Kwangan showed the high cellulose yield. Hemicellulose and cellulose content of naked barley was 18.6-28.2 % and 30.2-39.3 %, respectively. Hobanchal had the highest hemicellulose content and Donghanchal had the highest cellulose contemt. Lignin contemt was 4.4-11.2 % and Hinchal had the lowest content. Cellulose yield was 141.6-358.6 kg/10a and Nahanchal, Chungho and Donghanchal showed the high cellulose yield. Composition analysis was performed for selection of variety to efficiently produce bioethanol on 14 varieties of hybrid sorghum sudan grass. The content of cellulose, hemicellulose, lignin and ash of these varieties was 32~39%, 19~24%, 17~22% and 6~11%, respectively. Among these varieties, 4 varieties of hybrid sorghum sudan grass (Green Satr, Revolution, KF429, SS504) were selected for the evaluation of ethanol yield and those were pretreated with 1M NaOH solutionat at 150℃ for 30minutes using high temperature explosion system. Pretreated samples contained 52~57% of cellulose. The ethanol productions yield was evaluated by simultaneous saccharification and fermentation (SSF) for the development of bioethanol production techniques using sodium hydroxide pretreated hybrid sorghum sudan grass. SSF fermentation was carried out for 48hours at 33℃ by Saccharomyces cerevisiae CHY1011 using Green star variety which celluose content was the highest. The ethanol yield was 92.4% and ethanol productions yield was estimated 6200 L/ha.

To optimize the saccharification of sweetpotato for bio-ethanol production, the effect of liquefaction conditions was investigated by response surface methodology (RSM). A 2³ factorial central composite design (CCD) was chosen to explain the combined effects of the liquefaction conditions of sweetpotato, viz. biomass content, temperature of liquefaction, and liquefaction time. Concentration of reducing sugar and saccharification yield were the response (dependent) variables. The model adequacies were very satisfactory, as coefficients of determination (R²) of response surface regression equation for concentration of reducing sugar and saccharification yield were 0.9737 and 0.9549, respectively. Optimal conditions for saccharification were determined as follows: biomass content, 18.35%; temperature of liquefaction, 94℃; liquefaction time, 59.7 min. The model predicted that maximum reducing sugar concentration which can be obtained using the above optima conditions of variables was 146.8 g/L. Similar ly, experimental result of saccharification was obtained as 152.0 g-reducing sugar/L and this result was in close agreement with the model prediction. 65.8 g-EtOH/L was produced from obtained reducing sugar for 24h and ethanol yield was 84.8%. The addition of xylanase improved the sweetpotato loading up to 30% and bioethanol production was increased by high sweetpotato loading.

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 요 약 문 ... 3
• SUMMARY ... 4
• 제1장 서 론 ... 6
• 제1절 일년생 바이오매스작물 선발 ... 6
• 제2절 일년생 바이오매스의 전처리 조건 확립 ... 7
• 제3절 전분질계 바이오에탄올 생산기술 확립 ... 8
• 제4절 바이오에탄올 생산용 맥류 중간 모본 개발 및 최적 맥류 품종 개발 ... 8
• 제2장 국내외 기술개발 현황 ... 10
• 제1절 일년생 바이오매스작물 선발 ... 10
• 제2절 일년생 바이오매스의 전처리 조건 확립 ... 10
• 제3절 전분질계 바이오에탄올 생산기술 확립 ... 11
• 제4절 바이오에탄올 생산용 맥류 중간 모본 개발 및 최적 맥류 품종 개발 ... 12
• 제3장 연구개발수행 내용 및 결과 ... 13
• 제1절 일년생 바이오매스작물 선발 ... 13
• 제2절 일년생 바이오매스의 전처리 조건 확립 ... 23
• 제3절 전분질계 바이오에탄올 생산기술 확립 ... 30
• 제4절 바이오에탄올 생산용 맥류 최적 품종선발 ... 41
• 제4장 연구개발목표 달성도 및 대외기여도 ... 50
• 제1절 목표대비 달성도 ... 50
• 제2절 정량적 성과 ... 51
• 제5장 연구개발결과의 활용계획 ... 53
• 제6장 중요 변동사항 ... 53
• 제7장 참고문헌 ... 54
• 끝페이지 ... 57