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Kafe 바로가기주관연구기관 | 부산대학교 산학협력단 Busan National University |
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연구책임자 | 정영진 |
참여연구자 | 이광수 , 손홍주 , 황대연 |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 | 한국어 |
발행년월 | 2015-09 |
과제시작연도 | 2012 |
주관부처 | 해양수산부 Ministry of Oceans and Fisheries |
등록번호 | TRKO201600011050 |
과제고유번호 | 1545003997 |
사업명 | 수산실용화기술개발사업 |
DB 구축일자 | 2021-10-09 |
키워드 | 미더덕껍질.창상관리제품.메디컬 파이버.마스크 팩.산업화.Tunic Styela clava.wound medicine.medical fiber.mask pack.Industrial application. |
DOI | https://doi.org/10.23000/TRKO201600011050 |
○ 폐자원인 미더덕 껍질을 이용한 고부가가치 소재 개발 및 산업화를 위하여 친환경 용매인 이온성 액체를 이용한 셀룰로오스 용해법을 확립하고 이를 이용하여 창상치료용으로 적용가능한 필름형태의 막을 개발하였으며, 생분해성이 있으며 독성은 나타나지 않음.
○ 제조된 필름, 파이버와 동물성 봉합사 개발을 위하여 제품의 물성 및 특성 연구를 수행
○ 창상치료용 Dressing제 및 동물용 수술용 봉합사 생산을 위하여 대량 생산을 위한 장비개발 및 양산체계 구축 위한 연구 수행
○ 미더덕껍질로부터 추출물을 분리한 후 생리활성을
○ 폐자원인 미더덕 껍질을 이용한 고부가가치 소재 개발 및 산업화를 위하여 친환경 용매인 이온성 액체를 이용한 셀룰로오스 용해법을 확립하고 이를 이용하여 창상치료용으로 적용가능한 필름형태의 막을 개발하였으며, 생분해성이 있으며 독성은 나타나지 않음.
○ 제조된 필름, 파이버와 동물성 봉합사 개발을 위하여 제품의 물성 및 특성 연구를 수행
○ 창상치료용 Dressing제 및 동물용 수술용 봉합사 생산을 위하여 대량 생산을 위한 장비개발 및 양산체계 구축 위한 연구 수행
○ 미더덕껍질로부터 추출물을 분리한 후 생리활성을 분석한 결과 에탄올 추출물이 우수한 효과가 있음을 확인함
○ 미더덕껍질유래 생리활성물질을 함유하는 MC 개발 위해 MC 생산최적조건 및 생성물의 역학적 특성을 조사함
○ 미더덕껍질유래 생리활성성분과 MC를 융합함으로써 신개념 마스크 팩 또는 spot band 개발 가능성을 조사
○ 추출물 및 필름에 대한 독성과 항산화능에 대한 평가를 실시하여 세포가 잘 부착하여 성장하였고, 특이적인 독성은 관찰되지 않음
○ 미더덕껍질 필름의 생분해성 평가하는 동물실험에서 점진적으로 분해가 진행되었으며 특이한 독성은 없었음
○ 개발된 제품에 대한 생분해성, 독성, 창상치료효능, 항노화효과 등을 모델동물을 이용하여 분석하여 효능을 검증
○ 생산된 제품에 대한 독성평가, 효능평가 및 허가자료확보를 통해 기업의 허가절차 추진을 지원함
○ 실상처치료용 필름제조기법을 공장수준에 적용될 수 있도록 미더덕껍질의 전처리, 분쇄방법, 이온성 액체를 이용한 셀룰로오스 용해와 필름제조 기법을 확립
○ 미더덕껍질 추출물을 이용하여 마스크팩 시제품을 개발하여 소비자 테스트를 수행하였고, 제품의 디자인, 마케팅 전략 등을 수립함
○ 상처치료용 필름 및 파이버의 생산체계를 구축하기 위한 생산 및 제품의 특성을 연구함.
○ 개발된 미더덕껍질 제품의 인허가에 필요한 자료를 확보, 제품의 배합 및 공정조건, 마케팅, 홍보전략 수립. 대량생산 및 제품의 판매를 위한 체계를 확립
Ⅳ. Results of research and development
For the development and industrialization of Styela clava tunic disposed as wastes, the pre-treatment method removing impurities in materials was established. In order to develop film and fiber using cellulose from Styela clava tunic, it was pulverized a
Ⅳ. Results of research and development
For the development and industrialization of Styela clava tunic disposed as wastes, the pre-treatment method removing impurities in materials was established. In order to develop film and fiber using cellulose from Styela clava tunic, it was pulverized and sucessfully dissolved in eco-friendly ionic liquid, thereby producing cellulose film for wound care treaties.
It was performed for studies on the natural and enzymatic biodegradations in order to evaluate the performance as a biological material of the prepared films and fibers from Styela clava tunic, as well as the toxicity using a skin cell line, showing that the prepared films and fibers from Styela clava tunic is similar or better biodegradable than the common pulp and it did not show cytotoxicity. Absorption, moisture permeability, and mechanical strength of the films was performed to analyze their physical properties. In case of fibers, properties of spinning thread was analyzed. In addtion, the basic experiment was conducted for the animal surgical fiber through the comparison with commercial wound care products.
We tried to develop wound care products to secure the domestic and overseas markets, because wet type of wound care products(hydrogel, hydrocolloid dressings, alginate, etc.) is rapidly increasing its consumption in the developed countries and it shows good healing and scar prevention effect. We carried out to develop equipment for mass production for the wound care dressings, and animal surgical fiber threads, organized the team for the continuous production, and develop the standard methods.
To prepare extracts excellent in biological activities and develop mask pack using them from Styela clava tunic, various extracts were prepared using different kinds of extraction solvents and their propeties were analyzed. Among them, ethanol extract showed higher whitening activity, moisturizing activity, anti-aging activity, anti-wrinkle activity, antioxidant activity. Based on this, the extract was provided to collaborative research teams for them to evaluate efficacy test and to develop trial manufactured goods.
The optimal production conditions by selected strains(broth composition, culture conditions and the culture medium surface area, etc.) were set up to develop microbial cellulose(MC) containing biologically active compounds from Styela clava tunic, and the microstructure of MC, water holding capacity, tensile strength and elongation were evaluated for mechanical properties. As a result, we found the fermentation conditions for the higher production than the commonly used medium.
The productivity of MC was evaluated according to type and concentrion of the extract, showing potentials for the mass production of MC. In addition, the possibility of MC production was confirmed by impregnating the MC with extracts from Styela clava tunic. In other words, we introduced a new concept of development potential for mask pack or spot band by combining bioactive compounds and microbial cellulose.
In order to perform the efficacy of the extract and the film prepared by collaborative research teams, culture conditions and cytotoxicity were evaluated, and antioxidative activity was tested. Antioxidative activity showed a little discrepancy compared to in vitro test, but cytotoxicities of samples were not detected. Cells were attached and grew well on film which did not show any toxicity toward cells. The film was degraded gradually during the 3 month experiment but did not show any toxic effect on animals.
Biodegradability, toxicity, wound healing efficacy, and anti-oxidative activity of the products developed by collaborative research teams were conducted using animal model. For this purpose the securing technique for establishing an animal scald model and animal wound model was reliably established. Film was implanted into the subcutaneous tissue of rats, and it was gradually degraded and it did not induce specific toxicity and a significant change in the immune indices. Hydrocolloid wound care products prepared by Styela clava tunic induced regeneration of artificially developed skin wounds, inhibited the infiltration of mast cells to induce inflammatory reactions effectively, and showed the effect of promoting the synthesis and deposition of collagen. These extracts showed the inhibition effect of anti-skin aging induced by UV irradiation.
Toxicity, efficacy and documents for KFDA permission of the products prepared by collaborative research teams were performed. At first, for the test, test protocols was established according to the guidance of the authorized institution. Base on this, toxicity test, physiological activity tests, efficacy test, and biological activity were analyzed. We are tried to prepare the written data required for the permission of the products by the authority by organizing and analyzing their results, thus supporting for the industry to proceed the licensing process of the company.
We established the standard method of prepartion of Styela clava tunic powder by pre-treatment, and pulverization using pin mill to scale up from laboratory scale to industrial scale. In addition, it established the cellulose dissolution and the film manufacturing method using an ionic liquid. Two kinds of trial manufactured mask pack were produced and evaluated on a consumer’s test. Based on these, We established the design, and marketing strategy of the product.
We studied the characteristics of the product and various melting method to establish a production system of the film and fibers for wound care. Production feasibility of Styela clava tunic for the production of MC was evaluated to establish a production system of the MC. Furthermore, consumer’s evaulation on trial manufactured product such as mask pack, and spot band containing Styela clava tunic extract was carried out.
In addition, we tried to secure the materials necessary for the licensing of products developed from Styela clava tunic, and to ensure that licensing procedures. Also, we tried to develop a formulation and process conditions, the establishment of a marketing and promotional strategy, and the economic analysis of a variety of products by establishing a system for the mass production and sale of the product.
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