초록

연구결과
□ 필요성 및 목표
병역대상자중 병역면탈을 위하여 정신질환을 위장하는 자들의 모발 중 항정신병 약물을 검출하여 진위여부를 과학적으로 증명하고자 함.

□ 방법 및 결과
모발 중 항정신병 약물 15종 및 대사체 2종에 대한 동시 분석법을 개발하고 유효화를 시행하였음.

□ 기대성과
다양한 분야의 감정기법을 개발함으로써 모발 감정영역 확대 및 해당분야에서 국과수의 선도적 기술력 확보

( 출처 : 연차실적·계획서 -요약 )

Abstract ▼

In recent years, the inappropriate use of antipsychotics by young Korean men has become a social problem. As military service exemptions are given for mental illness, some men pose as mental health patients to avoid military service. In order to verify the authenticity of mental illnesses, we develo

In recent years, the inappropriate use of antipsychotics by young Korean men has become a social problem. As military service exemptions are given for mental illness, some men pose as mental health patients to avoid military service. In order to verify the authenticity of mental illnesses, we developed simultaneous analytical methods for the detection of 15 antipsychotics and 2 of their metabolites in hair using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The target drugs were modafinil, atomoxetine, aripiprazole, benztropine, buspirone, duloxetine, gabapentin, oxcarbazepine, topiramate, escitalopram, paliperidone, ziprasidone, lamotrigine, clonazepam, levetiracetam, and metabolites of oxcarbazepine and clonazepam. To remove possible contaminants on the hair surface, hair samples were washed twice with methanol and distilled water, and then were extracted with methanol overnight at 38 ℃. Desipramine-d3 was used as the internal standard. LC-MS/MS analysis was performed on an Agilent 1290 Infinity UHPLC coupled to an AB Sciex Qtrap® 5500 MS/MS. The total chromatographic run time was 14 min. The following validation parameters were evaluated: selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, matrix effect, and recovery. The LOD and LOQ values for all analytes, except modafinil, ranged from 0.002 to 0.1 ng/10 mg and from 0.002 to 0.2 ng/10 mg, respectively. Good linearity was achieved for most of the analytes in the range of 0.5 - 2 ng/10 mg. The method showed acceptable precision and accuracy, which were less than 15%, as well as satisfactory matrix effects and recoveries. Furthermore, this method was also applied to the analysis of rat hair samples. The study in rats showed that the concentrations of atomoxetine and aripiprazole in pigmented hair were significantly higher than those in non-pigmented hair. However, no significant difference was observed in the concentration of topiramate between pigmented and non-pigmented hair. This method will be useful in monitoring the inappropriate use of antipsychotics in suspects posing as mental health patients. However, further research is necessary before applying this method to authentic hair samples from mental health patients.

( 출처 : SUMMARY )

목차 Contents

• 표지 ... 1자체연구과제 연차실적·계획서 ... 2요 약 문 ... 4Summary ... 5목차 ... 6제1장 연구과제의 개요 ... 7 제1절 연구과제의 목표 ... 7 제2절 연구과제의 필요성 ... 7제2장 총괄연구과제의 국내·외 연구 현황 ... 8제3장 연구과제의 연구수행 내용 및 결과 ... 9제4장 연구과제의 연구결과 고찰 및 결론 ... 20제5장 연구과제의 목표달성도 및 관련분야에의 기여도 ... 24 제1절 목표달성도 ... 24 제2절 관련분야 기여도 ... 24제6장 연구과제 연구 결과 활용계획 ... 25 제1절 활용성과 ... 25 제2절 활용계획 ... 28제7장 첨부서류 ... 29끝페이지 ... 29