초록 ▼

30 MeV 사이클로트론을 활용해 모핵종인 68Ge를 생산하고 이를 지지체에 흡착시켜 일정시간 후에 딸핵종인 68Ga를 용출하여 PET용 방사성의약품의 개발에 활용할 수있는 68Ge/68Ga 발생장치의 개발과 용출된 68Ga 동위원소를 활용하는 기술 개발을 목표로 다음의 연구를 수행하였다.
1. 68Ge 생산 표적개발 및 68Ge 생산
- 30MeV 사이클로트론에서 68Ge을 생산하기위한 밀폐형 구조의 표적 개발
- 표적재질 적합성 평가연구 및 양성자 빔 조사
2. 68Ge 생산/분리 및 발생장치용 흡착재 개발

30 MeV 사이클로트론을 활용해 모핵종인 68Ge를 생산하고 이를 지지체에 흡착시켜 일정시간 후에 딸핵종인 68Ga를 용출하여 PET용 방사성의약품의 개발에 활용할 수있는 68Ge/68Ga 발생장치의 개발과 용출된 68Ga 동위원소를 활용하는 기술 개발을 목표로 다음의 연구를 수행하였다.
1. 68Ge 생산 표적개발 및 68Ge 생산
- 30MeV 사이클로트론에서 68Ge을 생산하기위한 밀폐형 구조의 표적 개발
- 표적재질 적합성 평가연구 및 양성자 빔 조사
2. 68Ge 생산/분리 및 발생장치용 흡착재 개발
- 안정동위원소를 이용합 분리법 개발 및 68Ge 생산표적으로부터 68Ge 분리
- 금속산화물과 금속인산염을 이용한 68Ge/68Ga 분리용 흡착제 개발
3. 68Ga 활용 방사성의약품 개발
- 생물학적 연구를 통한 흑색종 피부암 진단용 68Ga 표지 방사성의약품 개발
- 나노기술을 이용한 68Ga 표지 나노 덴드리머 혼성체 개발
4. 발생장치용 차폐체 및 케이스 설계/제작
(출처: 보고서 요약서 3p)

Abstract ▼

Ⅳ. Result
1. Target development for 68Ge production and production of 68Ge the property evaluation of various metals was performed for the application to Ga encapsulation. Ti, Nb, and Mo foils were heated in the liquid Ga environment for 2 weeks. According to the result of scanning electron micro

Ⅳ. Result
1. Target development for 68Ge production and production of 68Ge the property evaluation of various metals was performed for the application to Ga encapsulation. Ti, Nb, and Mo foils were heated in the liquid Ga environment for 2 weeks. According to the result of scanning electron microscopy, a Nb foil showed the least amount of Ga adhesion while a Ti foil exhibited the most amount of Ga adhesion. Results of energy dispersive X-ray spectroscopy and X-ray diffraction analysis displayed that Ga just physically adhered to metal foils without any chemical bonding. Finally, result of X-ray photoelectron spectroscopy revealed that Nb was oxidized by heating in the liquid Ga while Mo was rather partially reduced. Comprehensively, Mo can be used as an encapsulatation material of Ga compared to Nb which is a conventional encapsulation material of Ga. natGa(p,xn)68Ge was selected as a nuclear reaction for the production of 68Ge using natural Ga target. Based on the result of SRIM code simulation, proton energy of 30 MeV and target irradiation angle of 45o were determined, and then, 68Ge target was design and manufactured. Use of this target, we produced 68Ge with cyclotron at KIRAMS.

2. Separation of 68Ge and development of column material and extraction system for 68Ge/68Ga separation
Germanium and gallium within mixed solution were respectively separated by using a liquid-liquid extraction and a column chromatographic method. The separation of Ge within high concentrated hydrochloric and sulfuric acid was conducted by the extraction to CCl4 and the back-extraction to 0.05 M HCl. An optimum condition of the extraction by CCl4 was in 5~7M HCl and efficiency was around 80%. In case of absorbents, the metal oxides such TiO2, SnO2 and Al2O3 and the metal phosphates such as Ti phosphates, Zr phosphates and TiZr phosphates. The gallium was selectively separated by using Al2O3 among metal oxides and TiZr phsphate among metal phosphates from the mixed solution in 0.01~0.20 M HCl condition.

3. Development of 68Ga labelled radiopharmaceutical
68Ga-SCN-DOTA-PCA (68Ga labelled benzamide derivative) was synthesized with a radiochemical yield of ~80% and a radiochemical purity of >97% by analytical HPLC. The in vitro binding of 68Ga-SCN-DOTA-PCA to melanin and its cellular uptake demonstrated the selective uptake in melanin. In addition, the biodistribution and micro-PET imaging of 68Ga-SCN-DOTA-PCA in B16F10 tumor models showed the specific accumulation in melanoma. These results suggest that 68Ga-SCN-DOTA-PCA would be a promising agent for melanoma diagnosis.
[68Ga]Ga-NOTA-PAMAM dendrimer complex was rapidly labeled with NOTA-PAMAM dendrimer complex and [68Ga]GaCl3 in 15~25% yield. In order to conjugate Fe3O4 nanoparticles on NOTA-PAMAM dendrimer, Silicate encapsulated Fe3O4 (6nm) was synthesized and then surface modified Fe3O4@SiO2 core shell was prepared with PEG and dendrimer. A mixture of NOTA dendrimer conjugated Fe3O4@SiO2 core shell with [68Ga]GaCl3 in a 0.1 M sodium carbonate buffer (pH 9.5) was stirred for 20 h at room temperature in the dark. Based on these results, we are currently developing 68Ga-labeled nano-dendrimer composites using Fe3O4 nanoparticles and a 68Ga radioisotope that can be used for therapy in hypothermia cases and for diagnoses in the fields of PET and MRI applications

4. Development of shield case for 68Ge/68Ga generator
(출처: Summary 9p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 요약문 ... 4
• SUMMARY ... 8
• 표목차 ... 12
• 그림목차 ... 13
• CONTENTS ... 16
• 목차 ... 17
• 제 1 장 연구개발과제의 개요 ... 18
• 제 1 절 연구개발의 필요성 ... 18
• 제 2 절 연구개발의 목표 및 범위 ... 21
• 제 2 장 국내외 기술개발 현황 ... 23
• 제 1 절 국내외 기술 개발 현황 ... 23
• 제 2 절 기술개발 현황 비교 ... 30
• 제 3 장 연구개발 수행 내용 및 결과 ... 32
• 제 1 절 68Ge 생산 표적 개발 및 68Ge 생산 ... 32
• 제 2 절 68Ge 분리 및 발생장치용 흡착재 개발 ... 53
• 제 3 절 68Ga 활용 방사성의약품 개발 ... 63
• 제 4 절 발생장치 차폐체 개발 ... 83
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 85
• 제 1 절 연구결과의 목표 달성도 ... 85
• 제 2 절 관련 분야에의 기여도 ... 86
• 제 5 장 연구개발결과의 활용계획 ... 88
• 제 6 장 참고문헌 ... 89
• 끝페이지 ... 91