초록

○ 목표 탄소수의 지방산 생산성 증진을 위한 배양 인자 2가지 영향 비교 (온도, 배지)
○ 지방산 생산 증대를 위한 배양 조건 확립 (지방산 생산 최대 37% 증진)
○ 발효기를 이용한 지방산 대량 생산 조건 확립 (온도, 배지 등 배양 조건)
○ 총 지방산량 최대 24% 증가
○ 지방산 고생산성 균체의 대량 확보 및 fed-batch 배양을 통한 고농도 배양 최적화
○ 총 지방산량 최대 7.1배, 불포화지방산량 최대 11.6배, 목적 탄소수의 지방산 양 최대 3배 증진
(출처:요약서 3p)

Abstract ▼

Ⅱ. The goal and necessity of research and development
The overall objective of the research proposal is to develop the original technology for alternative fuel production of fatty acid for efficient production of environment friendly bioenergy from biomass. In previous stage, we have developed su

Ⅱ. The goal and necessity of research and development
The overall objective of the research proposal is to develop the original technology for alternative fuel production of fatty acid for efficient production of environment friendly bioenergy from biomass. In previous stage, we have developed successfully E. coli strains of which the genes of the initiation, elongation, and termination were metabolically engineered for the efficient production of fatty acids. The objective of the last stage of this research is to develop high productivity and low cost production process of fatty acid by using metabolically engineered E. coli.
Domestic energy market depends mostly on foreign fossil fuel, and the price is rising due to the increase in the use of energy and limits of fossil fuel resources. The world economic growth and chemicals produced from fossil fuel results in serious environmental crisis. The generation of green house gas from manufacturing process causes climate change. As a solution of climate change problem, highly efficient alternative fuel production strains of fatty acid were developed. These strains were improved in fatty acid production by improving metabolic pathway of fatty acid synthesis and production mechanism.
In this research, we are planing to develop environment friendly production technology of alternative fuel of fatty acid by using the highly efficient fatty acid producing E. coli strains. The following research will be performed at the last stage of the research.

Ⅲ. Content and results of performed research and development
○ Research on the expression of proteins for fatty acid synthesis for the increase of fatty acid production

○ Optimization of the combination of vector systems that contain different groups of genes in fatty acid synthesis

○ Efficient production of free fatty acid

○ Culture optimization for the improvement of fatty acid composition and contents

○ Optimization of media composition and fermentation process for mass production of fatty acid

○ High cell density culture of highly productive strains of E. coli for mass production of the cell

These research will develop original platform technology for the production of fatty acid for efficient production of environment friendly bioenergy from biomass.

Ⅳ. Expected contribution of performed research and development
This study has a significance by suggesting a new possibility to overcome energy price increase and climate change by developing highly efficient strain for fatty acid line for the production of environment friendly alternative fuel and low cost high density cell culture technology. Energy competitiveness can be secured by developing technology essential for the production of biofuel through understanding of biosynthesis metabolic pathways using metabolic engineering of bioenergy production strain and by preoccupying technologies for developing environment friendly alternative energy based on material technology. The increase in bioenergy production by the development of fatty acid production strains and low cost, high density fermentation process can provide self-sufficiency of domestic energy.
(출처:SUMMARY 6~7p)

목차 Contents

• 표지 ... 1제출문 ... 2보고서 요약서 ... 3요약문 ... 4SUMMARY ... 6CONTENTS ... 8목차 ... 9제1장 연구개발과제의 개요 ... 10 1절 연구개발의 목적 ... 10 2절 연구개발의 필요성 ... 10 3절 연구개발의 범위 ... 13제2장 국내외 기술개발 현황 ... 15 1절 국외 동향 ... 15 2절 국내 동향 ... 17 3절 국내외 연구 및 특허 현황 ... 20제3장 연구 개발 수행 내용 및 결과 ... 22 1절 지방산 생산 균주의 성능 향상을 위한 벡터시스템 조합 최적화 ... 22 2절 효율적인 유리 지방산 생산을 위한 조건 확립 ... 28 3절 지방산계열 대체연료 생산 증대를 위한 지방산 생합성 단백질 발현 연구 ... 37 4절 목표 탄소수의 지방산 생산성 증진을 위한 배양 최적화 ... 45 5절 지방산 대량 생산을 위한 배지 및 발효공정의 최적화 ... 48 6절 지방산 고생산성 균체의 대량 확보를 위한 고농도 배양 및 최적화 ... 50 7절 연구개발성과 ... 55제4장 목표달성도 및 관련분야에의 기여도 ... 56제5장 연구개발성과의 활용계획 ... 58 1절 연구개발결과의 활용방안 ... 58 2절 기대효과 ... 58제6장 연구개발과정에서 수집한 해외과학기술정보 ... 60 1절 학계 과학기술 정보 ... 60 2절 산업계 바이오연료 연구 ... 62제7장 연구시설ㆍ장비 현황 ... 65제8장 참고문헌 ... 66끝페이지 ... 68