초록 ▼

- 혈액채취를 위한 마이크로니들의 구조 설계 및 제작(최종의 구조: 길이 2000 ㎛, 내경)
- 기능성 파릴렌 코팅의 특성평가 (두께: 100 ㎚이하, 평편도: < 50 ㎚)
- 채널의 표면 친수성, 유체 거동제어, 유속 분석
- 극미량 혈액 전처리 및 신호 증폭이 가능한 적충형 필름 박막을 개발함
- 초고감도 특이적 항체를 선별할 수 있는 기술을 개발함.
- 표지 물질 감지용 항체 고정화 기술 개발
- 초고감도 면역 센서의 개발
- 미소전극에 초고감도 면역 센서의 적용
- 고감도 신호감

- 혈액채취를 위한 마이크로니들의 구조 설계 및 제작(최종의 구조: 길이 2000 ㎛, 내경)
- 기능성 파릴렌 코팅의 특성평가 (두께: 100 ㎚이하, 평편도: < 50 ㎚)
- 채널의 표면 친수성, 유체 거동제어, 유속 분석
- 극미량 혈액 전처리 및 신호 증폭이 가능한 적충형 필름 박막을 개발함
- 초고감도 특이적 항체를 선별할 수 있는 기술을 개발함.
- 표지 물질 감지용 항체 고정화 기술 개발
- 초고감도 면역 센서의 개발
- 미소전극에 초고감도 면역 센서의 적용
- 고감도 신호감지 및 구동 제어 기술
- 센서 Data 처리 기술
- 비교검사용 serum 및 citrated plasma 검체 확보
(출처 : 보고서 초록 3P)

Abstract ▼

Ⅳ. Results
1) Minimally invasive, non-biofouling blood-extraction technology using microneedles
○ Using the new "Drawing Lithography" technology fabricated various inner-diameter(40~80㎛) and lengths (500~2000㎛) of hollow microneedle for subcutaneous blood extraction without causing pain. In ad

Ⅳ. Results
1) Minimally invasive, non-biofouling blood-extraction technology using microneedles
○ Using the new "Drawing Lithography" technology fabricated various inner-diameter(40~80㎛) and lengths (500~2000㎛) of hollow microneedle for subcutaneous blood extraction without causing pain. In addition, the single type and multi-array type of hollow micorneedles was fabricated with physical strength for insertion into skin without breaking risk.
○ High deformation PDMS (polydimethylsiloxane) was usded to fabricated the blood extraction device (height 11 mm; width 11 mm). This simple elastic self-recovery actuator converts finger force into elastic energy in the form of a PDMS switch to power blood extraction and transport blood without requiring an external power source. We used this novel device successfully to extract and transport blood from a rabbit.
○ Less than 50 ㎚ thickness of parylene coating can perform through controlling functional parylene under QCM technique for the deposition. Parylene thin film micro-needle and applied to the surface of biocompatible Granted.
○ This technology the PDMS pump can integrate in the device without losing the hydrophilic surface. The result of DNA separation using the device was published in Lab on a Chip (2011, 11, 348-353). This hydrophilic PDMS microfluidic devices equipped with a water contact angle of 50 degrees under the capillary action. Using this device the extracted blood sample can be transport inside of the hydrophilic microchannel few cm per minute.
2) Blood pretreatment, highly sensitive antibodies, and antibody immobilization
○ Application to ACS monitoring device at home and work place
○ Application to acute or chronic disease monitoring biosensor
○ Application for environment, food, national defence, scientific investigation, etc.
○ Application for U-health care, environmental monitoring part in the future
3) Highly sensitive multiplex immunosensing for acute cardiovascular diseases
○ Miniaturized reference electrode for microchip
- Fabrication of solid-state miniaturized reference electrode by combining nanoporous Pt
○ Selective immobilization of biomolecules using electrochemical method
○ Fabrication of three-dimensional interdigitated array electrode
- Optimal electrode structure condition for electrochemical signal amplification
○ Signal amplification for high sensitivity
- Signal amplification using redox cycling
- Enhanced reproducibility in signal amplification and ulrtasensitive detection
○ Development of an ultrasensitive electrochemical biosensor
- Ultrasensitive electrochemical biosensors employing a reducing agent
- The best conditions for overall performance including fluidics, volume, reaction time, temperature etc.
4) Signal measurement/processing system for body-attachable diagnostic system
○ Conceptual design of system integration
- Each unit device and system specification of all of the sensor module and user requirement decision
- Key processing development for packaging of each unit device
○ Definition of specification of high sensitivity signal detection
- Requirement analysis and standard definition
- High sensitivity signal detection and biosensor drive control specification decision
- Mesurement and manufacure of signal precess part
○ Definition of specification of sensor data precess
- Definition of specification of sensor data precess
- Analysis of basic data
- Signal processing front/end interface
○ Mesurement of bio-chemical sensor layer
- Mesurement of proto-type bio-chemical according to packaging of each unit device
○ High sensitivity detection and drive control technique
○ Sensor data signal processing technique
- Sensor data signal processing, analysis, save and transmission technique
- Signal processing technique
- Software technique for sensor electrochemical measurement
5) Protocols for clinical trials associated with biomarker-based disease diagnosis
○ Four hundred serum samples for troponin T and 751 citrated plasma samples for tropoin I were collected after the approval of institutional review board.
○ Two hundred seventy nine patients who performed coronary angiogram were tested for plasma MPO, troponin T, troponin I levels. Study patient were classified into 4 categories, Normal, 1 vessel disease, 2 vessel disease, 3 vessel disease according to coronary angiogram. Troponin I and troponin T were higher in 2 vessel and 3 vessel disease, which was statistically significant.
○ Plasma MPO level was measured for the 73 patient, admitted with chest pain. MPO level was significanly aid the differentiation of acute myocardial infarction from other disease category.
○ Plasma MPO level was not increased in patient with instent restenosis (ISR) compared to patient without ISR. However, high sensitive CRP level does discriminate the presence of ISR.
○ Standard DARC phenotypic method was developed, and performed with 516 Koreans. Phenotypic distribution was Fy(a+b-) 87.2%, Fy(a+b+) 12.0% and Fy(a-b+) 0.8%. Especially in case of Fy(a+b+), 32% showed weak expression of Fyb antigen.
○ Unique SNP (single nucleotide polymorphism) in Korean was founded, such as 3’-UTR 250C>T, IVS1 235 T/deletion, exon 2 199C>T by DARC sequencing analysis.
(출처 : SUMMARY 8P)

목차 Contents

• 표지 ... 1제 출 문 ... 2보고서 초록 ... 3요 약 문 ... 4SUMMARY ... 8CONTENTS ... 11목차 ... 12제1장 연구개발과제의 개요 ... 13 제1절 연구개발의 목적 ... 13 제2절 필요성 ... 14제2장 국내외 기술개발 현황 ... 16 제1절 국내외 기술 수준 ... 16 제2절 국내외 연구 현황 ... 23 제3절 국내외 연구개발실적 ... 28 제4절 국내외 기술개발현황에서 차지하는 위치 ... 38제3장 연구개발 수행 내용 및 결과 ... 40 제1절 연구개발 추진 전략·방법 및 추진체계 ... 40 제2절 연구개발 수행내용 및 결과 ... 44 제3절 핵심기술 개발 실적 ... 66 제4절 연구 성과 ... 73제4장 목표달성도 및 관련분야에의 기여도 ... 85 제1절 연구개발 목표 ... 85 제2절 연구개발 목표(평가착안점) 대비 달성도 ... 85 제3절 연구단 목표 및 관련분야 기여도 등 ... 88제5장 연구개발결과의 활용계획 ... 92 제1절 개발기술의 활용계획 및 기대효과 ... 92 제2절 추가연구의 필요성 ... 95제6장 연구개발과정에서 수집한 해외과학기술정보 ... 96제7장 참고문헌 ... 98끝페이지 ... 106