Abstract ▼

□ Purpose&contents
Increased expression of osteopontin (OPN) has been reported in atherosclerotic lesion as well as in the vasculatures of high risk groups of proliferative vascular diseases including diabetes and hypertension, suggesting that OPN is closely related to the development of vascular

□ Purpose&contents
Increased expression of osteopontin (OPN) has been reported in atherosclerotic lesion as well as in the vasculatures of high risk groups of proliferative vascular diseases including diabetes and hypertension, suggesting that OPN is closely related to the development of vascular diseases. To develop therapeutics for proliferative vascular diseases, the following will be investigated in this study: (1) OPN expression and involved mechanisms, and its role in vascular cells, (2) Identification of transcription factors involved in OPN expression, and (3) their role in proliferative vascular diseases.

□ Result
● 1st Year Results: Identification of the role and related mechanisms involved in OPN expression in vascular cells
1. Increased expression of OPN in diabetic and hypertensive vasculatures was observed. Using in vitro model of diabetes and hypertension, the molecular mechanisms involved in OPN expression in VSMC was identified.
2. The role of OPN and related signals involved in MMP production in VSMC were identified. Moreover, the role of OPN in MMP production was determined using OPN-deficient mice.

● 2nd Year Results: Identification of transcription factors involved in OPN expression in vascular cells
1. OPN reporter construct containing various size of OPN promoter was manufactured, and then identified AP-1 and CEBP-beta as potential transcription factors mediating OPN expression in diabetic and hypertensive vasculatures.
2. The transcription factors mediating OPN expression were identified using mutant construct, gene regulation and ChIP analysis.

● 3rd Year Results: Identification of the relationship between target transcription factors and proliferative vascular diseases
1. The transcription factors and related signals mediating OPN expression in VSMC in response to various stimuli including oxygen free radicals, mechanical stretch and growth factors, were identified. In addition, the relationship between intimal hyperplasia, OPN and target transcription factors was determined.
2. Alpha-Isocubebene as a regulator for OPN expression and cell proliferation induced by growth factors was discovered.

□ Expected Contribution
This study will provide novel basis for the development of therapeutics through identification of novel molecules regulating target molecules involved in the development of proliferative vascular diseases;

1. Obtain patents and establishment of scientific basis related to the development of new drugs for cardiovascular diseases.
2. Application for molecular therapy and target-based therapeutics for proliferative cardiovascular diseases.
3. Application for therapeutics for various cell proliferative diseases.

( 출처 : SUMMARY 5p )

목차 Contents

• 표지 ... 1목차 ... 2연구계획 요약문 ... 3연구결과 요약문 ... 4 한글요약문 ... 4 SUMMARY ... 5연구내용 및 결과 ... 6 1. 연구개발과제의 개요 ... 6 2. 국내외 기술개발 현황 ... 8 3. 연구수행 내용 및 결과 ... 11 4. 목표달성도 및 관련분야에의 기여도 ... 37 5. 연구결과의 활용계획 ... 38 6. 연구과정에서 수집한 해외 과학기술정보 ... 38 7. 주관연구책임자 대표적 연구실적 ... 39 8. 참고문헌 ... 40 9. 연구성과 ... 41 10. 국가과학기술지식정보서비스에 등록한 연구시설‧장비 현황 ... 48 11. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 48 12. 기타사항 ... 49별첨1 ... 50별첨2 ... 63끝페이지 ... 63