초록 ▼

본 연구진은 1단계에서 제대혈, 지방, 양막 유래의 중간엽 줄기세포를 분리하여, 최적의 분리 배양법을 확립하였으며, 이들 줄기세포를 이용하여 신경세포 분화 유도방법을 개발하였음. 또한 숙주면역세포와 줄기세포의 효율적인 공배양법확립과 줄기세포의 면역조절 cytokine과 관련 receptor의 발현여부 확인하였음. 한편 두부 및 척추신경의 손상에 따른 줄기세포의 효능을 확인하기 위해 Turbo GFP expression vector를 포함하는 lentivirus를 제작하여 제대혈, 지방 유래 중간엽 줄기세포에 성공적으로 표지하였으며,

본 연구진은 1단계에서 제대혈, 지방, 양막 유래의 중간엽 줄기세포를 분리하여, 최적의 분리 배양법을 확립하였으며, 이들 줄기세포를 이용하여 신경세포 분화 유도방법을 개발하였음. 또한 숙주면역세포와 줄기세포의 효율적인 공배양법확립과 줄기세포의 면역조절 cytokine과 관련 receptor의 발현여부 확인하였음. 한편 두부 및 척추신경의 손상에 따른 줄기세포의 효능을 확인하기 위해 Turbo GFP expression vector를 포함하는 lentivirus를 제작하여 제대혈, 지방 유래 중간엽 줄기세포에 성공적으로 표지하였으며, 과발현되는 인자(chemokine)의 동정 및 기능분석을 실시하였음.

(출처:보고서 요약서 p.3)

Abstract ▼

IV. Results

1. Development on isolation methods and biomarkers for mesenchymal stem cells
- We developed methods of isolation, incubation and differentiation of stem cell from cord blood, fat tissue and amnions.
- We identified optimal incubation methods of mesenchymal stem cells - We d

IV. Results

1. Development on isolation methods and biomarkers for mesenchymal stem cells
- We developed methods of isolation, incubation and differentiation of stem cell from cord blood, fat tissue and amnions.
- We identified optimal incubation methods of mesenchymal stem cells - We developed the neuronal differentiation methods of stem cell
- We labeled stem cells with turbo GFP expression vector with success.
- We confirmed the differentiation of stem cells into various tissue.

2. Analysis of immune mediate response after stem cell implantation
- We elucidated the coculture system to observe the relationship between host immune cells and stem cells and identified the cytokines and its related receptors
- We conducted cytokine analysis to observe the host immune response
- We confirmed the implantation methods at various disease conditions

3. Identification and functional analysis of chemokines in brain and spinal cord injury
- We developed the brain and spinal cord injury models
- We identified and analyzed the chemokines secreted in brain and spinal cord injury
- We studied functions of chemokines secreted in vitro and in vivo models
- We isolated neural stem cells from brain and spinal cord
- We observed some specific chemokines using molecular biology techniques in mesenchymal stem cells and constructed the gene therapy using chemokines in mesenchymal stem cells

4. Construct the molecular mechanism in acute injury model
- We constructed the molecular network between exogenous and endogenous stem cells in acute injury lesions.
- We modulated the expression of REX-1, SOX-2 using shRNA and confirmed the function and mechanism of these factors in stem cells
- We analyzed the immune receptor activity based on cytokine assay after mesenchymal stem cell implantation
- We analyzed signal tranduction mechanism, intracellular calcium signaling, reactive oxygen species and phosphorylation/dephosphorylation enzyme activity in mesenchymal stem cells

5. Study on correlation between stem cells and endogenous neural precursor cells
- We confirmed the apoptosis, survival, proliferation, migration and differentiation of neural stem cells after mesenchymal cell implantation
- We confirmed the neuronal and neuroglial transdifferentiation of mesenchymal stem cells in spinal cord injured lesions
- We confirmed effects of anti-apoptosis and neurogenesis after mesenchymal cell implantation

(출처:SUMMARY p.8)

목차 Contents

• 표지 ... 1제출문 ... 2보고서 요약서 ... 3요약문 ... 4SUMMARY ... 8CONTENTS ... 12목차 ... 13제 1 장 연구개발과제의 개요 ... 14제 2 장 국내외 기술개발 현황 ... 23제 3 장 연구개발수행 내용 및 결과 ... 24제 4 장 목표달성도 및 관련분야에의 기여도 ... 122제 5 장 연구개발결과의 활용계획 ... 128 1절 추가연구의 필요성 ... 128 2절 타 연구에의 응용 ... 128제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 129제 7 장 참고문헌 ... 130끝페이지 ... 132