초록 ▼

본 연구는 산림 수종인 편백, 사스레피, 동백나무에 대해 청정추출 기술인 초임계유체추출 등의 기술을 활용한 추출/분리/정제 제형화/유효기간 연장기술을 개발하고, 안 피로 예방 및 제어 효능에 대하여 임상시험을 통해 입증하고, 본 물질에 대한 기능성 의약외품 개발과 이에 관련된 기술을 개발하기 위하여 수행하였으며, 다음과 같은 결과를 얻었음
◦ 청정추출 기술인 초임계유체추출 프로토콜을 확립하고 증기증류추출과 비교 시 높은 수율을 확인하였고, 시료의 표준화를 위해 제철 시료의 성분 분석과 산패도 측정을 통해 유효기간 연장을 기술을

본 연구는 산림 수종인 편백, 사스레피, 동백나무에 대해 청정추출 기술인 초임계유체추출 등의 기술을 활용한 추출/분리/정제 제형화/유효기간 연장기술을 개발하고, 안 피로 예방 및 제어 효능에 대하여 임상시험을 통해 입증하고, 본 물질에 대한 기능성 의약외품 개발과 이에 관련된 기술을 개발하기 위하여 수행하였으며, 다음과 같은 결과를 얻었음
◦ 청정추출 기술인 초임계유체추출 프로토콜을 확립하고 증기증류추출과 비교 시 높은 수율을 확인하였고, 시료의 표준화를 위해 제철 시료의 성분 분석과 산패도 측정을 통해 유효기간 연장을 기술을 확립하였음
◦ 난대수종 추출물들이 항산화계 효소들에 대한 높은 활성을 갖는 것으로 분석되었고, 피부자극시험과 안점막 자극시험에서 안전성이 입증됨에 따라 이러한 결과들을 토대로 안구피로회복용 소재로 산업적 활용 가능성이 높을 것으로 판단됨
◦ 난대수종 추출물의 효능을 안구세포와 동물실험 통해 항산화, 항노화 및 항염증 효과를 확인하였고 이를 근거로 개발된 수면 안대 시제품으로 임상시험연구를 실시하여 눈 피로 및 안구건조를 완화시키는 등 치료적 효능을 입증하였음
◦ 본 연구 수행 중 확립된 추출 기법 및 시료의 표준화와 생리활성물질의 효능을 과학적 근거로 눈 건강 제품 개발을 위하여 다양한 형태의 시제품 제조 구축과 디자인 및 홍보물 제작 등 여러 가지 전략을 시도하였고, 최종적으로 휴앤트리 브랜드와 함께 향 지속성이 강한 천연 추출물 수면 안대 시제품을 개발하였음
(출처 : 보고서 요약서 4P)

Abstract ▼

Ⅳ. Research and development achievement

First detailed assignment: bulk demarcation and formulation processes of Chamaecyparis obtusa, Camellia japonica, and eurya japonica extracts using clean technologies.
◦ Development of supercritical fluid extraction protocol and optimized processes

Ⅳ. Research and development achievement

First detailed assignment: bulk demarcation and formulation processes of Chamaecyparis obtusa, Camellia japonica, and eurya japonica extracts using clean technologies.
◦ Development of supercritical fluid extraction protocol and optimized processes
-Extraction rate was significantly higher compared to the most common method; steam distillation extraction

◦ Analysis of containing substances for standardization of samples
-Differences of SPE and SDE substances were noted in the autumn-harvested Chamaecyparis obtusa samples. Guaiol showed highest contents (42.02%), followed by Elemol, cembrene, terpinyl acetate according to GC/MS analysis
-Lipid rancidity analysis showed that extracts are resistant to oxidation. This result indicated that extracts are composed of unsaturated compounds, rather than saturated compounds.

◦ Development of conditions and processes for bulk extraction of index substances
-During the research, temperature (critical temperature) was fixed, and only conditions related with pressure was considered in the supercritical fluid extraction protocol. During the extraction procedure, extraction rate was increased in proportion to extraction pressure. In addition, smaller particle size led to higher extraction rate.
◦ selection of index substances
-Index substances were chosen according to the supercritical fluid extraction results.

Second detailed assignment: physiologically active substances contents and functionality analysis of Chamaecyparis obtusa, Camellia japonica, and eurya japonica
-Total polyphenol contents were quite high both in the 80% MeOH extracts and SPE extracts. Total flavonoid contents was quite higher in the 80% MeOH extracts when compared to SPE extracts. Camellia japonica extracts showed relatively high anti-oxidative property both in the SPE extracts and 80% MeOH extracts. Chamaecyparis obtusa extracts showed significantly higher anti-oxidative property in the 80% MeOH extracts when compared to SPE extracts. Anti-oxidative property of eurya japonica extracts was relatively low. Free ester, ester type phenolic acid (among bounded type ester) was detected mostly. Among those, gentistic acid and salicylic acid took the highest portion. Amino acid contents were significantly higher in th 80% MeOH extracts. Alanine, lysine, phenylalanine, glutamate, glutamine was highly detected.
-Volatile fragrance substances analysis showed several compounds detected in hexane extracts, hexane fraction.
-Safety analysis showed that no irritation was noted after Chamaecyparis obtusa and Camellia japonica extracts application in the skin irritation test and ocular irritation test.

Third detailed assignment: Ocular functionality and safety assessment of Chamaecyparis obtusa, Camellia japonica, and eurya japonica extracts

◦ Anti-oxidative, anti-aging assessment of physiologically active substances of Chamaecyparis obtusa, Camellia japonica, and eurya japonica extracts
-Concentration of the diluents of the forest resources extracts was set according to the cell viability test results. Efficacy was shown in the in vitro test of corneal epithelial cells, proven by modulation of anti-oxidative and anti-aging protein. Background mechanism of ocular functionality was proven in the industrialization aspect.
◦ Effectiveness, safety, efficacy assessment of Chamaecyparis obtusa, Camellia japonica, and eurya japonica extracts (in vivo)
-After induction of dry eye in the mice, increase of the tear secretion and tear breakup time and decrease of corneal damage and ocular surface inflammation were noted after instillation of 0.1% extract.
◦ Effectiveness, safety, efficacy assessment of Optimal mixture ratio of Chamaecyparis obtusa and Camellia japonica extracts
-In vitro test results showed that supercritical fluid extracts of Chamaecyparis obtusa and Camellia japonica have anti-oxidative property. Optimized extract mixture (Chamaecyparis obtusa : Camellia japonica = 9 : 1) showed mild (statistically not significant) increase of cell viability (no significant difference compared with sole extracts)
-After induction of dry eye in the mice, optimized extract mixture showed meaningful results in 0.1% concentration.
-Clinical trial of eye patch containing optimized extract mixture demonstrated that ocular surface disease index, eye fatigue index and tear breakup time was improved compared to the baseline value. This eye patch can be used as an adjunctive method for the improvement of symptoms and signs of dry eye patients and ocular fatigue patients.

Fourth detailed assignment: Development of ocular functional product and industrialization
◦ BI manufacture completed, spray type container and wrapping design manufacture completed.
◦ Proportion material manufacture completed.
◦ Functional eye patch for sleep using Chamaecyparis obtusa manufacture completed.
◦ Functional effect of Chamaecyparis obtusa extracts was proven.
(출처 : SUMMARY 9P)