초록 ▼

〇 참담치의 주산란기는 서해안(2월-6월)，남해안(3-7월)，동해안(5월)로 확인 되었다. 포 란량은 서해안(포란량은 348.4x10⁶ eggs), 남해안(341.5x10⁶ eggs)로 확인되었다.

〇 인공종묘생산을 위한 산란자극기술로는 담수자극이 가장 효과적이며 유생최적사 육조건은 수온(18~21℃，사육밀도(5~10개체/㎖)으로 구명되었다. 유생사육시 프로바이오틱스(생균제)를 첨가하였을 경우 고밀도사육이 가능하고 생존율이 57.2-77.6%로 약 1.5〜2배정도 향상되었다.

〇 참담치의 주산란기는 서해안(2월-6월)，남해안(3-7월)，동해안(5월)로 확인 되었다. 포 란량은 서해안(포란량은 348.4x10⁶ eggs), 남해안(341.5x10⁶ eggs)로 확인되었다.

〇 인공종묘생산을 위한 산란자극기술로는 담수자극이 가장 효과적이며 유생최적사 육조건은 수온(18~21℃，사육밀도(5~10개체/㎖)으로 구명되었다. 유생사육시 프로바이오틱스(생균제)를 첨가하였을 경우 고밀도사육이 가능하고 생존율이 57.2-77.6%로 약 1.5〜2배정도 향상되었다.

〇 참담치 초기부착치패의 안정적인 사육을 위해서 수온(18~21℃，염분(30~ 33psu), 사육밀도(2,500~5,000개체/m²)가 적당하고 중간양성은 멧목식 중간양성이 효과적인데 채롱수하식에 비하여 사육기간이 50%정도 단축되었으며 생존율도 약 20%정도 향상되는 것으로 나타났다.

〇 서해안의 축제식 양식장은 담수 유입방지와 동계 저수온에 대한 대처 방안이 마련되면 중간양성장으로 활용이 가능할 것으로 나타났다.

〇 남해안에서는 로프수하식 양성이 채롱수하식 양성보다 참담치 양성에 효율적인 것으로 나타났으며 참담치 양성에 적합한 해역이 선정된다면 산업화에 매우 효과적인 것으로 나타났다. 서해안 갯벌에서의 수평망식 양성은 참담치 양식에 적합하지 않는 것으로 나타났다.

(출처 : 보고서 요약서 3p)

Abstract ▼

Ⅳ. Result of Research and Development

○ In Korea coast, the water temperature was showed that eastern(7.5~23.7℃), western(3.3~26.2℃) and southern(11.2~25.1℃). The concentration of DO was showed eastern(6.9~9.4㎎/L), western(5.8~12.6㎎/L) and southern(6.5~10.8㎎/L). The salinity(30~34.2psu) and p

Ⅳ. Result of Research and Development

○ In Korea coast, the water temperature was showed that eastern(7.5~23.7℃), western(3.3~26.2℃) and southern(11.2~25.1℃). The concentration of DO was showed eastern(6.9~9.4㎎/L), western(5.8~12.6㎎/L) and southern(6.5~10.8㎎/L). The salinity(30~34.2psu) and pH(7.8~8.5) was not significant changed in Korea coast In western coast，DIN and DIP were showed greater variability from month to month The chlorophyll-a concentration was showed eastern(1.62㎍/L), western(4.46㎍/L), southern(㎍/L). Western coast showed large variation of chlorophyll-a concentration from month to month On the other hand southern coast showed comparatively low concentration The amount of phytoplankton showed that eastern coast has low but southern coast and western coast has more high.

○ The M. coruscus collected in eastern, southern and western coast was found differences of external form. The size of M. coruscus collected from southern coast showed very large than eastern and western coast. was large compared to the objects collected from eastern and western coast. On the other hand meat weight of M.coruscus collected from western coast showed very high level.

○ The M. coruscus inhabit Korea coast was showed M. coruscus by analysis of COI gene. The reproductive cycle identified by histological analysis.The main breeding season was showed that western coast(February~June), southern coast(March〜July) and eastern coast(May).

○ The M. coruscus inhabit western coast had the maximum value of GSI was 37.38%, and fecundity was 348.4 x 10⁶ eggs, while M. coruscus inhabit southern coast had the maximum value of GSI was 67.09% and fecundity was 341.5 x 10⁶ eggs. The condition of suitable parent shellfish was showed that western coast(3 years old,shell height:105〜110mm),southern coast(3-5 years old.shell height:140〜150mm).

○ In southern and western coast, the results of natural seedling collection possibility, the period of highest apperance of attachment stage larvae showed around the middle of April, the suitable period of seed collection showed around the middle of April to around the last of April. In case of southern coast showed subsurface seedling collection of below a depth of 10 m, in case of western coast showed rope hanging seedling collection of below a depth of 7〜8m had effective.

○ The most effective technic of spawning stimulation for artificial seedling production was showed fresh water stimulation and also, suitable culture condition for larvae was showed water temperature(18~21℃) and culture density(5~10 individual/ml)

○ Also, the filteration rate of float larvae was increased by high temperature and large size. And the food organism such as Isochrysis glbana and Teleaulax amphioxeia were showed improvement of growth and survival by a mixed feeding.

○ The addition of probiotics(Bacillus stratosphericus and Brevibacterium halotolerans) in the larval rearing was showed possibility of high density breeding, and the survival rate(57.2〜77.6%) was improvement about 1.5-2 times.

○ The artificial collection of fish vary depend on the attached substrate, a blackout airtain(1,160 ind./10㎝) showed high effect.

○ The artificial seed production suitable condition in an attempt to stable culture of early spat was a temperature(18~21℃), salinity(30~33psu) and cultural density(2,500〜5,000 ind./m²). And suitable feed organism and feed rate were revealed that Isochrysis galbana and Teleaulax amphioxeia mixed feeding as daily 5X10⁴ cell/㎖. Furthermore closing water type culture is general in order to minimized the unit production cost however our culture system was semi running water type except feeding time The semi running water type culture was showed improvement survival rate(53.2〜66.5%) as more 2 times.

○ The study investigate the gene expression of Hsp70 and GST on change of temperature and salinity in Mytilus coruscus. The expression of Hsp70 mRNA was no significant changed in M.coruscus exposed to temperature (8℃, 20℃, 30℃) and salinity (20‰, 25‰, 30‰) for 14 days. Whereas the expression of Hsp70 mRNA was increased in exposure to temperature 30℃ and salinity (20‰, 25‰, 30‰) for 28 days. The expression of GST mRNA was increased in exposure to temperature 30℃ salinity (25‰, 30‰) for 14 days and temperature (8℃, 20℃, 30℃), salinity (20‰, 25‰, 30‰) for 28 days.

○ On the other hand, the study investigate the gene expression of Hsp70 and GST during air exposure and starvation. The expression of Hsp70 mRNA was significantly changed in the experiment groups, such as air exposure and starvation. GST mRNA expression was significantly increased in the experimental group of starvation.

○ In southern coast, cage hanging intermediate cultivation showed the growth and survival rate not difference according to water depth, the growth and survival rate showed well in cultural density(500 ind./m²). However, cage hanging intermediate cultivation showed slow of growth overall, therefore, our were operated raft intermediate cultivation because of the easy management near coast.

○ In intermediate culture, the raft cultivation was spend 3 months for growth of spat shell height from 5mm to 17mm. This result showed that period was saved about 50% and survival rate was enhanced about 20% than cage hanging cultivation.

○ The survival rate and growth of spat breeding in the upwelling water tank was showed very effective at the beginning, However, the survival and growth was ineffective at spat shell height more than 10 mm due to mass mortality as features of attachment.

○ The pond cultivation was adequate for the mussles cultivated in intermediate cage farms because of stable temperature and rich feed organism except high temperature season in western coast. This is need will establish countermeasures for high temperature and low temperature.

○ In southern coast, the growth was not changed due to water depth in cage hanging cultivation. The growth and survival rate was well according to cultural density(250 ~ 500 ind./m²) in cage hanging cultivation. However, it is difficult to industrial application due to 5 month spent in order to growth(14.5 mm~25.0 mm) of shell height. Therefore, the cultivation method replace rope hanging cultivation from September 2015.

○ The rope hanging cultivation was carried out from September 2015 to August 2016, the attached spat was showed well growing such as shell height(21.4mm~62.8mm) and daily growth(0.11mm). Furthermore, the survival rate showed as 65.6% until May 2016, therefore, it showed possibility for culture industrialization. However, it was showed final survival rate is 25% because of slow growth and mass mortality due to attachment of Mytilus galloprovincialis and high temperature in the summer since May 2016.

○ In western coast, the Off-bottom cultivation appears to be the most efficient that the spat culture maximum density 1,360 ind./㎡ in low-rise of subtidal zone than the intertidal zone. The estimated to be suitable for high quality production through utilization of fallow tidal flats in western coast.

○ The result of a productivity analysis by based on production of rope hanging type was showed poor economy industrial aspects. Because of survival rate showing 25%. But, if the self-production of artificial seed, utilization by effective labor and optimal selection of culture farms for increasing survival rate prepared, it will be secure economically.

(출처 : SUMMARY 11p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 요약문 ... 5
• SUMMARY ... 10
• 목차 ... 16
• CONTENTS ... 17
• 표목차 ... 18
• 그림목차 ... 20
• List of Tables ... 25
• List of Figures ... 28
• 제 1 장 연구개발 과제의 개요 ... 35
• 제 1 절 연구개발의 목적 및 필요성 ... 35
• 제 2 절 연구개발의 내용 및 범위 ... 37
• 제 2 장 국내외 기술개발 현황 ... 40
• 제 3 장 연구개발수행 내용 및 결과 ... 42
• 제 1 절 참담치 해역별 생물학적 특성 구명 ... 42
• 제 2 절 참담치 번식생태학적 연구 ... 60
• 제 3절 자연종묘 대량 확보를 위한 기술개발 ... 86
• 제 4절. 인공종묘 대량생산 기술 개발 ... 96
• 제 5절. 초기 부착치패 사육 기술개발 ... 117
• 제 6절. 중간양성 기술 개발 ... 140
• 제 7절. 양성 기술개발 ... 158
• 제 8절. 생산성 분석 ... 188
• 제 4 장 목표달성도 및 관련분야에의 기여도 ... 191
• 제 1 절 연구개발 목표의 달성도 ... 191
• 제 2 절 관련분야의 기술발전에 대한 기여도 ... 195
• 제 5 장 연구개발 결과의 활용계획 ... 197
• 제 1 절 추가 연구의 필요성 및 타 연구에 응용 ... 197
• 제 2 절 기업화 추진 방안 ... 198
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 199
• 제 7 장. 참고문헌 ... 200
• 끝페이지 ... 205