초록 ▼

➢ 신경신호를 in-vivo 상에서 검출 가능한 ‘나노와이어 어레이 기반 지능형/관통형 신경소자’를 개발하고, 이와 관련한 핵심/보조 기술을 확보하였으며 특허 출원 59건 (국내 51건, 국외 8건), 특허 등록 39건 (국내 33건, 국외 6건)과 논문 88편을 발표하였음.

• 신경정보 감지기술:
- 신경신호전달 메커니즘 분석을 위한 고분해능 공초점/다광자 현미경을 구축하였음.
- 고분해능 이미징/분광 시스템을 이용하여 양자점 및 다양한 생체시료의 분광 이미지를 획득하였음.

• 신경신호 변조기술

➢ 신경신호를 in-vivo 상에서 검출 가능한 ‘나노와이어 어레이 기반 지능형/관통형 신경소자’를 개발하고, 이와 관련한 핵심/보조 기술을 확보하였으며 특허 출원 59건 (국내 51건, 국외 8건), 특허 등록 39건 (국내 33건, 국외 6건)과 논문 88편을 발표하였음.

• 신경정보 감지기술:
- 신경신호전달 메커니즘 분석을 위한 고분해능 공초점/다광자 현미경을 구축하였음.
- 고분해능 이미징/분광 시스템을 이용하여 양자점 및 다양한 생체시료의 분광 이미지를 획득하였음.

• 신경신호 변조기술:
- 세포 손상 없는 다채널 수직형 신경소자 array 기술을 개발하였음.
- 하이브리드 나노선 신경소자를 이용하여 신경 자극 및 제어에 관한 연구를 수행하였음.

• 신경소자 시스템기술:
- CMOS 공정으로 16 채널 인터페이스를 설계하여 신경소자를 제작하였음.
- 신경 신호를 검출하기 위한 나노 와이어 전극, 칩간의 연결을 위한 flexible PCB를 설계 및 제작하였음.

• 신경생리 분석기술:
- 신경신호 측정 시 신경세포 전달 물질이 신경세포에 미치는 영향을 연구하였음.
- 신경소자모델에서 신경조직을 배양하여 신경소자에 의한 효과를 생리학적으로 분석하였음.

( 출처 : 보고서 요약서 4p )

Abstract ▼

In today's global race for technological superiority, it is now widely acknowledged that a country’s ability to remain at the cutting edge directly impacts on its economic fate. Due to a lack of natural resources, Korea has focused its efforts on technological development and innovation to emerge r

In today's global race for technological superiority, it is now widely acknowledged that a country’s ability to remain at the cutting edge directly impacts on its economic fate. Due to a lack of natural resources, Korea has focused its efforts on technological development and innovation to emerge rapidly as an international leader in various fields such as semiconductor technology. In order to ensure technological competitiveness of Korea, we must continue to designate our priority in gathering and occupying original core technologies for creating high value-added industries.

Korea’s population is aging rapidly, and by 2025 it is forecasted that Korea will become one of the fastest aging populations on the planet, resulting in an economic and sociologic loss for the society. This problem will be confined not only to Korea, but will be global. The importance of establishing a fundamental breakthrough technology such as neuro-technology to deal with this issue is imminent.

Current trends in neurotechnology show that previous intra-disciplinary research approaches have reached a bottleneck. It is now recognized that to produce maximum synergistic effects, interdisciplinary research should be conducted, in particular within the fields of information technology (IT), nanotechnology (NT), and biotechnology (BT). Neurodevice technology uses apparatus such as electrodes to stimulate nerves rather than by pharmaceutical methods, and uses IT based software to control those stimulations. By manipulating the nervous system through neurodevices, applications include explorations into the cognition, restoration of damaged nerves, and other areas inaccessible by current technologies. According to Neuroinsights (Neuro-technology Market Report), the market-size for neuro devices is forecasted to increase annually by 21%, showing its economic potential. Although domestic technical developments pertaining to neuro-devices is elementary, taking into account Korea’s high level of expertise in IT, nano-devices, and MEMS-based bio-sensing technologies, the timing is right for Korea to invest. For developing next generation neuro-devices, we have proposed the following specific aims, which call for inter-disciplinary IT-NT-BT convergence:

1) NT-BT Sensing technology:
- Implementing a high resolution microscope and imaging technique that can probe the energy transfer mechanisms of the neural network in-vivo.
- Constructing a high-speed multi-/two-photon microscope system composed of two-axis resonant/galvano scanners and obtaining image of PC12 cells on the nanowire electrode device
- Researching on the immune reaction of mouse skin and the surface plasmon polaritons on the metallic nano structures

2) NT-IT-BT System technology
- Developing a neurodevice that can substitute for damaged nervesby imple- menting a 128 x 128 CMOS based technology and using signal detection methods and processing tools to receive and transmit stimulations.
- Implementing a neural recording processor in a 65 nm CMOS technology whose noise level is sufficiently low.
- Developing an active neural processor that not only detects neural signals but also stimulates neurons with a pulse current.
- Acquiring localized neural signals using the multi nano-wire channel con- figuration from both neurons in the brain in-vivo and cultured neurons in vitro with a high signal-to-noise-ratio (SNR).
- Preparing nanowires on both ceramic and Si substrates using Au and Pt for neural signal detection.

3) NT-IT-BT Neuromodulation technology
- Securing neuron-semiconductor nanowire surface processing techniques and developing nanowire based signal detection/transmission/activation methods for intelligent nano-bio neurodevices.
- Studying on the the growth of nanowires, fabricating nanowire neurodevices suitable for devices based on the study, and signaling of neuron cells
- Making a coupling of neuron and nanowire by culturing hippocampus on nanowire-grown substrate and characterizing their extra- and intra-cellular interface, and then fabricating neurodevices and detected an action potential of neural cells in an alive state.

4) NT-BT Physiological technology
- Investigating the compatibility of nanowires with hippocampal neurons and the structural study of the neuron–nanowire interface.
- Securing three dimensional neural cell culturing and analysis skills. To implement a patch clamp array system that will allow high volume neural organization signal analysis.
- Presenting a novel semiconductor nanowire field-effect transistor based sensing device with an intrinsic amplification mechanism.
- Measuring a voltage change along time using the vertical nanoprobe with partial etching that shows a feasibility to monitor and stimulate an intracellular signal for a long-term in a large area.

With the help of the financial support of Korea Research Foundation through Pioneer Research Center Program, we have performed a profound study of fabrication and characterization of nanowires, nanowire arrays, and neuro-devices combining nanowire electrodes and CMOS technology for sensing localized the signals from neuron cells, neural fiber bundles, and neural networks. We could also developed spatial & temporal high resolution imaging systems for observing neuron systems, coupling schemes of nanowires and neurons for studying neuron-nanowire interface, and a novel sensing device based on nanowire field-effect transistor. Utilizing the newly developed imaging systems, we could visualize and monitor the dynamic characteristics of neurons and sensory organs of mouse and zebra-fish both in vivo and in vitro. In addition, we have developed a neuro-device based on CMOS processor and detect neuro signals from cultured neuron cell and mouse brain with high signal to noise ratio. We could demonstrate the possibility for realization of intelligent neurodevice which can detect and control the neuronal signals. Furthermore, we could establish the optimized growth condition of the nano-wires and fabrication method of nano-wire electrode-chip suitable for culturing neurons and detecting electrical signals. We could fabricate the multi-channel nano-wire neuro-device system and neuron-nanowire hybrid multi-channel nanodevice for studying neuron networks. The results have fully demonstrated that nanowires are capable of detecting neural signals and can be effectively used for measuring the localized neuro-signals. The low-noise multi-channel neuro- device based on CMOS processor has shown that it is possible to modulate neuronal signals and make device implantable. Therefore, we believe that our intelligent neuro devices can be successfully utilized as neuro stimulation devices for spinal cord stimulation, deep brain stimulation, sacral nerve stimulation, vagus nerve stimulation, gastric electric stimulation etc. and neuro prosthetic deviced for cochlear implant, retinal implant, motor prosthesis, brain-computer interface, etc., in near future.

( 출처 : SUMMARY 11p )

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보 고 서 요 약 서 ... 4
• 요 약 문 ... 6
• S U M M A R Y ... 11
• C O N T E N T S ... 16
• 목차 ... 17
• 제 1 장 연구개발과제의 개요 ... 19
• 제 1 절 연구 개발 배경 ... 19
• 제 2 절 연구 개발의 목적 ... 21
• 제 3 절 연구개발의 필요성 ... 23
• 제 4 절 연구개발의 범위 ... 26
• 제 2 장 국내외 기술개발 현황 ... 31
• 제 1 절 국내외 기술 개발 현황 ... 31
• 제 2 절 본 연구결과가 국내외 기술개발 현황에서 차지하는 위치 ... 39
• 제 3 장 연구개발수행 내용 및 결과 ... 41
• 제 1 절 제 1세부 과제 (NT-BT Sensing Technology) ... 42
• 제 2 절 제 2세부 과제 (NT-IT-BT Neuromodulation Technology) ... 71
• 제 3 절 제 3세부 과제 (NT-IT-BT System Technology) ... 88
• 제 4 절 제 4세부 과제 (NT-BT Physiological Technology) ... 103
• 제 4 장 목표달성도 및 관련분야에 기여한 내용 ... 120
• 제 1 절 연구 목표 달성도 ... 120
• 제 2 절 관련분야 기술발전 기여도 ... 145
• 제 5 장 연구개발결과의 활용계획 ... 149
• 제 6 장 융합연구 환경조성 및 연구단 운영 ... 156
• 제 1 절 융합연구환경 조성 및 운영 ... 156
• 제 2 절 세부과제 간 연계성 구축 ... 161
• 제 3 절 주관기관 지원 확약 사항 이행 여부 ... 162
• 제 7 장 연구개발과정에서 수집한 해외과학기술정보 ... 164
• 제 1 절 신경정보 감지기술(NT-BT Sensing Technology) ... 164
• 제 2 절 신경신호 변조기술(NT-IT-BT Neuromodulation Technology) ... 176
• 제 3 절 신경소자 시스템기술(NT-IT-BT System Technology) ... 181
• 제 4 절 신경생리 분석기술(NT-BT Physiological Technology) ... 182
• 제 8 장 참고문헌 ... 184
• 끝페이지 ... 188