Abstract ▼

Purpose&contents
Development of new cartilage regeneration treatment technique by inducing cartilage differentiation only by physical factor
Step 1: To monitor the changes of intracellular ATP and calcium in various electrical stimulation or mechanical stimulation conditions, and thus find the

Purpose&contents
Development of new cartilage regeneration treatment technique by inducing cartilage differentiation only by physical factor
Step 1: To monitor the changes of intracellular ATP and calcium in various electrical stimulation or mechanical stimulation conditions, and thus find the conditions of physical stimulation to induce ATP/calcium oscillations by using a system that combines bioluminescence monitoring device with electric stimulation device or mechanical stimulation device.
Stage 2: To demonstrate that electrical stimulation or mechanical stimulation to induce ATP/calcium oscillations can induce chondrogenesis of stem cells without biochemical factors.

Result
1. We have constructed the system that can monitor the changes of intracellular ATP and calcium in real time under electric stimulation or mechanical stimulation by combining electrical stimulation device, mechanical stimulation device and bioluminescence monitor device.
2. We have developed the technique to induce ATP/calcium oscillations in mouse mesenchymal stem cells using the optimized electrical stimulation.
3. We have demonstrated that the optimized electrical stimulation induces prechondrogenic condensation and increases gene expression levels of chondrogenic markers such as Col2a1, Aggrecan, Sox9 and GAGs.
4. We have demonstrated that the optimized electrical stimulation increases the expression of P2X4 receptor, TGF-beta1 and BMP2 in mouse mesenchymal stem cells and that P2X4, TGF beta1 and BMP2 signalings mediate the electrical stimulation-induced chondrogenic differentiation of mouse mesenchymal stem cells.
5. We have demonstrated that the optimized electrical stimulation drives direct reprogramming of humal dermal fibroblasts into chondrocytes.
6. We have demonstrated that the optimized electrical stimulation increases the secreted levels of TGF-beta1 and IGFBP1/2 in humal dermal fibroblasts.

Expected Contribution
1. We have succeeded in developing the technology to differentiate mesenchymal stem cells into chondrocytes by using electric stimulation. Therefore, we would like to develop electric stimulation system that can efficiently produce chondrogenic cells.
2. We believe that cell therapy using electrically-induced chondrocytes will repair the damaged cartilage in the patients with degenerative arthritis .
3. Our results will contribute to the development of electrotherapy technology that can induce cartilage regeneration in patients with degenerative arthritis.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 목차 ... 2
• 연구계획 요약문 ... 3
• 연구결과 요약문 ... 4
• 한글요약문 ... 4
• SUMMARY ... 5
• 연구내용 및 결과 ... 6
• 1. 연구개발과제의 개요 ... 6
• 2. 국내외 기술개발 현황 ... 6
• 3. 연구수행 내용 및 결과 ... 6
• 4. 목표달성도 및 관련분야에의 기여도 ... 11
• 5. 연구결과의 활용계획 ... 13
• 6. 연구과정에서 수집한 해외 과학기술정보 ... 13
• 7. 주관연구책임자 대표적 연구실적 ... 14
• 8. 참고문헌 ... 14
• 9. 연구성과 ... 15
• 10. 국가과학기술지식정보서비스에 등록한 연구시설‧장비 현황 ... 17
• 11. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 17
• 12. 기타사항 ... 17
• 별첨1 대 표 연 구 성 과 ... 18
• 별첨2 세부 목표 관련 증빙 ... 28
• 끝페이지 ... 36