Abstract ▼

Purpose&contents
• Pseudomonas aeruginosa is highly resistant to airway mucus secretion that contains molecules with antimicrobial activity. Human intestine, in which V. cholerae exhibits its virulence by the secretion of cholera toxin, is an anaerobic environment.

• The goal of this study

Purpose&contents
• Pseudomonas aeruginosa is highly resistant to airway mucus secretion that contains molecules with antimicrobial activity. Human intestine, in which V. cholerae exhibits its virulence by the secretion of cholera toxin, is an anaerobic environment.

• The goal of this study is to better understand how pathogenic microbes, such as P. aeruginosa and V. cholerae interact with their target tissues during the infection process.

Specific aim I. Pseudomonas aeruginosa research
How is P. aeruginosa quorum sensing (QS) regulated under anaerobic growth condition, compared to when it grows aerobically?
• What is the molecule that is responsible for bacterial killing in NHNE cell secretion?
• How different is the level of vitamin B12 produced in P. aeruginosa grown under aerobic vs. anaerobic conditions?
• How is P. aeruginosa quorum sensing (QS) regulated under anaerobic growth condition, compared to when it grows aerobically?
• Understanding the mode of interaction between P. aeruginosa and secretions of Normal Human Nasal Epithelial (NHNE) cells and airway trachea tissue

Specific aim II. Vibrio cholerae research
• Why Cholera Toxin (CT) production is induced during anaerobiosis?
• How glucose metabolism affects V. cholerae growth-associated phenotypes?
• Molecular basis of stringent response (SR)-mediated persister formation.
• How CT production is regulated by SR in V. cholerae?

Result
• A novel siderophore system is essential for the growth of Pseudomonas aeruginosa in airway mucus (Scientific Reports, 2015, 5:14644)

• Making Pseudomonas aeruginosa sensitive to Lysozyme (FCIM, 2017)

• A single gene of a commensal microbe affects host susceptibility to enteric infection (Nature Communications, 2016)

• Network-assisted investigation of virulence and antibiotic-resistance systems in Pseudomonas aeruginosa (Scientific Reports, 2016)

Expected Contribution
• This study will broaden our understanding of (i) the virulence mechanisms of P. aeruginosa under the airway-specific condition and (ii) the mode of interaction with host airway secretions.

• Identification of the molecule with anti-pseudomonas activity in the NHNE cell secretion will help us design a new treatment strategy for airway infection.

• Since proposed studies involve researches in microbial cell biology, biochemistry, host-pathogen interactions and microbial pathogenesis, successful outcomes of this project will contribute to the development of a competitive interdisciplinary clinical microbiology research theme in Korea.

(출처 : SUMMARY 6p)

목차 Contents

• 표지 ... 1
• 목차 ... 3
• 연구계획 요약문 ... 4
• 연구결과 요약문 ... 5
• 한글요약문 ... 5
• SUMMARY ... 6
• 연구내용 및 결과 ... 7
• 1. 연구개발과제의 개요 ... 7
• 2. 국내외 기술개발 현황 ... 7
• 3. 연구수행 내용 및 결과 ... 8
• 4. 목표달성도 및 관련분야에의 기여도 ... 9
• 5. 연구결과의 활용계획 ... 9
• 7. 주관연구책임자 대표적 연구실적 ... 10
• 9. 연구성과 ... 10
• 별첨1 대표적 연구실적 ... 12
• 끝페이지 ... 21