Abstract ▼

Purpose&contents
In this study, we will develop an intelligent bioreactor system for regeneration of defected articular cartilage caused by diseases, accident or arthritis. To achieve this, we will fabricate a human knee joint model using CAD/CAM technology and develop the culture system for rege

Purpose&contents
In this study, we will develop an intelligent bioreactor system for regeneration of defected articular cartilage caused by diseases, accident or arthritis. To achieve this, we will fabricate a human knee joint model using CAD/CAM technology and develop the culture system for regeneration of articular cartilage on the fabricated knee joint model. Also, we will develop the intelligent movement system based on a continuous passive motion(CPM) device for mimicked the human knee joint movement. Finally, we will develop the control system to control and provide various kinds of mechanical stimuli environment on the articular cartilage.

Result
We developed the bioreactor which is possible to adopt various stimuli in 3D cell-culture environment for the cartilage regeneration. Knee joint model was produced by 3D-plotting technique with the actual same size and shape of the human body and bio-inspired model. To be inserted in the knee joint model, the sponge-type cylindrical scaffold was fabricated by SLUP (salt leaching using powder) method. To determine the suitability of a scaffold in various environments of bioreactor, scaffolds were carried out for mechanical strength test, CCK-8 assay and Live&Dead analysis using fibroblast cell. In addition, we confirm the possibility of the cell culture in the bioreactor. After dividing into two groups (static culture, dynamic stimulus culture) was the experiment. The result was observed for higher cell differentiation and proliferation in the dynamic stimulus group. And, it was confirmed that a good growth of cells batter intermittent stimuli zone 1 than a continuos stimuli zone2.

Expected Contribution
We was confirmed positive effect of cell growth that to give intermittent stimulus and various dynamic stimulus in the bio-mimetic of knee joint on bioreactor. Therefore, it is expected in the future provide various standards for effectively reproducing the damaged articular cartilage.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 목차 ... 2
• 연구계획 요약문 ... 3
• 연구결과 요약문 ... 4
• 한글요약문 ... 4
• SUMMARY ... 5
• 연구내용 및 결과 ... 6
• 1. 연구개발과제의 개요 ... 6
• 2. 국내외 기술개발 현황 ... 7
• 3. 연구수행 내용 및 결과 ... 12
• 4. 목표달성도 및 관련분야에의 기여도 ... 28
• 5. 연구결과의 활용계획 ... 28
• 6. 연구과정에서 수집한 해외 과학기술정보 ... 29
• 8. 연구성과 ... 30
• 9. 국가과학기술지식정보서비스에 등록한 연구시설‧장비 현황 ... 33
• 10. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 33
• 11. 기타사항 ... 33
• 끝페이지 ... 33