초록 ▼

본 과제에서는 중국 사막화방지를 위해 농임업생명공학기술을 개발하고자 사막화지역에 적합한 형질전환 산업식물 (알팔파, 포플러)을 중국 연구팀과 공동으로 개발하고 제반특성을 분석하고 중국 건조지역 현장을 위한 실용화 기반기술을 개발하여 다음과 같은 성과를 도출하였음.
1) 환경스트레스 내성 형질전환 알팔파 개발연구로 산화스트레스 유도성 SWPA2 프로모터 조절하에 AtNDPK2 유전자, codA 유전자, IbOr 유전자를 각각 과발현하는 발현벡터를 제작하여 SN알팔파, SC알팔파, SOR알팔파를 개발하여 각종 스트레스 내성을 확인

본 과제에서는 중국 사막화방지를 위해 농임업생명공학기술을 개발하고자 사막화지역에 적합한 형질전환 산업식물 (알팔파, 포플러)을 중국 연구팀과 공동으로 개발하고 제반특성을 분석하고 중국 건조지역 현장을 위한 실용화 기반기술을 개발하여 다음과 같은 성과를 도출하였음.
1) 환경스트레스 내성 형질전환 알팔파 개발연구로 산화스트레스 유도성 SWPA2 프로모터 조절하에 AtNDPK2 유전자, codA 유전자, IbOr 유전자를 각각 과발현하는 발현벡터를 제작하여 SN알팔파, SC알팔파, SOR알팔파를 개발하여 각종 스트레스 내성을 확인하고 SCI논문(3편)에 게재하였음.
2) 환경스트레스 내성 형질전환 포플러 개발연구로 SWPA2 프로모터 조절하에 codA 유전자, Yucca6 유전자를 각각 과발현 하는 SC포플러, SY포플러를 개발하여 각종 스트레스 내성을 확인하고 SCI논문(2편)에 게재하였음.
3) 또한 본 과제를 통하여 한중협력사업 추진을 위해 전문 인력양성 (중국 박사과정 5명), 신진과학학자 교류사업 3명 등을 이용한 인력교류, 건조농업 학술교류회 개최 등 한중협력네트워크를 크게 강화하였음.

(출처 : 보고서 요약서 3p)

Abstract ▼

IV. Results

1) Transgenic alfalfa plants with enhanced tolerance to environmental stress
(1) SN alfalfa plants expressing an Arabidopsis AtNDPK2 gene
○ Transgenic alfalfa (Medicago sativa L. cv. Xinjiang Daye) plants expressing an Arabidopsis AtNDPK2 gene under the control of an oxidati

IV. Results

1) Transgenic alfalfa plants with enhanced tolerance to environmental stress
(1) SN alfalfa plants expressing an Arabidopsis AtNDPK2 gene
○ Transgenic alfalfa (Medicago sativa L. cv. Xinjiang Daye) plants expressing an Arabidopsis AtNDPK2 gene under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SN plants) were successfully generated via Agrobacteria-mediated transformation and evaluated under various stress conditions. NDPK2 is mainly associated with tolerance to abiotic stresses by activating MAPK3/6 signaling in plants and influences Aux/IAA-related gene expression by regulating auxin transport (Moon et al. 2003, Choi et al. 2005).

○ SN plants showed increased growth and tolerance to abiotic stresses such as methyl viologen (MV)-mediated oxidative stress, high temperature, drought and salt stress on the whole-plant level compared to non-transformed (NT) plants. The results were published in Plant Physiol Biochem 84: 67-77 (2014).

(2) SC alfalfa plants expressing a bacterial codA gene
○ Transgenic alfalfa (cv. Xinjiang Daye) plants in chloroplasts expressing a bacterial codA gene under the control of an oxidative stress-inducible SWPA2 promoter (SC plants) were successfully generated and evaluated under various stress conditions. Glycine betaine (GB) concentrations is correlated with the extent of stress tolerance in plants. In soil bacterium Arthrobacter globiformis, choline oxidase A (codA) converts choline into GB in a single step (Rhodes and Hanson 1993, Deshnium et al. 1995, Hibino et al. 2001).

○ SC plants with increased contents of glycine betaine showed enhanced tolerance to abiotic stresses such as MV-mediated oxidative stress, drought and salt stress on the whole-plant level compared to NT plants. The results were published in Plant Physiol Biochem 85: 31-40 (2014).

(3) SOR alfalfa plants expressing a sweetpotato IbOr gene
○ Transgenic alfalfa (cv. Xinjiang Daye) plants expressing a sweetpotato IbOr gene under the control of an oxidative stress-inducible SWPA2 promoter (SOR plants) were successfully generated and evaluated under various stress conditions. Sweetpotato Orange gene (IbOr) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses (Kim et al. 2013).

○ SOR plants with increased contents of carotenoids showed enhanced tolerance to abiotic stresses such as MV-mediated oxidative stress and salt stress on the whole-plant level compared to NT plants. The results were published in PLOS One e0126050 (2015).

2) Transgenic poplar plants with enhanced tolerance to environmental stress
(1) SC poplar plants expressing a bacterial codA gene
○ Transgenic poplar (cv. Bonghwa) plants in chloroplasts expressing a bacterial codA gene under the control of an oxidative stress-inducible SWPA2 promoter (SC plants) were successfully generated and evaluated under various stress conditions.

○ SC plants with increased contents of glycine betaine showed enhanced tolerance to abiotic stresses such as MV-mediated oxidative stress, drought and salt stress on the whole-plant level compared to NT plants. The results were published in Plant Physiol Biochem 100: 75-84 (206).

(2) SY poplar plants expressing an Arabidopsis YUCCA6 gene
○ Transgenic poplar (Populus alba x P. glandulosa cv. Bonghwa) pants expressing an Arabidopsis YUCCA6 gene, involved in IAA biosynthesis and pathway and responses to environmental stress, under the control of an oxidative stress-inducible SWPA2 promoter (SY plants) were successfully generated and evaluated under various stress conditions. YUCCA6, a member of the YUCCA family of flavin monooxygenase-like proteins, is involved in the tryptophan-dependent IAA biosynthesis pathway and responses to environmental cues in Arabidopsis (Won et al. 2011, Kim et al 2013).

○ SY plants with increased contents of auxin showed enhanced growth and tolerance to abiotic stresses such as MV-mediated oxidative stress, drought stress on the whole-plant level compared to NT plants. The results were published in Plant Physiol Biochem 94: 19-27 (2015).

3) Strength of Korea-China Collaboration network
(1) Training and exchange of professional experts
○ For training of professional experts, two Ph.D scientists (Mr. Wang Zhi and Ke Qingbo) as Korean Government Scholarship Students were trained during this project. Mr. Wang Zhi joined at Institute of Soil and Water Conservation, CAS as Assistant Professor from March 2015. In Ms. Kang Le and Ms Jin Rong have been studied at KRIBB as Ph.D student of University of Science and Technology (UST) since September 2014.

○ For exchange of professional experts, as Korea-China Young Scientist Exchange Program, Dr. Li Hongbing from Institute of Soil and Water Conservation, CAS (2013 March to 2014 March) and Dr. Bian Xiaofeng from Jiangshu Academy of Agricultural Sciences (2015 May to 2016. May) joined at KRIBB, whereas Dr. Park Sung-Chul from KRIBB (2015 May to 2016 April) joined at Sweetpotato Research Institute, CAAS. Prof. Wang Wenbin (2016 January to 2016 December) from Shanxi Agricultural University and Ms. Yang Donjing (2015 October to 2016 September) from sweetpotato Research Institute, CAAS joined at KRIBB supported by Jiangshu local government and China Scholarship Council, respectively.

(2) Joint academic activities
○ Joint Workshop on Dryland Agriculture Activities between KRIBB and Institute of Water Saving Agriculture in Arid Areas in China (IWSA), NWA&FU was held at KRIBB, Daejeon on Septemer 22, 2014. KRIBB introduced recent results on industrial plants such as alfalfa, sweetpotato, poplar for sustainable development on desertification areas in China, whereas IWSA introduced its recent research activities on dry land agriculture and 111 international collaboration project for the future research collaboration.

○ For the research collaboration, Korean PI visited four times to China and discussed on the collaboration researches to develop industrial plants to cope with climate change and to combat desertification during the project.

(출처 : SUMMARY 10p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 요 약 문 ... 4
• SUMMARY ... 9
• CONTENTS ... 14
• 목차 ... 15
• 제1장 연구개발과제의 개요 ... 16
• 제2장 국내외 기술개발 현황 ... 17
• 제3장 연구개발수행 내용 및 결과 ... 19
• 제1절 형질전환 알팔파 개발 및 특성분석 ... 19
• 1. AtNDPK2 형질전환 알팔파 (SN 알팔파) ... 19
• 2. codA 형질전환 알팔파 (SC 알팔파) ... 23
• 3. IbOr 형질전환 알팔파 (SOR 알팔파) ... 27
• 제2절 형질전환 포플러 개발 및 특성분석 ... 30
• 1. codA 형질전환 포플러 ... 30
• 2. YUCCA6 과발현 형질전환 포플러 (SY포플러) ... 33
• 제3절 한중협력네트워크 강화 ... 36
• 1. 인력양성과 인력교류 ... 36
• 2. 공동 학술행사 ... 36
• 제4장 목표달성도 및 관련분야에의 기여도 ... 37
• 제5장 연구개발결과의 활용계획 ... 39
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 40
• 제7장 참고문헌 ... 41
• 끝페이지 ... 43