Abstract ▼

The purpose of this work is to develop lentiviral vector-mediated genome editing technology by establishing integrating and non-integrating lentiviral vector system encoding CRISPR/Cas9 system, that is capable to be used in vivo animal models as well as in vitro cell culture system.

In the p

The purpose of this work is to develop lentiviral vector-mediated genome editing technology by establishing integrating and non-integrating lentiviral vector system encoding CRISPR/Cas9 system, that is capable to be used in vivo animal models as well as in vitro cell culture system.

In the present study, we developed animal model system and lentiviral vector system. In order to validate the vector system, we have tried to establish in vitro and in vivo systems expressing GFP gene which encodes targeting sequences for genome editing by Cas9-RGEN system.

◦ Establishment of GFP-expressing transgenic animal system

◦ Design, production and purification of high-titer/high-purity lentiviral vectors for genome editing in the neronal tissue of animal system

◦ Establishment of stereotaxic injection system for targeted transfer of genome editing-lentiviral vectors into cortical region and hippocampus of mouse model

(출처 : SUMMARY 8p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 요 약 문 ... 4
• SUMMARY ... 7
• 제1장 연구개발과제의 개요 ... 8
• 제2장 국내외 기술개발 현황 ... 13
• 제3장 연구개발수행 내용 및 결과 ... 15
• 제1절 염색체 비삽입성 바이러스 벡터기반 생체 내 게놈편집 활용 기술 개발 ... 15
• 제4장 목표달성도 및 관련분야에의 기여도 ... 25
• 제5장 연구개발결과의 활용계획 ... 27
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 28
• 제7장 연구시설ㆍ장비 현황 ... 33
• 제8장 참고문헌 ... 33
• 끝페이지 ... 34