초록

줄기세포 전분화능 특성 및 분화 조절 기능 분석을 통해 줄기세포 분화/역분화 가역 조절 기전을 규명함

(출처 : 보고서 요약서 3p)

Abstract ▼

Ⅱ. Research aim
Reprogramming stage-specific and differentiation stage-specific mechanistic analyses of pluripotency and fate determination.
1. Isolation and functional characterization of novel fate-determining genes leading to acquisition of proprietary technologies
2. Establishment of pr

Ⅱ. Research aim
Reprogramming stage-specific and differentiation stage-specific mechanistic analyses of pluripotency and fate determination.
1. Isolation and functional characterization of novel fate-determining genes leading to acquisition of proprietary technologies
2. Establishment of protocols for regulated differentiation based on novel mechanistic insights into the reprogramming process.
3. Understanding the nature and mechanisms of reprogramming at the molecular level
Ⅲ. Research scope and contents
1. Functional analyses of ncRNA during reprogramming and differentiation
- Identification and functional characterization of miRNAs and targets involved in cellular structure reorganization during reprogramming
- Understanding metabolism of ncRNA in pluripotent stem cells.
-Dissecting the role of miRNAs in differentiation of neural crest cell, odontogenic mesenchyme, non-dental epithelium using odontogenesis model.
2. Mechanistic analyses of cellular energy metabolism during reprogramming
- Systematic analyses of transitions in gene expression and metabolic processes during reprogramming
- Understanding the role of cellular metabolic shift in reprogramming
3. The role of fate-determining genes during reprogramming
- Functional analyses of cell cycle regulators, tumor suppressors, and protoncovenes during reprogramming
- Dissection of gene expression regulation by driver genes of reprogramming process.
4. Differentiation into hematopoietic stem cells and megakaryocytes.
- Analyzing the potency for hematopoiesis and megakaryopoiesis of somatic cell type-specific and stage-specific reprogrammed cells.
- Establishing the optimized protocol for megakaryocyte production based on various parameters of reprogramming and inducing agents
5. Cell signaling in stem cell differentiation
- Activation of GPR92 and its cell signaling cascade for megakaryocytes differentiation and platelet synthesis
- Function of novel GPCRs for differentiation of hESC and reprograming of iPSC
Ⅴ. Research application
1. Discovery and novel biotools and functional genes for reprogramming leading to patents and other applications.
2. Acquisition of proprietary technologies for regulated reprogramming based on novel genes and chemicals.
3. Application in drug discovery and drug safety and efficacy tests of stem cells based on the self-renewal and pluripotency. This is a key area of interest for global pharmaceuticals and technology transfer is a likely outcome.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 요 약 문 ... 4
• SUMMARY ... 5
• CONTENTS ... 6
• 목차 ... 7
• 제1장 연구개발과제의 개요 ... 8
• 제2장 국내외 기술개발 현황 ... 14
• 제3장 연구개발수행 내용 및 결과 ... 20
• 1 세부 ... 22
• 2 세부 ... 37
• 3 세부 ... 56
• 4세부 ... 74
• 5 세부 ... 84
• 제4장 목표달성도 및 관련분야에의 기여도 ... 94
• 1세부 ... 94
• 2 세부 ... 100
• 3 세부 ... 102
• 4 세부 ... 104
• 5 세부 ... 107
• 제5장 연구개발결과의 활용계획 ... 111
• 제6장 연구개발과정에서 수집한 해외과학기술정보 ... 115
• 제7장 연구시설ㆍ장비 현황 ... 117
• 제8장 참고문헌 ... 117
• 끝페이지 ... 120