초록 ▼

바이오매스 생산성이 우수한 미세조류를 고밀도 연속 순환 배양 방식의 최적화 기술로 바이오매스 생산 시설유지비용, 설치비용이 크게 감소 가축분뇨의 혐기성소화에서 발생하는 소화액을 사용한 새로운 수입원을 제시하여 가축분뇨 에너지화 사업의 경제성을 제고하고, 농촌의 환경사업 수익모델 창조하며 농촌 기반 지자체의 바이오-환경-에너지 융합 사업화를 통해 생산과정에서 발생하는 다양한 바이오 연료 생산 및 사료, 비료, 유용물질 등의 부가 산업을 연계 가능함. 새로운 미세조류 균주에 대하여 정성적, 정량적 지질 분석을 통하여 북극 담수 미세조류

바이오매스 생산성이 우수한 미세조류를 고밀도 연속 순환 배양 방식의 최적화 기술로 바이오매스 생산 시설유지비용, 설치비용이 크게 감소 가축분뇨의 혐기성소화에서 발생하는 소화액을 사용한 새로운 수입원을 제시하여 가축분뇨 에너지화 사업의 경제성을 제고하고, 농촌의 환경사업 수익모델 창조하며 농촌 기반 지자체의 바이오-환경-에너지 융합 사업화를 통해 생산과정에서 발생하는 다양한 바이오 연료 생산 및 사료, 비료, 유용물질 등의 부가 산업을 연계 가능함. 새로운 미세조류 균주에 대하여 정성적, 정량적 지질 분석을 통하여 북극 담수 미세조류 ArF0004, ArF0006, ArF0022, ArF0024, ArF0032가 고농도 지질함유 균주로 선발을 통하여 저온에서도 바이오매스를 생산할 수 있음.

(출처 : 요약서 3p)

Abstract ▼

Purpose & Contents
-Development of microalgal biomass which purifies piggery wastewater anaerobic digestates environmentally friendly for bio-gasification plant and microalgal biodiesel production process

- Commercialization of bio-diesel using automatic high-cell-density microalgae re-cul

Purpose & Contents
-Development of microalgal biomass which purifies piggery wastewater anaerobic digestates environmentally friendly for bio-gasification plant and microalgal biodiesel production process

- Commercialization of bio-diesel using automatic high-cell-density microalgae re-culturing system and applications of its by-products

- Technology development for producing low quality agro-livestock water for microalgae cultivation through anaerobic digestion and physicochemical processes for ammonia, color, COD reduction.

- In this study, Development and optimization of technologies anaerobic digestion process for, its post-treatment methods, and microalgae cultivation process.

- Analyses of physiology and fatty acid contents of ArM0029 and other microalgal candidates producing high amounts of the lipids in a wide range of growth temperatures and salt concentrations

- Optimization of growth media and analyses for genetic information, cellular metabolites and lipidomics of the microalgal candidates showing high concentration of the lipids formation

- Functional analyses of fatty acid desaturases in E. coli , yeast or mesophilic green microalgae by introducing recombinant DNAs

Results
- Development of microalgae delivering high productivity of biomass

- Development of an optimized technology for high-cell-density microalgae reculturing process

- Minimization of environmental pollution due to reduced water use and reuse of purified anaerobic digestion water

- Reduction of facility maintenance and installation costs through the miniaturization using living biomass circulation culture system

- Anaerobic digestate of piggery wastewater was presented as a new source of revenue. And it has created a new business model of rural business environment.

- Through a stable anaerobic digestion of piggery wastewater renewable energy was producing and the pollution load was reduced.

- It provided the anaerobic digestate for microalgae cultivation.

- It solved the cost and public complaints that occur in the process of recycling the digestate of piggery wastewater.

- It proposed possible to link the biofuel production, animal feed, fertilizer and useful materials.

- It may be possible as a means of increasing the income of rural.

- The optimal growth temperature of ArM0029 ranged from 4 to 12 ℃ and it was found that freshwater media was the optimal media.

- Qualitative identification of bright yellow colors of lipid bodies in ArM0029 cells by Nile Red staining. Quantitative analysis of C16:0 (palmitic acid), C18:1 (oleic acid), C18:3 (linolenic acid) as high concentration of fatty acids in ArM0029 by gas chromatography.

- The LC-MS analysis of ArM0029C which showed five times of phospholipid, diglyceride and triglyceride compared to those of mesophilic Chlamydomonas sp. as the control microorganism.

- ArF0004, ArF0006, ArF0022, ArF0024 and ArF0032 were selected as good candidates based on fatty acid contents and yield by qualitative and quantitative assessment in KOPRI culture collection of polar microorganisms.

- Tris base, ammonium chloride, magnesium sulfate and acetic acid showed negative effects on the ArF0024 growth whereas calcium chloride, potassium phosphate showed the positive effects.

- Negative effect of potassium phosphate and trace elements, and positive effect of ammonium chloride, magnesium sulfate on the ArM29C growth under 0.05 of significance

- The analysis of kyoto encyclopedia of genes and genomes (KEGG) pathway of overall metabolism related to the genes for fatty acid enzymes obtained from next-generation sequencing (NGS) method

- Identification of high concentration of diglycerols and triglycerols capable of being converted to the fatty acids in the culturable temperatures of ArF0024 and ArM0029C

- Collection of 55,894 and 8,947 contigs in ArF0024 and ArM0029C by next-generation sequencing method, respectively. Selection of more than 250 genes related to the fatty acid synthesis for ArM0029C

- Identification of the fatty acid desaturase (AChFAD6) that converts C18:1 (oleic acid) to C18:2 (linoleic acid) from the genetic database of ArF0006. AChFAD6 encoding 424 amino acids (48.2 kDa of predicted molecular weight) increased over three times of C18:2 fatty acids in the induction of AChFAD6 in Escherichia coli compared to the negative control (vector-only Escherichia coli )

- Modification of enzymatic activity of AChFAD6 by site-directed mutagenesis on amino acid substitution in transmembraneous region which confer the ability to convert C18:0 (stearic acid) from C18:1 (oleic acid)

Expected Contribution
- Utilizing renewable energy to build a biodiesel by product utilization and commercialization based through recultivation of high-density automatic system of microalgae

- Microalgae build biomass and biodiesel production process that can be purified to a sustainable livestock manure anaerobic digestive juice

- Using combined physicochemical processes, continuously generated livestock wastewater can be treated in a short time.

- Using treated anaerobic digestion solution, it is possible to cultivate microalgae and produce biodiesel from cultivated microalgae.

- Selection of a good microalgal candidate ArM0029 for clarification of the anaerobic digested liquids and for production of the high concentration of the lipids.

- Characterization of the physiological characteristics of ArM0029 that show high growth rate in the temperature range of 4 -12 ℃ and relatively high concentration of the lipids in the microalgal cells

- Developing microalgal candidates showing high growth rate and diverse range of the salt concentration analyzed by qualitative and quantitative methods in order to clarify the digestion and produce the lipids

- Possibility of massive production of target fatty acids in recombinant systems in various types of hosts such as E. coli , yeast and algae

- The intentional modification of fatty acid enzyme activity by site-directed mutagenesis based on protein structure of fatty acid related genes

(출처 : SUMMARY 7p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국문 요약문 ... 4
• SUMMARY ... 7
• CONTENTS ... 10
• 목차 ... 11
• 제 1장. 연구개발과제의 개요 ... 12
• 1절. 연구개발 목적 ... 12
• 2절. 연구개발의 필요성 ... 12
• 3절. 연구개발 범위 ... 29
• 제 2장. 국내외 기술개발 현황 ... 31
• 1절. 국내현황 및 문제점 ... 31
• 2절. 해외현황 및 기술개발 동향 ... 40
• 3절. 국내외 미세조류를 이용한 바이오연료시장 현황 및 전망 ... 47
• 제 3장. 연구수행 내용 및 결과 ... 54
• 1절. 연구수행내용 및 결과 ... 54
• 제 4장. 목표달성도 및 관련분야 기여도 ... 213
• 1절. 1차년도 ... 213
• 2절. 2차년도 ... 214
• 3절. 3차년도 ... 215
• 제 5장. 연구 결과의 활용 계획 ... 216
• 1절. 연구개발 결과의 활용 방안 ... 216
• 2절. 기대성과 ... 217
• 제 6장. 연구과정에서 수집한 해외과학기술정보 ... 220
• 제 7장. 연구개발과제의 대표적 연구실적 ... 229
• 제 8장. 참고문헌 ... 234
• 끝페이지 ... 238