초록 ▼

개발 목적 및 필요성
난분해성/발암성 유해오염물질인 다환 방향족 탄화수소 (PAHs) 중 B[a]P가 폐침목 사용 부지 내 조사항목에 포함되어 B[a]P 오염토양 복원 기술 개발

연구개발결과
헤모글로빈과 과산화수소 생촉매 반응 기작 규명과 생촉매 반응 후 생물학적 융합공정을 통하여 유해오염물질 B[a]P 오염토양 복원 기술을 위한 파이롯 실용화 공정개발

성능사양 및 기술개발 수준
원천기술로 B[a]P 처리를 위한 실용화를 위한 B[a]P 처리농도 0.7mg/kg 달성

활용계획 <

개발 목적 및 필요성
난분해성/발암성 유해오염물질인 다환 방향족 탄화수소 (PAHs) 중 B[a]P가 폐침목 사용 부지 내 조사항목에 포함되어 B[a]P 오염토양 복원 기술 개발

연구개발결과
헤모글로빈과 과산화수소 생촉매 반응 기작 규명과 생촉매 반응 후 생물학적 융합공정을 통하여 유해오염물질 B[a]P 오염토양 복원 기술을 위한 파이롯 실용화 공정개발

성능사양 및 기술개발 수준
원천기술로 B[a]P 처리를 위한 실용화를 위한 B[a]P 처리농도 0.7mg/kg 달성

활용계획
PAHs와 TPH 오염토양의 On situ 또는 Ex situ 정화 기술 및 난분해성 유해물질 오염토양 정화기술에 활용

( 출처: 요약서 3p )

Abstract ▼

Ⅳ. Results
1. During the biocatalytic degradation using hemoglobin and hydrogen peroxide,5-ASA was degraded by continuous non-specific catalytic reactions. We showed that this non-specific catalytic mechanism is similar to that of the reactions catalyzed by lignin peroxidase, which is produced by

Ⅳ. Results
1. During the biocatalytic degradation using hemoglobin and hydrogen peroxide,5-ASA was degraded by continuous non-specific catalytic reactions. We showed that this non-specific catalytic mechanism is similar to that of the reactions catalyzed by lignin peroxidase, which is produced by white rot fungi.

2. Continuous biocatalytic reaction mechanism can be applied to treat recalcitrant contaminants. We demonstrated that heme in hemoglobin is the key to the biocatalytic reaction mechanism.

3. B[a]P removal of 60-70% was achieved within one day, and the two optimal doses of hemoglobin and hydrogen peroxide were identified
- 0.02 g hemoglobin/2 g soil and 0.04 g hydrogen peroxide/2 g soil,
- 0.045 g hemoglobin/2 g soil and 0.1 g hydrogen peroxide/2 g soil.

4. For biocatalytic reactions in artificially B[a]P-contaminated soils without PAHs and TPH, the optimal hemoglobin dose was 0.02 g/2 g soil and the optimal hydrogen peroxide doses were 0.04-0.06 g/2 g soil. B[a]P removal of 50-70% was achieved in an hour by the biocatalytic reaction, and this reaction had about 25 times faster 1^st order reaction rate (k = 0.714/hr) than the oxidation reaction with only hydrogen peroxide.

5. The biocatalytic reaction is more efficient that the oxidation reaction with only hydrogen peroxide. Also, the decrease in the soil microorganism copy number was less and the toxicity was lower after biocatalytic reaction than after oxidation reaction with only hydrogen peroxide. Thus, there is a greater potential for biodegradation after biocatalytic reaction than after oxidation reaction with only hydrogen peroxide.

6. Various intermediate products were found during biocatalytic reaction of B[a]P with GC/MS; however, it was impossible to exactly evaluate the fate of the intermediate products.

7. The carbon mass balance of the intermediate products using ¹⁴C B[a]P showed that 0.5% of C was present as CO₂, 81% of C was present in the solvent, and 0.9% of C was present in soil. The intermediate products were converted to polar compounds in the solvent fraction.

8. The bacteria copy number after biocatalytic degradation of B[a]P was more than two times greater than that after oxidation reaction with only hydrogen peroxide. Also, the bacteria copy number after degradation of B[a]P with only hemoglobin was greater than that after oxidation reaction with only hydrogen peroxide. This implies that biological co-oxidation may take place using hemoglobin as a primary substrate.

9. From the combined biocatalytic reaction and biodegradation of B[a]P in micro-reactors, PAN reactors, and pilot systems, one day was chosen as a biocatalytic reaction period. After one day reaction, 60% TPH removal and 88% B[a]P (initial concentration of 22 mg/kg) removal were achieved.

10. After operating the Type B pilot system, the TPH, PAHs, B[a]P concentrations in the contaminated soils from the B station decreased from 6429, 392, 22 mg/kg to 10, 45, 0.7 mg/kg, respectively, after 50 days.

11. The efficiency of biocatalytic reactions using hemoglobin and hydrogen peroxide was particularly high with hydrophobic polymeric PAHs. The higher efficiency of biocatalytic reactions may be explained by increased biodegradation efficiency due to either detoxification of recalcitrant contaminants or conversion of non-polar compounds to polar compounds.

12. Therefore, the NBC-FRP process developed in this research can be applied to economically treat soils contaminated with recalcitrant hydrophobic contaminants.

( 출처 : SUMMARY 14p )

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 요 약 서 ... 3
• 요 약 문 ... 4
• SUMMARY ... 10
• 목차 ... 17
• 표목차 ... 19
• 그림목차 ... 22
• 1. 연구개발과제의 개요 ... 27
• 1-1. 연구개발 목적 ... 27
• 1-2. 연구개발의 필요성 ... 27
• 1-3. 연구개발 범위 ... 28
• 2. 국내외 기술개발 현황 ... 29
• 2-1. 국내 기술개발 현황 ... 29
• 2-2. 해외 기술개발 현황 ... 29
• 3. 연구수행 내용 및 결과 ... 31
• 3-1. 연구개발의 내용(범위) 및 최종목표 ... 31
• 3-2. 헤모글로빈과 과산화수소에 의한 생촉매 반응기작의 규명 ... 32
• 3-3. 헴과 과산화수소에 의한 촉매 반응기작 ... 44
• 3-4. 오염토양 분석 및 오염물질 분석방법 ... 51
• 3-5. 생촉매 반응 최적 투입 및 오염물질 처리 ... 69
• 3-6. 최적 헤모글로빈과 과산화수소 투여량에 의한 B[a]P 오염토양 분해 (Micro reactor 연구) ... 100
• 3-7. 생촉매 반응에 의한 B[a]P 중간물질 평가 ... 111
• 3-8. 생촉매 반응 시 14C B[a]P를 사용하여 중간물질 평가 ... 161
• 3-9. Abiotic 공정 (생촉매 반응)과 Biotic 공정 융합 처리제 ... 181
• 3-10. Pilot system 개발 연구 ... 188
• 3-11. 연구개발 결과 요약 ... 244
• 4. 목표달성도 및 관련분야 기여도 ... 246
• 4-1. 목표달성도 ... 246
• 4-2. 관련분야 기여도 ... 247
• 5. 연구결과의 활용계획 ... 248
• 6. 연구과정에서 수집한 해외과학기술정보 ... 249
• 7. 연구개발결과의 보안등급 ... 249
• 8. 국가과학기술종합정보시스템(NTIS)에 등록한 연구시설·장비 현황 ... 249
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 249
• 10. 연구개발과제의 대표적 연구실적 ... 250
• 11. 기타사항 ... 252
• 12. 참고문헌 ... 252
• 끝페이지 ... 256