연구과제
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무혈청 줄기세포를 이용하여 섬유아세포로 분화시킨후 혹은 분화되기 전의 줄기세포를 3D 프린터를 이용하여 인공피부를 제작하고 이를 실험동물 모델을 이용하여 효용성을 평가하고자 함. 그 결과 사이토카인을 확인하여 줄기세포가 분화된 것을 ...

무혈청 줄기세포를 이용하여 섬유아세포로 분화시킨후 혹은 분화되기 전의 줄기세포를 3D 프린터를 이용하여 인공피부를 제작하고 이를 실험동물 모델을 이용하여 효용성을 평가하고자 함. 그 결과 사이토카인을 확인하여 줄기세포가 분화된 것을 확인하였으며 이를 이용한 인공피부의 효능 평가에서도 효과를 입증함.

( 출처: 보고서 요약서 3p )

Abstract
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Purpose&Contents
In this study, we develop skin-regenerating scaffolds containing human mesenchymal stem cells. Furthermore, we...

Purpose&Contents
In this study, we develop skin-regenerating scaffolds containing human mesenchymal stem cells. Furthermore, we're going to evaluate safety and efficacy of the scaffolds through preclinical test, developing clinically adapted skin scaffolds.
○ Development of drug-delivery controlled cell scaffolds based on collagen-ECM
○ Development of cell-printing technique to fabricate multi-layered scaffolds including stem cells/fibroblast/keratinocyte
○ Establishment of serum-free and xeno-free stem cell culture method and GMP facility construction
○ Establishment of standard process for stem cell culture and preparation of the method of examination
○ Selection of experimental animal model and development of disease animal model
○ Efficacy evaluation of engineered skin containing stem cells using disease animal model

Results
○ Animal disease model development completed
○ Competed the development of structure for artificial skin
○ evaluation of efficiency of developed artificial skin using the animal disease model
○ Completed the development of mesenchymal stem cell into fibroblast
○ High cell viability and differentiation of the skin cells were examined due to the collagen and ECM-based bioink. The prepared bioink had proper rheological behavior for the optimal cell printing.
○ Cell printing system of more than 2 cell types with stable cell viability was developed by constructing bioprinting system supplemented with PZT actuator and core/shell nozzle.
○ Skin-regenerative cell-laden scaffold was fabricated, which had anatomical skin-mimetic structure, by fabrication of ESS (Engineered Skin Substitute) containing melanocytes, fibroblasts, and keratinocytes.

Expected Contribution
○ Practical use of clinical surgery for remedy of patients
○ Provide paradigm and tools about basic research such as cell biology, tissue biology, mechanical engineering and signal transduction mechanism
○ Especially, this study are closely connected with plenty of academics such as clinical medicine, basic science, tissue engineering, stem cell biology, mechanical engineering, which draw fusion research in the future.
○ research development, commercialization, technology manpower cultivation

( 출처 : SUMMARY 5p )

목차
Contents
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• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국문 요약문 ... 4
• SUMMARY ... 5
• 목차 ... 6
• 1. 연구개발과제의 개요 ... 7
• 1-1. 연구개발 목적 ... 7
• 1-2. 연구개발의 필요성 ... 11
• 1-3. 연구개발 범위 ... 13
• 2. 국내외 기술개발 현황 ... 15
• 3. 연구수행 내용 및 결과 ... 19
• 4. 목표달성도 및 관련분야 기여도 ... 51
• 5. 연구결과의 활용계획 ... 53
• 6. 연구과정에서 수집한 해외과학기술정보 ... 55
• 7. 연구개발성과의 보안등급 ... 56
• 8. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 56
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 57
• 10. 연구개발과제의 대표적 연구실적 ... 58
• [별첨4] 실적 증빙자료 ... 61
• 끝페이지 ... 81

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