초록

○ 벼 종자에서의 캘러스 유도를 통해서 대량 단일유전자변이체를 순수분리 하고, 전사체, 단백질체, 그리고 표현체 등의 생명정보를 이용하여 전사인자 기능연구를 통해 종자 발달 조절기작을 이해
○ 벼 생명정보 이용 저장단백질과 종자 특성 구명
○ 벼의 염 스트레스 형질 평가를 위한 이미지 정보 활용 표현형 특성 검정 체계 구축

( 출처: 보고서 요약서 4p )

Abstract ▼

□ Purpose&Contents
○ Generation of Tos17 mutant lines from callus of Ilmibyeo(IM) and Baegjinju 1ho (BJJ1ho), Korean domestic rice cultivar.
○ Deciphering gene regulatory networks in seed development through the functional analysis of transcription factors using transcropts, DNA-binding motifs

□ Purpose&Contents
○ Generation of Tos17 mutant lines from callus of Ilmibyeo(IM) and Baegjinju 1ho (BJJ1ho), Korean domestic rice cultivar.
○ Deciphering gene regulatory networks in seed development through the functional analysis of transcription factors using transcropts, DNA-binding motifs, and the phenotype of mutants.
○ Characterization of rice seed storage proteins using bioinformatics.
○ Phenomics analysis of Tos17 mutants related to salt tolerance in rice

□ Results
○ We have produced 1,778 mutants induced by Tos17 in Ilmibyeo and 842 mutants induced by Tos17 in Baegjinju 1ho and analyzed these flanking sequence tags of newly transposed Tos17 copies by the adaptor-ligation PCR method. The frequencies of Tos17 insertions in the genic and intergenic regions were 60.3% and 36.6%, respectively.
○ We also selected four Tos17 insertion mutant lines for three TF genes which can be considered to be considered to be involved in rice seed development based on expression microarray data: osrem3, osta1, osbhlh1-1, and osbhlh1-2 mutant lines.
○ According to Quadruple 9-mer-based protein binding microarray (Q9-UPBM) experiment, we found that the OsREM3, OsTA1, and OsbHLH1 bound to the ACACCAC, CACGTG, and GTAACA motifs, respectively.
○ In combination of Q9-UPBM, RiceArrayNet analysis, and expression microarray data, we identified 8, 20, and 9 putative target genes of OsREM3, OsTA1, and OsbHLH1, respectively.
○ Phenotypic Analysis of Transgenic Seed Suppressing Multiple Expression of Rice Storage Protein
○ We first generated seed storage protein (SSP)-suppressed transgenic rice plants that block the simultaneous expression of SSPs grouped in glutelin A (GluA), prolamin 13a-I and globulin using RNA interference approach.
○ For screening and characterizing the salt or drought tolerance mutations by extensive phenotypic analysis as well as the functional analysis of genes, we selected about 200 mutant lines containing 133 Tos17-inserted genes .
○ To evaluate rice phenotypic traits under abiotic stress condition, we investigated phenomics analysis, which integrates technologies such as photonics, biology, computers, and robotics.

□ Expected Contribution
○ As a result of the research, it was confirmed that Tos17 mutants could be used for early cultivation by verifying the agricultural traits such as salt resistance and high yield.
○ Characterization of major genes through regulatory network analysis related to SSPs
○ Improvement of agricultural biotechnology by controling SSPs accumulation in rice
○ Providing new insight into the role of SSPs in the formation of intracellular organelles and in germination.
○ Application of phenomics screening system for crop salt resistance test and identification of novel genes related to salt tolerance.

( 출처: SUMMARY 6p )

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 4
• 국 문 요 약 문 ... 5
• Summary ... 6
• 목차 ... 8
• 제 1 장 연구 개발 과제의 개요 ... 9
• 제1절 연구 개발 목적 ... 9
• 제2절 연구 개발의 필요성 ... 10
• 제3절 연구 개발 범위 ... 11
• 제 2 장 국내외 기술개발 현황 ... 13
• 제 3 장 연구 수행 내용 및 결과 ... 16
• 제 4 장 목표달성도 및 관련분야 기여도 ... 62
• 제1절 : 목표대비 달성도 ... 62
• 제2절 : 정량적 성과(논문게재, 특허출원, 기타)를 기술 ... 63
• 제 5 장 연구 결과의 활용 계획 ... 64
• 제 6 장 연구 과정에서 수집한 해외 과학 기술 정보 ... 64
• 제 7 장 연구 개발 결과의 보안 등급 ... 64
• 제 8 장 국가과학기술지식정보서비스에 등록한 연구시설·장비 현황 ... 64
• 제 9 장 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 65
• 제 10 장 연구개발과제의 대표적 연구실적 ... 65
• 제 11 장 기타사항 ... 65
• 제 12 장 참고문헌 ... 66
• 끝페이지 ... 68