초록

턱관절 장애 (TMJD: temporomandibular joint disorder)로 인한 악안면 통증정보의 발생, 감지, 및 전달과정에 대한 이해를 바탕으로 통증 전달 신경회로에 관련된 다양한 인자들을 전방위적으로 접근시켜 '악안면 턱관절 통증제어기술'을 구축함으로써, 악안면 내 신경 및 조직손상에 의하여 발생되는 염증성 병적통증의 진행억제 및 예방을 위한 새로운 치료 타겟 물질을 발굴

(출처 : 요약서 3p)

Abstract ▼

□ Purpose&Contents
Resolvin D2 (RvD2) are newly identified endogenous pro-resolving mediators. In the trigeminal system, we will address whether and how RvD2 can prevent and reverse temporomandibular joint (TMJ)-inflammatory pain. This application will demonstrate novel molecular and cellular mec

□ Purpose&Contents
Resolvin D2 (RvD2) are newly identified endogenous pro-resolving mediators. In the trigeminal system, we will address whether and how RvD2 can prevent and reverse temporomandibular joint (TMJ)-inflammatory pain. This application will demonstrate novel molecular and cellular mechanisms of TMJ pain and help develop new therapy for treating TMJ-inflammatory pain.
: the development of new therapeutics for TMJ-inflammatory pain using by cell penetrating-peptide based drug delivery system

□ Results
○ Specific Aim 1. Establish that local and systemic treatment of RvD2 can attenuate orofacial pain after TMJ injury in mice.
- Aim 1a: To examine whether local and systemic RvD2 injection can prevent formalin-induced spontaneous pain.
- Aim 1b: Examine whether local and systemic RvD2 can reverse CFA-induced persistent orofacial pain after TMJ injury.
- Aim 1c: Investigate whether RvD2 can inhibit non-evoked ongoing pain after TMJ injury.

○ Specific Aim 2. Define the peripheral mechanisms by which RvD2 reduces TMJ pain by modulating TRP channel function and neuronal sensitivity in trigeminal ganglion neurons.
- Aim 2a: Verification of a retrograde labeling tracer, DiI,-labeled trigeminal primary afferent neurons.
- Aim 2b: Determine whether RvD2 modulates TRPV1/A1 activity and neuronal activity of TG neurons.

○ Specific Aim 3. Define the central mechanisms by which RvD2 resolves TMJ pain by modulating excitatory synaptic transmission in the superficial dorsal horn of the trigeminal nucleus.
- Aim 3a: Determine whether RvD2 can reverse the TMJ-injury-induced synaptic plasticity in the trigeminal nucleus (Sp5C).
- Aim 3b: Determine whether RvD2 can block TRPV1-evoked enhancement in synaptic transmission in Sp5C neurons of trigeminal nuclei.

○ Published papers (SCI)
1. Improvement in Tracing Quantum Dot-Conjugated Nanospheres for In Vivo Imaging by Eliminating Food Autofluorescence. (Journal of Nanomaterials; 2015)
2. Maresin 1 Inhibits TRPV1 in Temporomandibular Joint-Related Trigeminal Nociceptive Neurons and TMJ Inflammation-Induced Synaptic Plasticity in the Trigeminal Nucleus. (Mediators of Inflammation; 2015)
3. Resolvin E1 Inhibits Substance P-Induced Potentiation of TRPV1 in Primary Sensory Neurons. (Mediators of Inflammation; 2016)
4. Unconventional Role of Caspase-6 in Spinal Microglia Activation and Chronic Pain. (Mediators of Inflammation; 2017)
5. Photodynamic therapy by conjugation of cell-penetrating peptide with fluorochrome. (International Journal of Nanomedicine; 2017)

□ Expected Contribution
• Elucidation of the biological principle for the sense.
• Development of sense control techniques for clinical and industrial application.
• Identification of mechanism-specific analgesic candidates for drug discovery.
• Technology transfer to BT industry.
• Establishment of infrastructure for the advanced sensory research.
• Core center for providing researchers with the training and education program to have expertise in sensory research.
• Contribution to improvement of the quality of life and social welfare.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 국문 요약문 ... 4
• SUMMARY ... 5
• 목차 ... 6
• 1. 연구개발과제의 개요 ... 7
• 1-1. 연구개발 목적 ... 7
• 1-2. 연구개발의 필요성 ... 7
• 1-3. 연구개발 범위 ... 10
• 2. 국내외 기술개발 현황 ... 12
• 3. 연구수행 내용 및 결과 ... 15
• 3-1. 연차별 목표 및 내용 총괄 ... 15
• 3-2. 주요 연구개발 내용 및 결과 ... 16
• 3-3. 연구개발 추진 체계 ... 23
• 3-4. 연구개발 추진 계획 ... 24
• 3-5. 연구개발 추진 일정 ... 24
• 3-6. 연차 별 연구성과 ... 25
• 4. 목표달성도 및 관련분야 기여도 ... 26
• 4-1. 목표달성도 ... 26
• 4-2. 관련분야 기여도 ... 28
• 5. 연구결과의 활용계획 ... 29
• 5-1. 연구개발 결과의 활용방안 ... 29
• 5-2 과학기술적 기대효과 및 파급효과 ... 29
• 6. 연구과정에서 수집한 해외과학기술정보 ... 31
• 7. 연구개발성과의 보안등급 ... 31
• 8. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 31
• 9. 연구개발과제 수행에 따른 연구실 등의 안전조치 이행실적 ... 31
• 10. 연구개발과제의 대표적 연구실적 ... 32
• 11. 기타사항 ... 33
• 12. 참고문헌 ... 33
• 별첨: 실적 증빙자료 ... 34
• 별첨: 기타 첨부서류 ... 44
• 끝페이지 ... 49