초록

○ 파이토케미컬 고함유 대두 계통의 품종화를 위한 기초 데이터 확보
○ 대두 성분 변화 관련 유전자 변이 정보 확보 및 발현 차이 규명
○ 대사성질환 개선용 lentil, L-Dopa 고함유 faba bean 유전자원 선발, 변이유기 및 M₂ 세대 진전
○ Faba bean 내한성 관련 유전자 탐색
○ 유용돌연변이 자원 선발, 특성 분석 및 품종보호권 출원/등록

(출처 : 보고서 요약서 3p)

Abstract ▼

Ⅳ. Results
1. Selection of soybean bred mutant lines with high-efficiency phy tochemicals
A. Development of soybean mutant bred lines with high fatty acid and oligosaccharide
- Construction of mutant pools derived from Danbaek, Daepung original soybean variety by using gamma irradiation

Ⅳ. Results
1. Selection of soybean bred mutant lines with high-efficiency phy tochemicals
A. Development of soybean mutant bred lines with high fatty acid and oligosaccharide
- Construction of mutant pools derived from Danbaek, Daepung original soybean variety by using gamma irradiation
- Selection of 10 high linolenic acid soybean mutants through investigated to agronomic traits and unsaturated fatty acid contents
- DB-HL4 (11.29%), 5 (10.88%) were significantly increased linolenic acid contents, approximately 1.7-fold higher than the Danbaek (6.53%) original variety. Also, DP-HL6 were 1.6-fold higher than the Depung original culitvar
- Construction of mutant pools derived from Paldal soybean variety
- Result of content of sucrose and oligosaccharide such as raffinose, stachyose which showed increased levels in selected mutant lines. Especially, I01 line were recorded highest sucrose content (11.81 mg/100g)
B. Development of lipoxygenase free new soybean mutant variety ‘Cheongmiwon’ and application for protection
- Development of new soybean mutant derived from hwanggum original variety by using radiation breeding, naming “Cheongmiwon” that have been application for protection (application number: 2018-118)
- new soybean mutant that exhibited lipoxygenase free phenotype

2. Analysis of functional compounds in high-efficiency phytochemical soybean bred mutant lines and Investigation of genetic variation in phytochemical related genes
A. Analysis of agronomic traits of high-efficiency phytochemical soybean bred mutant lines for variety registration.
- Construction of mutant diversity pool among 210 soybean mutant lines
- Construction of agronomic traits database
B. For compared fatty acid biosynthesis gene function, a collection of four selected soybean bred mutants with original culitvars, was used to investigate genetic variation of genomic DNA sequence and genes expression levels.
- Aligned sequence lengths of identified FAD genes relative to the Wm82 reference sequence were as follows: 2,151 bp (FAD2-1A); 1,830 bp(FAD2-1B); 1,409 bp (FAD2-2A); 2,112 bp (FAD2-2B); 2,092 bp(FAD2-2C); 2,088 bp (FAD2-2D); 4,250 bp (FAD3A); 4,104 bp(FAD3B); 2,724 bp (FAD3C) and 3,431 bp (FAD3D)
- On stage-3 expression levels, where dramatic changes were observed relative to original seeds. Transcript levels of FAD2-2B, FAD2-2D, FAD3C and FAD3D genes were significantly increased in all mutant seeds
- The highest statistically significant increased expression of FAD3C and FAD3D genes in four mutants was at stage 3, which suggests that genes were involved in linolenic acid synthesis in the selected mutants(Manuscript submitted)

3. Development of new legume cultivars for the prevention of metabolic disease through construction of mutation pool with functional foreign legume crops faba bean and lentil
A. Construction of mutation pools using radiation breeding technology and process of generation.
- Gamma-irradiated Lentil and faba bean mutant pools were generated M₂ seed pooling
- Investigation of radio-sensitivity in lentil bean to gamma-irradiation (Manuscript published)
- Selection of 10 elite faba bean individuals from 371 accessions according to agronomic traits and winter hardiness (Manuscript published)
- Faba bean mutants were exhibited 30% to 84% survival rate in winter annual

4. Establishment of the new conceptual molecular marker system and available component characteristic analysis
A. Development of marker-assisted selection system to screen in early generation
- Confirmation of DNA mutation rate in 552 M₁ faba bean mutants by using TRAP marker system
- Investigation of relationship between gamma-radiation dose and genotyping results (Manuscript submitted)
B. Analysis of functional compound contents and activity in soybean bred mutants
- Comparsion of L-Dopa, fatty acids and isoflavone content of compositae faba bean (Manuscript published)
- Result of starch physicochemical properties analysis in faba bean, these exhibited increased solubility under low melting temperature (30℃). Probably faba bean starch seems to be a novel starch category
- Soybean mutant (D-28-01) was increased 5.5-fold isoflavone content than Danbaek original variety
- Faba bean was showed increased tyrosinase activity than soybean, lentil seeds. On the other hand, antioxidant assay such as DPPH, ABTS free radical scavenging activity, that was recorded highest lentil bean
C. A study on processing properties and component characteristic of soybean bred mutant lines.
- Analysis of functional enzyme activity and changed component levels after differential processing method in faba bean and lentil
D. Exploring cold tolerance genes in faba bean transcriptome
- Analysis of gamma-irradiated Faba bean transcriptome by using PAC-bio sequencing
- Selected 6 cold tolerance candidate genes from EMBL-EBI database
- Faba bean transcriptome were 96 contig related to cold tolerance gene .

(출처 : SUMMARY 10p)