초록 ▼

Ca²⁺ 신호전달 네트워크 연구를 통해 골격근에서 운동에 의한 세포내 칼슘신호체계 연구를 통해 운동의 인슐린 저항성 개선 기전을 규명하고, 케라틴세포의 분화과정에서 칼슘 이차전령체인 NAADP의 역할 규명 및 패혈증에 의한 급성 간 손상에서 칼슘이차전령체인 NAADP에 의한 칼슘신호가 자가포식작용을 활성화하여 간 손상 보호기전 규명하고, 운동에 의한 근육 타입변화에 있어서 TRPM2 채널에 의한 칼슘증가 기전 규명 및 일산화탄소에 의한 FGF21의 발현 조절을 통한 대사조절 기전 규명하였다. 이에 대한 연구 결과

Ca²⁺ 신호전달 네트워크 연구를 통해 골격근에서 운동에 의한 세포내 칼슘신호체계 연구를 통해 운동의 인슐린 저항성 개선 기전을 규명하고, 케라틴세포의 분화과정에서 칼슘 이차전령체인 NAADP의 역할 규명 및 패혈증에 의한 급성 간 손상에서 칼슘이차전령체인 NAADP에 의한 칼슘신호가 자가포식작용을 활성화하여 간 손상 보호기전 규명하고, 운동에 의한 근육 타입변화에 있어서 TRPM2 채널에 의한 칼슘증가 기전 규명 및 일산화탄소에 의한 FGF21의 발현 조절을 통한 대사조절 기전 규명하였다. 이에 대한 연구 결과를 국제적인 학술지에 논문을 게재하고 특허도 국내는 물론 미국을 비롯한 해외에 출원하였다.

(출처 : 보고서 요약서 3p)

Abstract ▼

□ Purpose
The goal of this project is to construct a system that can select novel drug targets and search for cell/tissue-specific Ca²⁺ signal controllable drugs with fewer side effects by using Ca²⁺ signal road maps established in this laboratory. We ultimately want to deve

□ Purpose
The goal of this project is to construct a system that can select novel drug targets and search for cell/tissue-specific Ca²⁺ signal controllable drugs with fewer side effects by using Ca²⁺ signal road maps established in this laboratory. We ultimately want to develop therapeutic methods for human diseases through our understanding of integrated Ca²⁺ signaling systems.

□ contents
Since the abnormal action of Ca²⁺signals, which regulate many cellular processes, is the cause of various human diseases, the ability to accurately understand and control Ca²⁺ signals is of paramount importance towards the treatment of human diseases. In this study, we would like to establish the Ca²⁺ signaling network through our Ca²⁺ signal road maps, thereby developing Ca²⁺ signal control methods and a fundamental platform for the treatment of human diseases through the application of Ca²⁺ signal control methods to the disease-models. By elucidating the pathogenesis of human diseases based on the accurate understanding of Ca²⁺ signals, we would like to develop a new paradigm of Ca²⁺ signal control methods, with fewer side effects achieved through the search for cell/tissue-specific novel drug targets. Therefore, we propose the following goals regarding disease-related cells, the investigation of Ca²⁺ signaling, and the development of Ca²⁺ signal control methods.
(1) Study of Ca²⁺ signaling messenger generation
① Elucidation of the role of NOX in Ca²⁺ signaling messenger generation by CD38
② Elucidation of the role of NOS in Ca²⁺ signaling messenger generation by CD38
③ Elucidation of the structure and role of Non-CD38/non-BST-1 novel ADPRC
(2) Study of Ca²⁺ signaling in cells
① Elucidation of the role of ADPR by CD38 in the nucleus
② Study on Ca²⁺ signal control in cellular organelles (nucleus, mitochondria, lysosome)
③ Study on the role and control of Ca²⁺ signal in lysosome by NAADP in autophagy
④ Study on the role of TRPM2 in skeletal muscle
(3) Study of extracellular Ca²⁺ signaling network
① Study on immune-regulatory mechanism by sCD38
② Study on relation angiogenesis with pregnancy in PECAM-1-/- female mice
③ Study on Ca²⁺ signal control in keratinocytes by UVB
④ Study on Ca²⁺ signal control by carbon monoxide (CO)
⑤ Study on the regulation of cell function by exosomes in intercellular interaction

□ Developement results
As results of this research project, we have published several articles in various prestigious international journals and filed patents in our country as well as foreign countries, including the United States. As we described in the expected effects of this research, we will endeavor to enhance the effects of our research by publishing our papers in well-regarded journals and continuing to file patents.

□ Expected Contribution
Ca²⁺ signaling is the most fundamental signaling system in maintaining cellular processes. Hence, an accurate understanding of this signaling pathway is of immense scientific interest. We are striving towards becoming leaders in the international stage by providing the fundamental platform for the treatment of human diseases by elucidating the expression pattern in tissues and action mechanism of the Ca²⁺ signaling enzyme ADPRC through the Ca²⁺ signal roadmaps that we have developed from various cell types. The results of this research would contribute to the establishment of new treatment methods to ameliorate diabetes, liver cirrhosis, and male infertility due to the abnormal action of Ca²⁺ signalling. The successful fulfillment of this research will also lead to improvements in our socio-economic values, including the creation of new industries.

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 국 문 요 약 문 ... 4
• SUMMARY ... 5
• 목차 ... 6
• 1. 연구개발 목표 및 내용(연구계획서상에 작성된 목표 및 내용을 기술) ... 7
• 가. 최종목표 ... 7
• 나. 단계목표(당초목표 및 수정·보완 목표) ... 7
• 다. 당초 목표의 수정·보완(중요 연구변경) 사유 ... 7
• 라. 2단계 연차별 연구목표 및 내용 ... 9
• 2. 연구 추진전략 및 방법 ... 10
• 가. 연구 추진전략 ... 10
• 나. 단계별 연구내용 ... 11
• 다. 세부 연구방법 및 내용 ... 12
• 3. 주요 연구개발결과 ... 32
• 가. 계획대비 달성도 ... 32
• 나. 대표적 연구업적 ... 35
• 다. 현 단계 달성된 연구결과의 세계적 연구 위상 ... 36
• 라. 기타 계획하지 않은 연구성과 ... 38
• 마. 연구역량 향상 정도 ... 39
• 바. 세계적 연구리더로서 연구책임자의 성장 정도 ... 39
• 4. 연구수행에 따른 문제점 및 개선방향 ... 40
• 5. 연구개발성과 현황 ... 41
• 6. 국가과학기술종합정보시스템에 등록한 연구시설·장비 현황 ... 51
• 7. 기타사항 ... 51
• 끝페이지 ... 52