초록 ▼

바이오연료·소재 기술강국 실현을 위한 경쟁력 있는 원천기술 확보 및 통합생산시스템 구축
○ 1핵심: 생태친화형 고성능 미세조류 개발
- 고성능 미세조류 발굴, 개량 및 실증: 오일 생산성 >0.4 g/L/d
○ 2핵심: 바이오매스 대량 확보 및 바이오수송연료 생산 기술 개발
- 오일 및 고부가 물질의 생산 균주 배양, 수확, 추출 공정 기술 개발
- 항공유 및 기타 바이오연료 (그린디젤 등) 생산공정 개발 및 실증
○ 3핵심: 바이오소재 생산 기술 개발
- 3-HP, fucose, fucosyl

바이오연료·소재 기술강국 실현을 위한 경쟁력 있는 원천기술 확보 및 통합생산시스템 구축
○ 1핵심: 생태친화형 고성능 미세조류 개발
- 고성능 미세조류 발굴, 개량 및 실증: 오일 생산성 >0.4 g/L/d
○ 2핵심: 바이오매스 대량 확보 및 바이오수송연료 생산 기술 개발
- 오일 및 고부가 물질의 생산 균주 배양, 수확, 추출 공정 기술 개발
- 항공유 및 기타 바이오연료 (그린디젤 등) 생산공정 개발 및 실증
○ 3핵심: 바이오소재 생산 기술 개발
- 3-HP, fucose, fucosyllactose, 오메가 3 지방산, 2,3-BDO의 생산공정 개발

(출처 : 보고서 요약서 4p)

Abstract ▼

□ Purpose & Contents
○ Core 1: Developing eco-friendly and high-performing algal strains
● Optimizing the production of biomass (microalgae) and lipid via omics-based physiological and ecological studies on microalgae
● Analyzing microalgal genome and developing algal transformants and reco

□ Purpose & Contents
○ Core 1: Developing eco-friendly and high-performing algal strains
● Optimizing the production of biomass (microalgae) and lipid via omics-based physiological and ecological studies on microalgae
● Analyzing microalgal genome and developing algal transformants and recombinants exhibiting high growth rates and lipid contents
○ Core 2: Producing biomass at large-scale and developing technologies for converting biomass into bio-transportation fuels
● Developing cultivation technologies to achieve dense algal culture
● Developing algal harvesting process
● Developing microalgal oil (MAO) extraction process
● Developing the process of converting MAO into biofuels
● Designing and optimizing overall processes via system simulation
○ Core 3: Developing technologies for producing bio-materials
● Developing the process of producing microalgae-derived 3-hydroxypropionic (3-HP) acid
● Developing the microbial biotransformation process of producing fucosyllactose
● Developing the process of producing microalgae-derived omega-3 polyunsaturated fatty acids (DHA, EPA)
● Developing the process of producing microalgae-derived 2,3-Butanediol(2,3-BDO)

□ Results
○ Developing large-scale algal cultivation platform and the standard set of practices for its operation
○ Development of high density and low cost photo-bioreactors
○ Inventing low-energy and high-efficiency harvesting technology
○ Optimizing lipid extraction, purification, and conversion processes
○ Demonstration of algae-derived biofuel
○ Development of lipid-extracted biomass utilization technology
○ Optimization of expression of pathway enzymes by dynamic promoters:
○ Development of promoter library with varying dynamic range
○ Development of 3-HP tolerant strain
○ Development of efficient and stable enzymes for AldH using site-directed mutagenesis and directed evolution. Study of crystal structure
○ Optimization of coenzyme B12 synthetic pathway: Development of genome engineering tool box. Characterization and removal of riboswitches
○ Improvement of Titer, rate and yield

□ Expected Contribution
○ Securing indigenous algal strains that thrive in the unique environmentalconditions of Korean peninsular
○ Enabling further research on the cultivation and oil-extraction of the algal strains exhibiting high growth rates and lipid accumulation
○ Systematic integration of microalgal cultivation, harvesting, and oil extraction will play a key role in the commercialization of biofuels by significantly reducing overall costs
○ Developing microalgal oil conversion catalyst and process will enable Korea to attain competitive edge in the production of bio-based aviation fuels.
○ Constructing the integrative process system and database will provide important platforms for ABC to play a critical role in global research initiatives
○ Utilizing lipid-extracted microalgal biomass and glycerol will improve the economic outlook of biofuels
○ Process technologies for the production of biomaterials will enable the flourishing of microalgae-based biorefinery in Korea.
○ The development of novel technologies will provide chances to obtain relevant intellectual property rights and to transfer the technologies to industrial sector

(출처 : SUMMARY 10p)

목차 Contents

• 표지 ... 1
• 제 출 문 ... 2
• 보고서 요약서 ... 3
• 요약문 ... 5
• SUMMARY ... 10
• 목차 ... 11
• 1. 연구개발과제의 개요 ... 12
• 가. 연구개발 목적 ... 12
• 나. 연구개발의 필요성 ... 13
• 다. 연구개발 범위 ... 17
• 2. 연구수행내용 및 성과 ... 24
• 2.1 연구수행 내용 및 결과 ... 24
• 2.2 연구성과 ... 1680
• 3. 목표 달성도 및 관련 분야 기여도 ... 1681
• 3-1. 연구목표 ... 1681
• 3-2. 목표 달성여부 ... 1691
• 3-3. 목표 미달성 시 원인(사유) 및 차후대책(후속연구의 필요성 등) ... 1728
• 4. 연구개발성과의 활용 계획 등 ... 1729
• 5. 참고문헌 ... 1739
• 끝페이지 ... 1786