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Kafe 바로가기주관연구기관 | 한양대학교 HanYang University |
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연구책임자 | 계명찬 |
참여연구자 | 심성한 |
보고서유형 | 최종보고서 |
발행국가 | 대한민국 |
언어 | 한국어 |
발행년월 | 2019-07 |
과제시작연도 | 2019 |
주관부처 | 보건복지부 [Ministry of Health & Welfare(MW)(MW) |
등록번호 | TRKO202000003138 |
과제고유번호 | 1465028890 |
사업명 | 질환극복기술개발(R&D) |
DB 구축일자 | 2020-05-16 |
키워드 | 남성 난임.정자.유전체 분석.난임 진단.가임력 증진.Male infertility.Sperm.Genome analysis.Diagnostic marker.Fertility promotion. |
1. 정자의 미동정 신호전달 단백질의 기능 및 조절기 기전 해석
2. 나노입자를 이용한 정자의 수정능력 진단
3. 정자의 수정능력 증진
4. 불임환자의 유전제 분석을 통한 진단 마커 개발
5. 불임환자의 불임유발 원인 유전자 규명
(출처 : 요약서 3p)
Purpose&Contents
Purpose
⦁Understanding the function of unidentified signal proteins in sperms
⦁Sensing the marker proteins based on nanoparticles
⦁Genomic analysis of the patients with non-obstructive azoospermia
⦁Discovery of genes related to male infertility in the pedigree of pati
Purpose&Contents
Purpose
⦁Understanding the function of unidentified signal proteins in sperms
⦁Sensing the marker proteins based on nanoparticles
⦁Genomic analysis of the patients with non-obstructive azoospermia
⦁Discovery of genes related to male infertility in the pedigree of patients
Contents
· Expression of HER2 and GSK3 after capacitation and acrosome reaction and phosphorylation of GSK3 in human and mouse sperm
· Protease analysis in mouse sperm and agarose gel based fluorescence analysis for GSK3 activity
· Expression of cubilin in developing male germ cells in testis
· Sperm capacitation analysis with nanoprobe.
· Effect of HER2 and GSK3 inhibition on GSK3 kinase activity and phosphorylation of GSK3, phosphotyrosine proteome and acrosome reaction and motility
· GSK3 and acrosine activity analysis with gel shift assay during capacitation
· Expression of IL-1 system in mouse sperm, epididymis and testis and human sperm.
· Expression of GSK3, Wnt2b and Wnt3a in mouse epididymis tissue and epididymis fluid
· Effect of IGF-1, Okadaic acid, Akt inhibitor and Wnt3a on sperm GSK3 and phosphotyrosine proteome
· Analysis of GSK3α/β protein and mRNA in normal and SCO human testis
· Interaction of GSK3α and PKA in mouse sperm
· Effect of calyculin A on motility and inhibitory phosphorylation of GSK3 in low motility human sperm
· Correlation of phosphorylation of GSK3α/β and sperm motility
· Inhibitory phosphorylation of GSK3 and PDHα1 after treatment of A23187 in mouse sperm
· Mitochondrial membrane potential measurement with TMRE assay during mouse sperm maturation in epididymis
· Effects of DBP exposure on sperm motility, acrosome reaction, tail bending, and GSK3 phosphorylation
· Development of in vitro activity assay of sperm-secreted enzymes in a microplate
· Development of cell-penetrating imaging technology based on sperm fertility and its secreted enzyme
· Characterization of DNA integrity, Y chromosome microdeletions, genomic variations in Korean patients with male infertility
· Identification of novel gene defects using the next generation sequencing analyses
· Y chromosome microdeletions were identified in about 26% of patients with male patients
· Fragmentation of sperm DNA was evaluated by LM-RT-PCR method and this method could be useful for analyzing sperm DNA fragmentation.
· Novel sequence variations in STAG3, EGR4, CFP1, DMRT1 were identified in patients with idiopathic male infertility.
· Novel sequence variations associated with SRY-negative XX male were identified using whole exome sequencing analysis.
· Novel sequence variations associated with azoospermia were identified using whole exome sequencing analysis.
Results
· Patent 2, patent application 1
· Publication : 8 SCI(E) and 1 PubMed indexed Journal
· Training: 1 Doctor and 4 Masters (+1 in Sep. 2019)
Expected Contribution
· Diagnosis for male infertility using functional proteins and proteases involved in sperm fertilization
· Expansion of diagnostic technology and medical service via development of diagnostic kit and medium for the sperm fertility
· Development of early diagnosis and treatment via elucidation of genetic causes in male infertility
· This R&D will give hope to couples with infertility and also contribute to national decline in population.
(출처 : SUMMARY 5p)
과제명(ProjectTitle) : | - |
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연구책임자(Manager) : | - |
과제기간(DetailSeriesProject) : | - |
총연구비 (DetailSeriesProject) : | - |
키워드(keyword) : | - |
과제수행기간(LeadAgency) : | - |
연구목표(Goal) : | - |
연구내용(Abstract) : | - |
기대효과(Effect) : | - |
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