[보고서]ICT 융합 기반 아토피피부염의 한의학적 관리 기술 개발

장현철

보고서 정보

주관연구기관

한국한의학연구원
Korea Institute of Oriental Medicine

연구책임자

장현철

참여연구자

박민철 , 김호경 , 박히준

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2018-06

주관부처

과학기술정보통신부
Ministry of Science and ICT

등록번호

TRKO202000006897

DB 구축일자

2020-09-12

키워드

아토피피부염.한의학.질환관리.임상연구.소양증.Atopic Dermatitis.Korean Medicine.Disease Management.Clinical Trial.Itch.

표 세부 연계와 역할
표 세부 구성
표 Keywords of Atopic Dermatitis Related to Digestive Disorder
표 Keywords of Atopic Dermatitis Related to Respiratory Disorder
표 Symptoms of Food Allergy
표 음식 알레르기에 대한 진단 방법
표 아토피피부염 환자 연령별 분포
표 환자기록 점검 기능의 활용 요구
표 환자기록의 차트 전송기능 활용 요구
표 실내외 환경에 따른 아토피피부염 원인군 (삼성서울병원)
표 아토피피부염 지침 정보
표 아토피피부염 지침에 기록된 예방, 원인, 치료와 관리 항목 현황
표 일상에서의 공기 접촉 환경
표 콘텐츠의 기능 특성에 따른 분석
표 SO inhibits TNFα/IFNγ-induced ICAM-1 expression in HaCaT cells, which play a critical role in the adhesion of THP-1 cells to HaCaT cells. A. Cell viability was measured was using the MTT assay. HaCaT cells were incubated in medium in the absence (medium alone) or presence of SO at the indicated concentrations for 24 h. B. Phase-contrast photograph of changes in HaCaT cel1s cellular morpology. Magnications × 100. Cells were pretreated with SO for 30 min, and then stimulated TNFα/IFNγ for 24 h. C. HaCaT cells were pretreated with SO for 30 min and then stimulated TNFα/IFNγ for 24 h to assay ICAM-1 protein levels by western blot. D. HaCaT cells were incubated with SO for 30min, followed by treatment with TNFα/IFNγ for 24 h. Then, fluorescently labeled THP-1cells were added to the HaCaT cells and incubated for 30min at 37 °C. After incubation, the cocultured cells were washed twice with PBS to remove the nonadherent cells. The adherent cells were counted as described under Materials and methods, and the data are expressed as a percentage of the control. The results are the means ± SD from three independent determinations per formed in triplicate (** P<0.01, *P<0.05). SO, Soshiho-tang; T + I, TNFα + IFNγ
표 SO suppresses the TNFα/IFNγ-induced NF-κB activation in HaCaT cells. Cells were pretreated with the indicated concentrations of SO for 15min and then stimulated with TNFα/IFNγ for 30 min. The level of IκBα protein in cytosol and NF-κB (p65) protein present in the nucleus were determined by western blot analysis, as described in Section 2. B. Immunofluorescence fluorescence microscopy showing NF-κB (p65) in HaCaT cells. After 24 hours of serum starvation. Representative images from three independent determinations are shown. Scale bar, 10 μm. C. The nuclear extracts were assayed for NF-κB (p65) activation by NF-κB (p65) Transcription Factor Assay. The results are the means ± SD from three independent determinations performed in triplicate (** P<0.01, *P<0.05)
$SO inhibits TNFα/IFNγ-induced HO-1 expression and activates NRF-2 in HaCaT cells. A. Cells were treated with indicated concentrations of SO for 24 h, and HO-1 protein levels were detected by western blot analysis. B. Cells were treated with SO for various lengths of times, and HO-1 protein levels were detected by western blot analysis. C and D. Cells were treated with SO for indicated concentrations or times, and then the activations of NRF-2 in the cytosolic and nuclear fractions were determined by Western blot analysis. The results were confirmed by repeated experiments$ 표 SO inhibits TNFα/IFNγ-induced HO-1 expression and activates NRF-2 in HaCaT cells. A. Cells were treated with indicated concentrations of SO for 24 h, and HO-1 protein levels were detected by western blot analysis. B. Cells were treated with SO for various lengths of times, and HO-1 protein levels were detected by western blot analysis. C and D. Cells were treated with SO for indicated concentrations or times, and then the activations of NRF-2 in the cytosolic and nuclear fractions were determined by Western blot analysis. The results were confirmed by repeated experiments
표 HO-1 expression mediates inhibitory effect of SO on TNFα/IFNγ-induced ICAM-1 expression and monocyte adhesion HaCaT cells. A. Cells were transfected with control (CTL) and HO-1 siRNAs. After 48 h, Q-PCR and immunoblotting were performed to analyze HO-1 mRNA and protein levels. B. HaCaT cells were transfected with control and HO-1 siRNA, and then pretreated with SO for 30 min, followed by incubation with TNFα/IFNγ for 24 h. The protein levels of ICAM-1 and HO-1 were determined by Western blot analysis. C. CTL and HO-1 siRNA-transfected HaCaT cells were pretreated with SO for 30 min, followed by treatment with TNFα/IFNγ for 24 h. Then, fluorescently labeled THP-1 cells were added to the HaCaT cells and incubated for 30 min at 37 °C. After incubation, the cocultured cells were washed twice with PBS to remove the nonadherant cells. The adherent cells were counted as described under Materials and methods, and the data are expressed as a percentage of the control. The results are the means ± SD from three independent determinations performed in triplicate (** P<0.01, *P<0.05)
표 HO-1 expression mediates inhibitory effect of SO on TNFα/IFNγ-induced FIL, IVL and LOR expression in HaCaT cells. A. Cells were pretreated with SO for 30 min, and then stimulated TNFα/IFNγ for 24 h. mRNA levels of FIL, IVL and LOR were measured by Q-PCR. B. Cells were pretreated with SO for 30 min, and then stimulated TNFα/IFNγ for 48h h. Protein levels of FIL, IVL and LOR were measured by western blot analysis
표 Cell viability was analyzed using MTT assay. HaCaT cells were incubated in presence of indicated various concentration (10-500 μg/ml) or in the absence (only DMEM medium) for 24 h (A). Phase contrast images were taken to observe the morphology changes and proliferation of HaCaT cells by TNF-α/IFN-γ stimulation and SST pretreatment (original magnification: ×100) (B). HaCaT cells were pretreated with the various concentrations of SST for 30 min and stimulated with TNF-α/IFN-γ (20 ng/ml) for 24 h. The protein level of ICAM-1 was determined by western blot (C). Similar to the previous experiment, HaCaT cells were treated with SST and TNF-α/IFN-γ, and fluorescently labeled THP-1 cells were added to HaCaT cells and cultured at 37 °C for 30 min. Then, the co-cultured cells were washed with PBS to remove the non-adherent cells. The adherent cells were counted, and the graph is expressed as a percentage of the control (D,E). The data are shown as the mean ± S.E.Ms of the independent experiments per formed in triplicate. ###p<0.001 vs no-treatment condition, ***p <0.001 vs only TNF-α/IFN-γ treatment condition
$HaCaT cells were treated with indicated various concentrations of SST for 24 h (A), or treated with 100 μg/ml of SST for various lengths of time (B), then HO-1 protein levels were detected using western blot. HaCaT cells were treated with SST for indicated concentrations for 24 h (C), or treated with 100 μg/ml of SST for various lengths of times (D). And then the activations of Nrf-2 in the cytosolic and nuclear fractions were analyzed using Western blot. The results were confirmed by three times repeated experiments$ 표 HaCaT cells were treated with indicated various concentrations of SST for 24 h (A), or treated with 100 μg/ml of SST for various lengths of time (B), then HO-1 protein levels were detected using western blot. HaCaT cells were treated with SST for indicated concentrations for 24 h (C), or treated with 100 μg/ml of SST for various lengths of times (D). And then the activations of Nrf-2 in the cytosolic and nuclear fractions were analyzed using Western blot. The results were confirmed by three times repeated experiments
표 HaCaT cells were transfected with control siRNA and HO-1 siRNA for 48 h. Then, the levels of HO-1 mRNA and protein were confirmed using real-time PCR and western blot (A). HaCaT cells were transfected with control siRNA and HO-1 siRNA, and incubated for 48 h. Then pretreated with SST for 30 min, followed by stimulation with TNF-α/IFN-γ for 24 h. The protein levels of ICAM-1, HO-1 and β-actin were determined using western blot (B). After transfected with control siRNA and HO-1 siRNA in HaCaT cells, cells were pretreated with SST for 30 min, followed by treatment with TNF-α/IFN-γ for 24 h. Then, fluorescently labeled THP-1 cells were added to the HaCaT cells and incubated for 30 min at 37 °C. After incubation, the co-cultured cells were washed with PBS to remove the non-adherent cells. The adherent cells were counted, and the graph is expressed as a percentage of the control (C,D). The data are shown as the mean ± S.E.Ms of the independent experiments per formed in triplicate. #p<0.05 vs no-treatment condition,$p <0.05 vs only TNF-α/IFN-γ treatment condition., **p <0.01 vs Control siRNA transfected condition
표 HaCaT cells were pretreated with the indicated various concentrations of SST for 15 min, then stimulated with TNF-α/IFN-γ (20 ng/ml) for 30 min. The level of p-IκBα protein in cytosol, and NF-κB protein present in the nucleus were examined using western blot (A). After serum-starvation in HaCaT cells for 24 h, expression of NF-κB p65 was observed by immunofluorescence fluorescence microscopy. Representative images of three independent images were displayed (B). Nuclear extracts were analyzed for NF-κB activation by NF-κB transcription factor analysis (C). The data are shown as the mean ± S.E.Ms of the independent experiments per formed in triplicate. ###p<0.001 vs no-treatment condition, **p<0.01, or ***p <0.001 vs only TNF-α/IFN-γ treatment condition
표 HaCaT cells were pretreated with SST for 30 min, and then stimulated with TNF-α/IFN-γ for 24 h. mRNA levels of FIL (A), IVL (B) and LOR (C) were measured by real-time PCR. Cells were pretreated with SST for 30 min, and then stimulated with TNF-α/IFN-γ for 48 h. Protein levels of FIL (D), IVL (E) and LOR (F) were measured by western blot
표 To examine severity, photographs of mouse hairless dorsal skin were taken before mice were sacrificed on the 18th day of the start experiment (A). The clinical severity score of all mouse dorsal skin lesions was assessed according to the standard evaluation criteria (B). The mice were sacrificed and the skin was removed. Dorsal skin thickness of each mouse was measured three times in micrometer (C). The spleen weights of 6 mice per group were measured electronically (D) and pictures were taken to aid visual comparisons (E). The cut dorsal skin was fixed in 10% formalin for one day, then cut into 6 μm sections and stained with hematoxylin and eosin (H&E). Photographs of each group represent images of all mice (n = 6). Images were taken at ×100 original magnification (F). ###p<0.001 vs Normal group, *p<0.05, **p<0.01, or ***p <0.001 vs DNCB-induced group
표 Nrf2, HO-1, ICAM-1 and NF-κB expression levels in dorsal skin tissue were analyzed by IHC staining using anti-Nrf2, anti-HO-1, anti-ICAM-1 and anti-NF-κB antibody. Images were taken at ×100 original magnification
표 Prescription of mahaenggamseog-tang (per pack)
표 Case 1: Clinical Progress of SCORAD Index & VAS
표 Case 2: Clinical Progress of PASI & VAS
표 Prescription of Chijabyukpi-tang
표 Clinical Progress of SCORAD index
표 Clinical Progress of SCORAD index
표 Clinical Progress of VAS
표 Clinical Progress of VAS
표 2015.09.14
표 2015.09.30
표 2015.10.26
표 2015.08.31
표 2015.09.07
표 아토피 관리 침치료기술 개발 IRB 승인
표 침치료 효과에 사용된 경혈과 비교경혈의 위치
표 침치료와 플라세보 군에서의 가려움증 감소 효과 비교
표 The changes of time above scratch threshold and area under the rating curve above the scratch threshold
표 Skin blood perfusion images using laser Dopper perfusion imaging
표 The changes of HF norm (Heart rate vadiability)
표 The changes of LF norm (Heart rate vadiability)
표 Functional connectivity of putamen and ACC using fMRI
표 Comparison of acupuncture perception questionnaire scores between the acupuncture and the placebo groups
표 진짜 피내침, 김샴 피내침, 링샴 피내침의 비교
표 진짜 피내침, 김샴 피내침, 링샴 피내침의 비교 모식도
표 진짜 피내침, 김샴 피내침, 링샴 피내침의 눈가림 지수
표 진짜 피내침과 김샴, 진짜 피내침과 링샴의 눈가림 지수 관계로 살펴본 눈가림 효과 해석
표 진짜, 김샴, 링샴 부착력 비교
표 이상 반응 발생률 비교
표 IRB 심의결과서
표 아토피 환자 침구의학적 관리를 위한 예비 임상연구의 흐름도. AD, 아토피 피부염 (atopic dermatitis); VA, 침처치군(Verum Acupuncture group); SA, 가짜침군(sham acupuncture group); wks, weeks
표 경락경혈기반 복모혈과 아토피 침 임상 증상과의 상관성
표 5-HT로 유도된 소양증에 대한 곡지(LI 11) 침 자극의 효과. (A) 30분 간 총 scratching 횟수. (B) Scratching 횟수의 5분 단위 time course 변화. (C) 30분 간 총 wiping 횟수. (D) Wiping 횟수의 5분 단위 time course 변화. * p < 0.05, ** p < 0.01, *** p < 0.005 vs. 5-HT group
표 α-MS로 유도된 소양증에 대한 곡지(LI 11) 침 자극의 효과. (A) 30분 간 총 scratching 횟수. (B) Scratching 횟수의 5분 단위 time course 변화. (C) 30분 간 총 wiping 횟수. * p < 0.05, ** p < 0.01, *** p < 0.005 vs. α-MS group
표 LP44로 유도된 소양증에 대한 곡지(LI 11) 침 자극의 효과. (A) 30분 간 총 scratching 횟수. (B) Scratching 횟수의 5분 단위 time course 변화. (C) 30분 간 총 wiping 횟수. * p < 0.05, ** p < 0.01, *** p < 0.005 vs. LP44 group
표 아토피 피부염 환자에서 침치료 전후 발현 차이를 보이는 혈청 내 miRNA 목록
표 아토피 피부염 환자에서 침치료에 따른 혈청 내 IgE의 농도 변화
표 Bio-Plex 분석법에 의한 아토피 피부염 환자들에서 침치료에 따른 혈청 내 사이토카인의 정량적 평가
표 MC903로 유도된 아토피성 피부염 동물모델에서 피부병변에 대한 침치료의 효과
표 MC903로 유도된 아토피성 피부염 동물모델에서 혈중 5-HT의 농도에 대한 침치료의 효과
표 MC903로 유도된 아토피성 피부염 동물모델에서 소양증에 대한 침치료의 효과
표 MC903로 유도된 아토피성 피부염 동물모델에서 피부 내 조직학적 변화에 대한 침치료의 효과
표 MC903로 유도된 아토피성 피부염 동물모델에서 피부 내 5-HT2 및 HTR7의 발현에 대한 침치료의 효과 A, 5-HT2A; B, 5-HT2B; C, 5-HT2C; D, HTR7
표 MC903로 유도된 아토피성 피부염 동물모델에서 불안 및 우울증 유사 행동에 대한 침치료의 효과 A, EPM; B, TST; C, OFT
표 개인별 환경 요인 정보를 획득하고 활용하기 위한 아토피피부염 관리 체계
표 대시보드, 점검일지, 일일점검, 건강기록 화면 예
표 공유된 환자 정보의 예
표 환자의 건강/검사결과 대시보드
표 환자의 건강/검사결과 기록 및 상세화면
표 아토피피부염과 허약관리
표 아토피피부염 일상점검 화면
표 삶의 질 점검 화면
표 식단기록, 일상감정, 대변/소변/수면 기록 화면
표 SCORAD, 인체도 기록 화면
표 증상 사진 기록 화면
표 가족 관리와 다수 아동을 지원
표 생활습관 모니터링을 위한 대시보드
표 SCORAD의 누적과 양상 모니터링
표 생활습관 관리를 위한 대응절차 가이드
표 목욕 습관에 따른 점검과 정보 제공 콘텐츠 예
표 기상청에서 제공한 지역 온도(파랑), 어린이집(검정), 가정(주황)의 온도 변화
표 기상청에서 제공한 지역 습도(파랑), 어린이집(검정), 가정(주황)의 습도 변화
표 환경공단에서 제공한 지역 미세먼지(파랑), 어린이집(검정), 가정(주황)의 미세먼지 변화
표 아토피피부염 아동의 가정내 실내 공기질 측정 예
표 실외 공기질에 따른 교육과 정보 제공 콘텐츠 예
표 주거 환경에 따른 교육과 정보 제공 콘텐츠 예
표 실내외 공기질의 변화: 실내 공기질 측정부에서 측정된 미세먼지(검정)와 기상청의 대기 미세먼지(파랑)를 비교하면 외부공기 차단 상태에서 환기로 (단기적) 실내 공기질 측정을 기반으로 하는 경고는 의미가 없음 저점으로 측정된 미세먼지의 양과 실내 활동으로 유발되어 측정되는 고점을 줄이는 방향으로 실내 공기질 관리가 필요함
표 미세먼지 측정을 통한 건강 관리 방법 개요도
표 정밀한 삶의 질 지수 관찰을 위한 연령별 삶의 질 설문 적용 (영아 : 2세 이하, 어린이 : 3세-18세 이하, 성인 : 19세 이상)
표 Comparison between AD Group of Elementary School Visit Education and AD Group of Hospital Visit Education
표 Mean±SD of Objective SCORAD, Subjective SCORAD and EASI According to AD Severity
표 Comparison of Atopy Recognition between Parents of AD Group and Non-AD Group
표 Comparison of Parents of AD Groups in Elementary School Visit Education and Hospital Visit Education
표 Mean±SD of FDLQI and DFI According to Atopic Dermatitis
표 Analysis of KISE Results
표 Analysis of CDI & BAI Results
표 소아의 면역글로불린 생성 (The Immune System, 3rd, Garland Science 2009)
표 소아 아토피피부염과 폐계 허약
표 PHR앱 사용자그룹 아토피 환아(AD) 허약평가 상위증상
표 PHR앱 아토피 환아(AD) 사용자그룹 허약 평가 상위 10위 증상의 계통별 분포
표 치료점수가 낮은 그룹의 허약 계통 분포* * 장지혜 외. 소아 아토피 피부염에 대한 임상적 고찰(2015)
표 PHR 앱의 식단관리 기록
표 PHR 앱의 일상감정 기록
표 PHR 앱의 자각증상 기록

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총연구비 (DetailSeriesProject) :	-
키워드(keyword) :	-
과제수행기간(LeadAgency) :	-
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내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 제목(한글), 저자명(한글), 발행일자, 전자원문, 초록(한글), 초록(영문) 관리번호, 제목(한글), 제목(영문), 저자명(한글), 저자명(영문), 주관연구기관(한글), 주관연구기관(영문), 발행일자, 총페이지수, 주관부처명, 과제시작일, 보고서번호, 과제종료일, 주제분류, 키워드(한글), 전자원문, 키워드(영문), 입수제어번호, 초록(한글), 초록(영문), 목차
저장형식	Text(ASCII format) Excel format
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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