Abstract ▼

Mastitis is the most common and costly disease of dairy cattle and is also the single most common reason for antimicrobial use in dairy herds. To control and prevent the disease effectively, there is a need to better understand the current status of mastitis that has long been a problem in Korean da

Mastitis is the most common and costly disease of dairy cattle and is also the single most common reason for antimicrobial use in dairy herds. To control and prevent the disease effectively, there is a need to better understand the current status of mastitis that has long been a problem in Korean dairy herds. The purpose of this study was to examine the frequency and antimicrobial susceptibilities of causative bacteria isolated from dairy cattle milk with somatic cell counts (SCC) of ≥200,000 cells/ml from 2012 to 2016. The main results of the investigations are summarized as follows

Antimicrobial resistance in mastitits pathogens
From 2012 to 2016, a total of 4,965 nationwide milk samples were examined from diary cows； the collected samples were tested for somatic cells； isolation of mastitis pathogens was conducted in 1,970 (39,6%) samples with more than 200,000 somatic cells/ml； no pathogen was found in 288 (5.8%) samples, and a total of 2,833 strains were isolated from 1,970 samples. Primary culture of milk samples was performed as described by the National Mastitis Council (NMC, 1999), and isolates were identified by the VITEK MS system (BioMerieux). Antimicrobial susceptibility testing was performed according to Clinical Laboratory Standards Institute guidelines us a Sensititre plate (KRNVF, Trek, UK)： S. aureus, E. coli, and S. chromogenes were most frequently detected. Antibiotic resistance of the isolates was tested； various resistance patterns were found by species； gram positive bacteria such as S. aureus and CNS showed the highest resistance to penicillin, and gram negative bacteria such as E. coli showed the highest resistance to tetracycline. These results show prevalence and trend of resistance from bacteria isolates originated from bovine mastitis. This information can help select appropriate drug agents for empirical therapy of bovine mastitis infection and also may be helpful to set the guidelines on prevention and control of this pathogen in milk production system.

(출처 : 영문초록 17p)

목차 Contents

• 표지 ... 1
• I. 연구배경 및 목표 ... 2
• 1. 연구배경 ... 2
• 2. 연구최종목표 ... 3
• 3. 연차별 연구개발 목표 및 내용 ... 3
• II. 연구방법 및 수행전략 ... 3
• 1. 재 료 ... 3
• 2. 연구전략 및 방법 ... 3
• III. 연구결과 ... 4
• 1. 유방염 주요 원인균 분포 및 항생제감수성 검사(2012~2016) ... 4
• 2. 유방염 주요 원인균 특성 조사 ... 8
• IV. 연구결과요약 ... 13
• 1. 젖소 유방염 주요 원인균 분리 및 동정 ... 13
• 2. 유방염 원인체에 대한 항생제 내성 실태조사 ... 13
• 3. 주요 유방염 원인균(대장균, 황색포도상구균) 특성 조사 ... 13
• V. 금후 추진계획 ... 13
• VI. 연구결과 활용실적 및 계획 ... 14
• VII. 연구사업 추진상 문제점 및 건의사항 ... 16
• VIII. 주요연구 변경사항 ... 16
• IX. 참고자료 ... 16
• Ⅹ. 영문초록 ... 17
• 끝페이지 ... 17