[보고서]연안 습지식물 비쑥과 가는갯능쟁이로부터 항염증 및 지방세포분화 조절 소재 개발과 기전연구

서영완

보고서 정보

주관연구기관

한국해양대학교
Korea Maritime University

연구책임자

서영완

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2020-05

과제시작연도

2019

주관부처

해양수산부
Ministry of Oceans and Fisheries

등록번호

TRKO202000031221

과제고유번호

1525009126

사업명

해양바이오전략소재개발및상용화지원(R&D)

DB 구축일자

2020-12-12

키워드

비쑥.가는갯능쟁이.항염증.지방세포.염생식물.Artemisia scoparia.Atriplex gmelini.Antiinflammation.Adipocyte.Halophyte.

표 전세계 과체중, 비만인구(WHO예상) (출처 : WHO 2015)
표 한국인의 주요 질병 현황 (출처: 2009, 보건복지부, 국민건강영양조사)
표 65세 이상 남녀 비만도 분포 (출처 : 질병관리본부 2012)
표 주요 국가의 비만율 (출처 : 경향신문 2012)
표 비쑥을 활용한 특허동향
표 세계 보충제 시장매출액 및 성장률
표 건강기능식품 산업현황
표 체지방감소에 도움을 주는 기능성 원료
표 가는갯능쟁이의 용매조건에 따른 추출
표 가는갯능쟁이의 추출시간과 횟수에 따른 추출
표 추출조건에 따른 가는갯능쟁이의 주정에 의한 추출(용매 300 mL)
표 비쑥의 용매조건에 따른 추출
표 비쑥의 추출시간과 횟수에 따른 추출
표 추출조건에 따른 비쑥의 주정(300 mL)에 의한 추출
표 Chemical structures of compounds 1 and 2 from the halophyte Artemisia scoparis
표 1H and 13C NMR spectral data for compound 1 isolated from A. scoparia
표 1H and 13C NMR spectral data for compound 2 isolated from A. scoparia
표 1H and 13C NMR spectrum for compound 1 isolated from A. scoparia
표 1H and 13C NMR spectrum for compound 2 isolated from A. scoparia
$Scavenging effects of crude extract and its solvent fractions from Artemisia scoparia collected in Ganghwa on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λemission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water$ 표 Scavenging effects of crude extract and its solvent fractions from Artemisia scoparia collected in Ganghwa on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λemission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water
$Scavenging effects of crude extract and its solvent fractions from Artemisia scorparia collected in Jeju on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λemission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water$ 표 Scavenging effects of crude extract and its solvent fractions from Artemisia scorparia collected in Jeju on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λemission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water
$Scavenging effects of crude extract and its solvent fractions from Artemisia scorparia collected in Suncheon on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λ emission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water$ 표 Scavenging effects of crude extract and its solvent fractions from Artemisia scorparia collected in Suncheon on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λ emission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water
$Scavenging effects of crude extract and its solvent fractions from Atriplex gmelinii collected in Ganghwa on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λ emission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water$ 표 Scavenging effects of crude extract and its solvent fractions from Atriplex gmelinii collected in Ganghwa on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λ emission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water
$Scavenging effects of crude extract and its solvent fractions from Atriplex gmelinii collected in Suncheon on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λ emission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water$ 표 Scavenging effects of crude extract and its solvent fractions from Atriplex gmelinii collected in Suncheon on intracellular ROS induced by hydrogen peroxide. After preincubation of the HT 1080 cells in 20 μM DCFH-DA, cells were treated with sample for 2 h. DCF fluorescence was measured following addition of 500 μM H2O2 at λexcitation = 485 nm and λ emission = 528 nm: (a) Crude extract; (b) n-Hexane; (c) 85% aq.MeOH; (d) n-BuOH; (e) Water
$Scavenging effects of crude extract and its solvent fractions from Artemisia scoparis collected in the different habitats on authentic peroxynitrite: (a) Ganghwa; (b) Jeju; (c) Suncheon$ 표 Scavenging effects of crude extract and its solvent fractions from Artemisia scoparis collected in the different habitats on authentic peroxynitrite: (a) Ganghwa; (b) Jeju; (c) Suncheon
$Scavenging effects of crude extract and its solvent fractions from Artemisia scoparia collected in the different habitat on peroxynitrite induced from the decomposition of SIN-1. (a) Ganghwa; (b) Jeju; (c) Suncheon$ 표 Scavenging effects of crude extract and its solvent fractions from Artemisia scoparia collected in the different habitat on peroxynitrite induced from the decomposition of SIN-1. (a) Ganghwa; (b) Jeju; (c) Suncheon
$Scavenging effects of crude extract and its solvent fractions from Atriplex gmelinii collected in the different habitats on authentic peroxynitrite: (a) Ganghwa; (b) Suncheon$ 표 Scavenging effects of crude extract and its solvent fractions from Atriplex gmelinii collected in the different habitats on authentic peroxynitrite: (a) Ganghwa; (b) Suncheon
$Scavenging effects of crude extract and its solvent fractions from Atriplex gmelinii collected in the different habitat on peroxynitrite induced from the decomposition of SIN-1. (a) Ganghwa; (b) Suncheon$ 표 Scavenging effects of crude extract and its solvent fractions from Atriplex gmelinii collected in the different habitat on peroxynitrite induced from the decomposition of SIN-1. (a) Ganghwa; (b) Suncheon
$Inhibitory effect of crude extract and its solvent fractions from Artemisia scorparia collected in the different habitats on NO production in LPS induced Raw 264.7 cells. Raw 264.7 macrophages cells were pretreated with samples for 1 h before stimulation with LPS (1 μ g/mL) for another 48 h : (a) Ganghwa; (b) Jeju; (c) Suncheon$ 표 Inhibitory effect of crude extract and its solvent fractions from Artemisia scorparia collected in the different habitats on NO production in LPS induced Raw 264.7 cells. Raw 264.7 macrophages cells were pretreated with samples for 1 h before stimulation with LPS (1 μ g/mL) for another 48 h : (a) Ganghwa; (b) Jeju; (c) Suncheon
표 Cytotoxic effect of reynosin (1) and santamarine (2) from Artemisia scorparai on Raw 264.7 cells. Raw 264.7 macrophages cells were pretreated with samples for 24 h
표 Inhibitory effect of reynosin (1) and santamarine (2) from Artemisia scorparia on NO production in LPS induced Raw 264.7 cells. Raw 264.7 macrophages cells were pretreated with samples for 1 h before stimulation with LPS (1 μg/mL) for another 48 h
표 Inhibitory effect of reynosin (1) and santamarine (2) from Artemisia scorparia on the mRNA expression of iNOS and pre-inflammatory cytokine such as TNF-α, IL-1β, and IL-6 in LPS (1 μg/mL)-stimulated Raw264.7 cells for 24 h
$Inhibitory effect of crude extract and its solvent fractions from Atriplex gmelinii collected in the different habitats on NO production in LPS induced Raw 264.7 cells. Raw 264.7 macrophages cells were pretreated with samples for 1 h before stimulation with LPS (1 μ g/mL) for another 48 h : (a) Ganghwa; (b) Suncheon$ 표 Inhibitory effect of crude extract and its solvent fractions from Atriplex gmelinii collected in the different habitats on NO production in LPS induced Raw 264.7 cells. Raw 264.7 macrophages cells were pretreated with samples for 1 h before stimulation with LPS (1 μ g/mL) for another 48 h : (a) Ganghwa; (b) Suncheon
표 Sequences of primer used for RT-PCR
표 Cytotoxic effect of crude extract from A. scorparia on 3T3-L1 cells. The data represent the mean±SD of three separate experiments
표 Effect of crude extract from A. scorparia on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid droplets. The data represent the mean±SD of three separate experiments
표 Effect of crude extract from A. scorparia on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Effect of crude extract from A. scorparia on the protein levels of MAPK pathway in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
$Cytotoxic effect of solvent fractions (H2O, n-BuOH, 85% aq.MeOH and n-hexane) from A. scorparia on 3T3-L1 cells. The data represent the mean±SD of three separate experiments$ 표 Cytotoxic effect of solvent fractions (H2O, n-BuOH, 85% aq.MeOH and n-hexane) from A. scorparia on 3T3-L1 cells. The data represent the mean±SD of three separate experiments
$Effect of H2O and n-BuOH fractions from A. scorparia on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments$ 표 Effect of H2O and n-BuOH fractions from A. scorparia on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments
$Effect of 85%aq.MeOH and n-hexane fractions from A. scorparia on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments$ 표 Effect of 85%aq.MeOH and n-hexane fractions from A. scorparia on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments
$Effect of H2O fraction from A. scorparia on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments$ 표 Effect of H2O fraction from A. scorparia on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
$Effect of n-BuOH fraction from A. scorparia on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments$ 표 Effect of n-BuOH fraction from A. scorparia on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
$Effect of 85%aq.MeOH fraction from A. scorparia on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments$ 표 Effect of 85%aq.MeOH fraction from A. scorparia on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
$Effect of n-hexane fraction from A. scorparia on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments$ 표 Effect of n-hexane fraction from A. scorparia on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
$Effect of 85% aq.MeOH fraction from A. scorparia on the protein levels of MAPK and AMPK pathway in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments$ 표 Effect of 85% aq.MeOH fraction from A. scorparia on the protein levels of MAPK and AMPK pathway in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Cytotoxic effect of reynosin (1) and santamarine (2) from A. scorparia and genistein on 3T3-L1 cells. The data represent the mean±SD of three separate experiments
표 Effect of reynosin (1) and santamarin (2) from A. scorparia and genisteinon the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining
표 Effect of reynosin (1) and santamarin (2) from A. scorparia and genistein on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments
표 Effect of reynosin (1) from A. scorparia on the mRNA expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Effect of reynosin (1) from A. scorparia on the protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, SREBPlc, and leptin in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Effect of santamarine (2) from A. scorparia on the mRNA expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Effect of santamarine (2) from A. scorparia on the protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, SREBPlc, and leptin in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Effect of genistein from A. scorparia on the mRNA expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Effect of genistein from A. scorparia on the protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Effect of reynosin (1) from A. scorparia on the protein levels of MAPK and AMPK pathway in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Effect of santamarine (2) from A. scorparia on the protein levels of MAPK and AMPK pathway in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 Cytotoxic effect of crude extract from A. gmelinii on 3T3-L1 cells. The data represent the mean±SD of three separate experiments
표 Effect of crude extract from A. gmelinii on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments
$Cytotoxic effect of solvent fractions (H2O, n-BuOH, 85% aq.MeOH and n-hexane) from A. gmelinii on 3T3-L1 cells. The data represent the mean±SD of three separate experiments$ 표 Cytotoxic effect of solvent fractions (H2O, n-BuOH, 85% aq.MeOH and n-hexane) from A. gmelinii on 3T3-L1 cells. The data represent the mean±SD of three separate experiments
$Effect of H2O and n-BuOH fractions from A. gmelinii on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments$ 표 Effect of H2O and n-BuOH fractions from A. gmelinii on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments
$Effect of 85%aq.MeOH and n-hexane fractions from A. gmelinii on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments$ 표 Effect of 85%aq.MeOH and n-hexane fractions from A. gmelinii on the lipid accumulation of differentiated 3T3-L1 adipocytes depicted by Oil red O staining and the quantification of the stain bound to lipid dropletes. The data represent the mean±SD of three separate experiments
$Effect of H2O fraction from A. gmelinii on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments$ 표 Effect of H2O fraction from A. gmelinii on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
$Effect of n-BuOH fraction from A. gmelinii on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments$ 표 Effect of n-BuOH fraction from A. gmelinii on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
$Effect of 85%aq.MeOH fraction from A. gmelinii on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments$ 표 Effect of 85%aq.MeOH fraction from A. gmelinii on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
$Effect of n-hexane fraction from A. gmelinii on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments$ 표 Effect of n-hexane fraction from A. gmelinii on the mRNA and protein expression levels of key adipogenic differentiation marker, PPARγ, C/EBPα, and SREBPlc in 3T3-L1 adipocyte. The data represent the mean±SD of three separate experiments
표 RP-HPLC 분석 조건
표 비쑥 지표성분의 함량
표 비쑥 지표성분 함량 비교 peak
표 30일간 비쑥 지표성분의 함량 분석
표 비쑥 지표성분 안정성 분석. (A) 2020년 1월 비쑥. (B) 2020 2월 비쑥(UV 348 nm)
표 지표성분의 특이성 확인. 비쑥의 190∼400 nm에서 UV 흡수 peak들의 비교
표 비쑥 지표물질 santamarine의 검량선 작성
표 비쑥 지표물질 (santamarine) 분석의 정밀성
표 비쑥 지표물질 (santamarine) 분석의 정확성

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연안 습지식물 비쑥과 가는갯능쟁이로부터 항염증 및 지방세포분화 조절 소재 개발과 기전연구 원문보기

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연안 습지식물 비쑥과 가는갯능쟁이로부터 항염증 및 지방세포분화 조절 소재 개발과 기전연구 원문보기

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