초록 ▼

- 혼합영양배양시스템에서 15 g/L의 글루코스를 탄소원으로 공급하였을 때 파라밀론 생산성(350 mg/L/d)과 함량(81.5%)이 가장 높게 나타남.
- 파라밀론 분석을 위해 동결건조 시료에 에탄올을 첨가하여 세포를 파쇄하고, SDS와 urea 용액으로 단백질 제거 및 세척 후 정량하는 방법이 가장 효율적임.
- 유글레나 추출물은 면역 증진 및 과다면역 완화 기능을 가지며, 당뇨병 질환에 대한 치료제로서의 가능성이 있음.
• 혼합영양배양된 유글레나 추출물은 NO 생성 억제능의 증가, 사이토카인 IL-1β와 TNF

- 혼합영양배양시스템에서 15 g/L의 글루코스를 탄소원으로 공급하였을 때 파라밀론 생산성(350 mg/L/d)과 함량(81.5%)이 가장 높게 나타남.
- 파라밀론 분석을 위해 동결건조 시료에 에탄올을 첨가하여 세포를 파쇄하고, SDS와 urea 용액으로 단백질 제거 및 세척 후 정량하는 방법이 가장 효율적임.
- 유글레나 추출물은 면역 증진 및 과다면역 완화 기능을 가지며, 당뇨병 질환에 대한 치료제로서의 가능성이 있음.
• 혼합영양배양된 유글레나 추출물은 NO 생성 억제능의 증가, 사이토카인 IL-1β와 TNF-α를 증가시킨 반면, 종속영양배양된 유글레나 추출물은 IL-1β, TNF-α, IL-6 생성 억제능을 보임.
• 혼합영양배양된 유글레나의 열수추출물은 α-Glucosidase를 저해시켰으며, 영양조건에 관계 없이 유글레나의 메탄올추출물은 농도의존적으로 당뇨병 관련 효소인 PTP1B를 저해하였으며, 열수추출물은 미백치료와 관련있는 tyrosinase 효소를 저해하였음

(출처 : 보고서 요약서 3p)

Abstract ▼

□ Purpose & Contents
- Development of highly efficient production technology for marine biomaterials paramylon for commercialization as immuno-functional cosmetics
‧ Optimization of cultivation conditions for productivity improvement of paramylon
‧ Development of efficient detection method

□ Purpose & Contents
- Development of highly efficient production technology for marine biomaterials paramylon for commercialization as immuno-functional cosmetics
‧ Optimization of cultivation conditions for productivity improvement of paramylon
‧ Development of efficient detection method on paramylon
‧ Assessment of toxicity and functionality on paramylon
- The first step is to achieve paramylon productivity more than 125 mg/L/d (50% compared to 0.25 g/L/d of Euglena biomass productivity in batch system). To this end, paramylon productivity from different cultivation conditions (photoauto-, hetero-, mixotrophic) will be assessed, then the assessent of the productivity from different carbon sources will be performed with the selected optimal cultivation condition.
- The second step is the development of the paramylon analysis method. The standard material will be set as a control group, and the extraction efficiency will be compared between the control and the unknowns. The target efficiency of the paramylon produced is greater than 100% of the standard.
- The third is the evaluation step of toxicity and immune functionality of the paramylon produced in this study. The goal is to obtain the evaluation result proving that the paramylon has no toxicity and does have the immune functionality with related biomarker chosen from the study.

□ Results
- Optimal nutrition conditions and carbon source selection for high efficiency production of paramylon
‧ For optimal growth of cell growth, biomass and paramylon, mixotrophic condition were found to be favorable than heterotrophic conditions, and the cultivation system with 15 g/L glucose as a carbon source was the most optimal condition.
‧ We achieved 350 mg/L/d of paramylon productivity and 81.5% of paramylon content, which are much higher than the research goal(paramylon productivity of 125 mg/L/d, paramylon content 50%)
- Efficient paramylon analysis method development
‧ We modified quantification method type, sample condition, and use of solvent for protein removal purpose to get a better paramylon analysis efficiency..
‧ With lyophilized samples, treatments by ethanol, SDS, and urea and quantifying the weight by gravimetric method was selected as an optimized method for paramylon analysis.
- Presenting the functional evaluation data of the produced paramylon
‧ The methanol extract of Euglena from mixotrophic cultivation showed the ability to inhibit nitric oxide production, and hot water extract of the sample increased the cytokines IL-1β and TNF-α, which are immune-related mediators, while methanol extract of heterotrophic cultivated Euglena showed IL-1β, TNF-α, and IL-6 production inhibitory ability
‧ The hot water extract of mixotrophic cultivated Euglena has a function of inhibiting α-Glucosidase causing diabetes disease.
‧ Regardless of nutritional conditions, Euglena's methanol extract was found to be capable of inhibiting the diabetes-related enzyme PTP1B in a concentration-dependent manner, and the hot water extract was shown to inhibit the tyrosinase enzyme related to whitening treatment.

□ Expected Contribution
- Paramylon, as a value-added product with immune functionality, can be used in various fields not only as functional cosmetics, but also as pharmaceuticals, feed additives, and raw materials for functional foods.
- The low-carbon green technology can be exported by accomplishing commercialization of value-added products from microalgae, taking advantages of eco-friendly low-cost biological proces.
‧ Expecting long-term plan for entering global market with the value- added product from domestic microalgae species
- Reduction of production costs of functional foods and generation of high profit through substitution effect of importing raw materials from overeseas
‧ Securing key technologies for highly efficient production and expecting to enter global markets through intellectual property rights and valuable material registration
- Contribution to the creation of high quality jobs through revitalization of industries related to production of value-added products from microalgae

(출처 : SUMMARY 5p)

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 보고서 요약서 ... 3
• 요 약 문 ... 4
• SUMMARY ... 5
• 목차 ... 7
• 제1장. 연구개발과제의 개요 ... 8
• 1. 연구개발 목적 ... 8
• 2. 연구개발의 필요성 ... 12
• 3. 연구개발 범위 ... 13
• 제2장. 국내외 기술 개발 현황 ... 14
• 1. 국내외 기술동향 및 수준 ... 14
• 2. 국내외 시장현황 ... 15
• 3. 국내외 관련기관 현황 ... 17
• 제3장. 연구 수행 내용 및 성과 ... 18
• 1. 파라밀론 생산에 필요한 최적배양조건 선정 ... 18
• 2. 파라밀론 분석법 효율 제고 ... 38
• 3. 바이오매스의 면역기능성 평가 ... 45
• 제4장. 목표 달성도 및 관련 분야 기여도 ... 60
• 1. 목표 달성도 ... 60
• 2. 관련 분야 기여도 ... 61
• 제5장. 연구개발성과의 활용계획 ... 62
• 제6장. 연구개발성과의 보안등급 ... 63
• 제7장. 연구시설·장비종합정보시스템에 등록한 연구시설·장비 현황 ... 64
• 제8장. 연구개발과제 수행에 따른 연구실 등의 안전 조치 이행 실적 ... 65
• 제9장. 연구개발과제의 대표적 연구 실적 ... 66
• 제10장. 기타 사항 ... 67
• 제11장. 참고 문헌 ... 68
• [참고자료] 유글레나 성분분석결과서 ... 70
• 끝페이지 ... 72