[보고서]고기능 나노구조체와 고성능 바이오리셉터의 최적 조립 연구: 이상적인 바이오나노 융합플랫폼 구현

강태준

[국가R&D연구보고서] 고기능 나노구조체와 고성능 바이오리셉터의 최적 조립 연구: 이상적인 바이오나노 융합플랫폼 구현
Study on the Optimized Assembly of High Functional Nanostructure and High Performance Bioreceptor: Construction of Ideal NanoBio Convergence Platform 원문보기

보고서 정보
주관연구기관	한국생명공학연구원 Korea Research Institute of Bioscience and Biotechnology
연구책임자	강태준
참여연구자	김홍기 , 문정 , 강현주
보고서유형	1단계보고서
발행국가	대한민국
언어	한국어
발행년월	2020-11
과제시작연도	2020
주관부처	과학기술정보통신부 Ministry of Science and ICT
연구관리전문기관	한국생명공학연구원 Korea Research Institute of Bioscience and Biotechnology
등록번호	TRKO202100002185
과제고유번호	1711125181
사업명	한국생명공학연구원연구운영비지원(R&D)(주요사업비)
DB 구축일자	2021-06-12
키워드	나노구조체.다중결합.바이오리셉터.인터페이싱.초고감도.Nanostructure.Multivalent Interaction.Bioreceptor.Interfacing.Ultrasensitive.

초록 ▼

· 논문 26편(Nat. Commun., Biomaterials, Biosens. Bioelectron. 등), 특허 2건
· 저온에서 금 나노구조체 합성 성공
· 내성 바이러스 검출을 위한 화학적(OHT), 생물학적(A4) 리셉터 확보
· 저온에서 은 나노구조체 합성 성공 및 반응 시간에 따른 크기 및 모양 조절 최적화
· 금 나노판, 나노입자 조합을 이용한 신규 SERS 플랫폼 개발
· Zwitterionic 고분자와 protein G를 이용한 항체고정화 기술 개발
· 금 나노판, 나

Abstract ▼

Ⅳ. Results
☐ 26 Papers (Nat. Commun., Biomaterials, Biosens. Bioelectron., etc.), 2 patents
☐ Synthesis of Au nanostructures at low temperature
☐ Development of OHT and A4 for the detection of drug-resistant virus
☐ Synthesis of Ag nanostructures at low temperature
☐ Development of novel SERS-active platform using Au nanostructures
☐ Antibody immobilization technique using zwitterionic polymer and protein G
☐ Diagnosis of drug-resistnat virus through the convergence of nanostructures and bioreceptors

(출처 : SUMMARY 5p)

목차 Contents

표지 ... 1
제 출 문 ... 2
보고서 요약서 ... 3
요 약 문 ... 4
SUMMARY ... 5
CONTENTS ... 6
목차 ... 7
제 1 장 연구개발과제의 개요 ... 8
1. 연구개발의 필요성 ... 8
2. 연구개발의 목표 및 내용 ... 9
제 2 장 국내외 기술개발 현황 ... 10
제 3 장 연구개발수행 내용 및 결과 ... 12
1. 정량적 연구개발 결과 ... 12
2. 정성적 연구개발 결과 ... 13
제 4 장 목표달성도 및 관련분야에의 기여도 ... 25
제 5 장 연구개발결과의 활용계획 ... 26
끝페이지 ... 27

표/그림 (18)

표 Schematic illustration of the vapor-phase synthesis of Au nanostructures (NSs) at low temperature. When the substrate was placed 15–20 mm away from AuCl, Au NPs were grown on the substrate. When the substrate was placed 3–5 mm away from AuCl, Au nanoplates were obtained on the substrate
표 X-ray photoelectron spectroscopy (XPS) spectra obtained for two substrates placed in or out of the heating zone. Substrate A was positioned approximately 10 mm away from AuCl, and substrate B was positioned approximately 250 mm away from the precursor (inset). The binding energy peaks corresponding to Au0 were measured on substrate A (magenta). Binding energy peaks corresponding to Au3+ and Cl were measured on substrate B (blue)
표 (a) Cross-sectional high-resolution transmission electron microscopy (HR-TEM) image of Au NPs on an FTO substrate. The Au NPs directly interfaced with the FTO substrate without any space; (b) TEM image of Au NPs detached from the FTO substrate; (c,d) HR-TEM images of Au NPs obtained from the (c) orange and (d) blue boxes of (b). Insets are fast Fourier transformation (FFT) patterns of the Au NPs
표 XRD spectrum of the Au NPs synthesized on an FTO substrate. The Au peaks are marked by arrows and the other peaks are signals related to the intrinsic substrate
$(a) SEM image of free-standing Au nanoplates synthesized on an FTO substrate. The inset is a magnified SEM image of the Au nanoplates; (b) TEM image and selected-area electron diffraction (SAED) pattern (inset) of the Au nanoparticles; and (c) cross-sectional HR-TEM image of the Au nanoplate$ 표 (a) SEM image of free-standing Au nanoplates synthesized on an FTO substrate. The inset is a magnified SEM image of the Au nanoplates; (b) TEM image and selected-area electron diffraction (SAED) pattern (inset) of the Au nanoparticles; and (c) cross-sectional HR-TEM image of the Au nanoplate
표 Schematic illustration of the pH1N1/H275Y mutant virus detection procedure using OHT-functionalized glass slides and urchin Au NPs. OHT-functionalized glass slides were incubated with pH1N1/H275Y mutant virus samples. Next, OHT-functionalized urchin Au NPs were reacted with the mutant virus captured on the glass slides, and SERS signals were measured
표 (a) SEM images of OHT-functionalized glass slides after the detection of control, WT virus (102 PFU), and pH1N1/H275Y mutant virus (102 PFU) samples. The scale bars denote 3 μm. (b) Plot of SERS intensity for the 1616 cm−1 band versus the concentration of influenza viruses (0, 1, 10, 102 , and 103 PFU). The data represent the average plus standard deviation from five measurements. (c) SERS spectra of MGITC obtained from glass slides after the detection of mutant and WT virus mixture samples
표 (a) Schematic illustration of pH1N1/H275Y mutant virus detection in human nasal fluid and saliva collected from healthy persons. (b, c) SERS spectra of MGITC obtained from glass slides after the detection of the pH1N1/H275Y mutant virus (blue spectra), WT virus (magenta spectra), and no virus (black spectra) in (b) nasal fluid and (c) saliva. The concentrations of the viruses were 104 PFU/mL. (d) SEM images of mutant virus particles captured on OHT-functionalized glass slides. A magnified SEM image shows the captured pH1N1/H275Y mutant virus with a diameter of ∼95 nm (blue box)
표 Schematic illustration of SERS-based immunoassay for I223R/H275Y pH1N1 using A4 antibody
표 SERS-based detection of I223R/H275Y pH1N1 using A4 antibody. (A) SERS spectra of MGITC obtained from NPs-on-plate structures in the presence of I223R/H275Y pH1N1 (blue) and wt pH1N1 (black). The number of both viruses is 1,500 PFU. (B) Plot of 1,175 cm-1 band intensity versus the number of virus. Data represent the average plus/minus standard deviation from 10 measurements
표 Ag nanoplates grown vertically on an r-cut sapphire substrate. (a and b) 45° tilted-view and (c and d) top-view SEM images of the nanoplates at low and high magnifications, respectively. (e) TEM image and SAED pattern of the nanoplate. (f) HRTEM image of the red square in (e). (g) Crosssectional TEM image of the nanoplate, which was cut along the plane perpendicular to the side of the nanoplate. (h) HRTEM image and FFT patterns of the cyan square in (g). Regions A, B, and C are distinguished by two twin planes. All zone axes of the three FFT patterns are directions. Yellow and cyan circles indicate the {200} and {111} reflections, respectively
표 Growth process of Ag nanoplates and their volumetric size evolution with reaction time on r-cut sapphire substrates. (a) 45° tilted-view SEM images showing the growth process: twinned triangular seed → twinned pentagonal seed → pentagonal nanoplate. These nanostructures were obtained at 2 min of reaction. Scale bars are 200 nm. 45° tilted-view SEM images of the nanoplates synthesized at (b) 5, (c) 15, (d) 30, (e) 60, and (f) 90 min, respectively. (g) Nanoplate volume [(Average area of the main plane of nanoplates)3/2] as a function of reaction time
표 (A) Screening for the H275Y NA specificity of the monoclonal phages from three rounds of panning by ELISA with HRP-conjugated αM13. Error bars = standard deviation (n = 3). (B) Amino acid sequence of 6E3. (C) SDS-PAGE analysis of purified 6E3 under reducing (R) and non-reducing (NR) conditions. Lane M: protein marker; HC: heavy chain; LC: light chain
표 (A) Plot of absorbance at 450 nm as a function of WT (black) and H275Y (blue) NA concentration. Error bars = standard deviation (n = 3). (B) Plot of absorbance at 450 nm versus proteins. Absorbance at 450 nm increased only in the presence of H275Y NA. Error bars = standard deviation (n = 3). (C, D) Docking poses and calculated binding free energies of the model epitopes for (C) WT and (D) H275Y NAs in the CDR of 6E3 antibody
표 Schematic procedure of ZW-DOPA/protein G coating and antibody immobilization onto a bare substrate
표 (a) Schematic procedures for pH1N1 detection with the naked eye using the ZW-DOPA/protein G-coated substrate. (b) Grayscale images of ZW-DOPA/protein G-coated (upper) and PDA/protein G-coated (lower) substrates after pH1N1 detection with the naked eye; the corresponding plot of 8-bit grayscale values on ZWDOPA/protein G-coated and PDA/protein G-coated substrates after pH1N1 detection (right). (c) Grayscale images of ZW-DOPA/protein G-coated poly(ethylene terephthalate) (PET) (left), polystyrene (PS) (middle), and poly(tetrafluoroethylene) (PTFE) (right) substrates after pH1N1 detection with the naked eye
표 (A) Schematic illustration of 6E3 antibody-mediated SERS immunoassay for WT and H275Y pH1N1 viruses. (B) SERS spectra of MGITC as a function of H275Y pH1N1 concentration (0, 102, 103, 104, and 105 PFU/mL). (C) Plot of integrated 1,615 cm-1 band intensity versus WT pH1N1 and H275Y pH1N1 virus concentrations. Error bars = standard deviation (n = 10)
표 (A) Schematic illustration of drug-resistant virus detection in human nasopharyngeal aspirate sample. (B) SERS spectra of MGITC as a function of H275Y pH1N1-spiked human nasopharyngeal aspirate samples. (C) Plot of integrated 1,615 cm-1 band intensity versus WT pH1N1-spiked, H275Y pH1N1-spiked, and control human nasopharyngeal aspirate samples. Error bars = standard deviation (n = 10)

연구자의 다른 보고서 :

참고문헌 (25)

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 보고서

해당 보고서가 속한 카테고리에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

과제명(ProjectTitle) :	-
연구책임자(Manager) :	-
과제기간(DetailSeriesProject) :	-
총연구비 (DetailSeriesProject) :	-
키워드(keyword) :	-
과제수행기간(LeadAgency) :	-
연구목표(Goal) :	-
연구내용(Abstract) :	-
기대효과(Effect) :	-

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 제목(한글), 저자명(한글), 발행일자, 전자원문, 초록(한글), 초록(영문) 관리번호, 제목(한글), 제목(영문), 저자명(한글), 저자명(영문), 주관연구기관(한글), 주관연구기관(영문), 발행일자, 총페이지수, 주관부처명, 과제시작일, 보고서번호, 과제종료일, 주제분류, 키워드(한글), 전자원문, 키워드(영문), 입수제어번호, 초록(한글), 초록(영문), 목차
저장형식	Text(ASCII format) Excel format
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증